Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by cAMP | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | free form, strong, not in the presence of excess Mg2+ | Oryctolagus cuniculus | |
EGTA | strong | Oryctolagus cuniculus | |
Increasing ionic strength | up to 0.4 M NaCl, weak | Oryctolagus cuniculus | |
additional information | no inhibition by 3',5'-cAMP; no inhibition by AMP | Oryctolagus cuniculus | |
phosphate | up to 0.1 M, weak | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | 0.2 | [rabbit white skeletal muscle myosin P-light chain] | 27°C, pH 7.6 | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
sarcoplasm | - |
Oryctolagus cuniculus | 16528 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | effect depends on Mg2+ concentration | Oryctolagus cuniculus | |
Mg2+ | requirement, varies with ATP-concentration, MgATP2- is the active substrate | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin light chain | Oryctolagus cuniculus | - |
ADP + myosin light chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
new Zealand white | - |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin light chain | - |
Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | ITP, GTP, CTP or UTP cannot replace ATP | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | not specific for 22 kDa and 15 kDa light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is casein | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrates are 15 kDa and 22 kDa light-chain or heavy-chain fractions of myosin from white skeletal muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is troponin | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | highly specific for regulatory or P-light-chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | highly specific for 18 kDa light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is molluscan adductor myosin | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | specific for 20 kDa light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is phosphorylase b | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is histone III-S from thymus | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
ATP + [rabbit white skeletal muscle myosin P-light chain] | - |
Oryctolagus cuniculus | ADP + [rabbit white skeletal muscle myosin P-light chain] phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 77000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 8.2 | about half-maximal activity at pH 5.7 and 8.2, with a small shoulder of 77% of maximal activity at 7-7.5 | Oryctolagus cuniculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
rapid inactivation below | Oryctolagus cuniculus |
6.3 | 8 | stable in 10% sucrose | Oryctolagus cuniculus |