Cloned (Comment) | Organism |
---|---|
expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in Spodoptera frugiperda Sf9 cells via baculovirus infection system, transient expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in COS-7 cells, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of isozymes mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus, structure-activity relationships of the enzyme C-terminus, overview | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the variable C-terminal extension of GRK6 constitutes a domain with autoinhibitory function involving residues D560, S566, and L576 of 3 inhibitory elements and an intervening stimulatory element | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [G-protein-coupled receptor] | Mus musculus | - |
ADP + [G-protein-coupled receptor]phosphate | - |
? | |
additional information | Mus musculus | the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
4 isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | the C-terminus of GRK6 is palmitoylated, not required for phosphorylation activity | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant isozymes mGRK6-A, mGRK6-B, and mGRK-C from Sf9 insect cells by sequential gel filtration and heparin affinity chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | light-activated rhodopsin, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C, the latter showing highest activity | Mus musculus | ADP + phosphorylated rhodopsin | - |
? | |
ATP + [G-protein-coupled receptor] | - |
Mus musculus | ADP + [G-protein-coupled receptor]phosphate | - |
? | |
additional information | the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain | Mus musculus | ? | - |
? | |
additional information | structure-activity relationships of the enzyme C-terminus, overview | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain consisting of 3 autoregulatory elements involving residues D560, S566, and L576 and an intervening stimulatory element | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
G-protein-coupled receptor kinase 6 | - |
Mus musculus |
GRK6 | - |
Mus musculus |
More | GRK6 belongs to the GRK4 subfamily of the GRK family | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |