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Literature summary for 2.7.11.14 extracted from
- Molecular recognition of rhodopsin kinase GRK1 and recoverin is tuned by switching intra- and intermolecular electrostatic interactions (2019), Biochemistry, 58, 4374-4385 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a peptide containing the first 25 amino acids of GRK1 (MDFGSLETVVANSAFIAARGSFDAS) plus GST-tag is expressed in Escherichia coli BL21 cells | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | Ca2+ controls the binding of recoverin to the enzyme | Bos taurus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P28327 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-Sepharose column chromatography | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| additional information | the enzyme is under specific control of the neuronal Ca2+-sensor protein recoverin. In the dark state of the photoreceptor cell, the Ca2+-saturated form of recoverin forms a complex with the enzyme, which prevents binding of the enzyme to rhodopsin | Bos taurus | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G protein-coupled receptor kinase 1 | - |
Bos taurus |
| GRK1 | - |
Bos taurus |
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