Literature summary for 2.7.11.14 extracted from

Grigoriev, I.; Senin, I.; Tikhomirova, N.; Komolov, K.; Permyakov, S.; Zerni, E.; Koch, K.; Philippov, P.
Synergetic effect of recoverin and calmodulin on regulation of rhodopsin kinase (2012), Front. Mol. Neurosci., 5, 28.

Search for more literature for 2.7.11.14

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a GST-fusion protein	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
DELTA1-102	deletion fragment containing the first 102 amino acids interacts with calmodulin	Bos taurus
DELTA1-25	recoverin interacts with the rhodopsin fragment containing the first 25 amino acids of the enzyme. Calmodulin does not interact with this fragment	Bos taurus
DELTA102-183	deletion fragment containing only amino acids 102-183 shows a very high interaction with calmodulin	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
Calmodulin	the high affinity calmodulin binding site is localized within a stretch of amino acid residues V150-K175 in the N-terminal regulatory region of rhodopsin kinase. The inhibitory effect of calmodulin and recoverin on rhodopsin kinase activity is synergetic	Bos taurus
recoverin	the inhibitory effect of calmodulin and recoverin on rhodopsin kinase activity is synergetic	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	regulation of the rhodopsin activity by recoverin and calmodulin is Ca2+-dependent	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + rhodopsin	-	Bos taurus	ADP + phosphorylated rhodopsin	-	?

Synonyms

Synonyms	Comment	Organism
GRK1	-	Bos taurus
rhodopsin kinase	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Bos taurus

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Release 2023.1 (January 2023)