Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA20-535 | deletion of 19 N-terminal amino acids results in increased flexibility in the active site and interdomain contacts of this enzyme: peptides that directly interact with ATP are not as highly stabilized by adding Mg2+-ATP, and dynamics are greater in the interface between the large lobe of the kinase domain and the regulator of the G protein signaling homology domain compared to wild-type | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | Bos taurus | conformational flexibility of GRK1 is reduced in the presence of either Mg2+-ATP or rhodopsin, with Mg2+ATP having the greatest effect | ADP + phosphorhodopsin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | conformational flexibility of GRK1 is reduced in the presence of either Mg2+-ATP or rhodopsin, with Mg2+ATP having the greatest effect | Bos taurus | ADP + phosphorhodopsin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GRK1 | - |
Bos taurus |