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Literature summary for 2.7.11.13 extracted from

  • Meng, Z.; Böhm, T.; Xu, P.; Henne-Bruns, D.; Peifer, C.; Witt, L.; Knippschild, U.; Bischof, J.
    Kinase activity of casein kinase 1 delta (CK1delta) is modulated by protein kinase Calpha (PKCalpha) by site-specific phosphorylation within the kinase domain of CK1delta (2019), Biochim. Biophys. Acta Proteins Proteom., 1867, 710-721 .
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters of wild type and mutant full-length and truncated GST-CK1delta fusion proteins Homo sapiens
0.00009141
-
 ATP pH 7.5, 30°C, T176A mutant GST-CK1delta fusion protein Homo sapiens
0.0000963
-
 ATP pH 7.5, 30°C, full-length GST-CK1delta fusion protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [casein kinase 1 delta] Homo sapiens casein kinase 1 delta is modulated by protein kinase C alpha by site-specific phosphorylation within the kinase domain of CK1delta ADP + phosphorylated casein kinase 1 delta
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P17252
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein casein kinase 1 delta is modulated by protein kinase C alpha (PKCalpha) by site-specific phosphorylation within the kinase domain of CK1delta Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein kinase 1delta mutations T161A, T174A, and T176A in casein kinase 1delta (CK1delta) all result in reduced phosphorylation by PKCalpha. Most significant effects are visible for GST-CK1delta98-178,T161A and GST-CK1delta98-178,T176A with about 55% and 46% reduction compared to wild type, whereas phosphorylation of GST-CK1delta98-178,T174A is only slightly reduced. Mutation S181A in GST-CK1delta178-227,S181A reduces phosphorylation by PKCalpha by 74%, while for S191A in GST-CK1delta178-227,S191A (FP1552) only a minor reduction can be observed. GST-CK1delta227-304,S298A show no clear reduction of PKCalpha-mediated phosphate incorporation Homo sapiens ADP + phosphorylated casein kinase 1delta
-
 ?
ATP + [casein kinase 1 delta] casein kinase 1 delta is modulated by protein kinase C alpha by site-specific phosphorylation within the kinase domain of CK1delta Homo sapiens ADP + phosphorylated casein kinase 1 delta
-
 ?

Synonyms

Synonyms Comment Organism
PKCalpha
-
 Homo sapiens
protein kinase C alpha
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters of wild type and mutant full-length and truncated GST-CK1delta fusion proteins Homo sapiens