BRENDA - Enzyme Database show
show all sequences of 2.7.1.77

Nucleoside phosphotransferase from barley. Characterization and evidence for ping pong kinetics involving phosphoryl enzyme

Prasher, D.C.; Carr, M.C.; Ives, D.H.; Tsai, T.C.; Frey, P.A.; J. Biol. Chem. 257, 4931-4939 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
Hordeum vulgare
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cd2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cu2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
EDTA
2 mM, 85% inhibition. 95% inhibition at 10 mM
Hordeum vulgare
Hg2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Zn2+
2 mM, 20% inhibition of transphosphorylation
Hordeum vulgare
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.087
-
deoxyadenosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.412
-
deoxyguanosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.89
-
deoxythymidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.13
-
guanosine
pH 5.0, reaction with AMP
Hordeum vulgare
3.93
-
uridine
pH 5.0, reaction with AMP
Hordeum vulgare
4.15
-
deoxycytidine
pH 5.0, reaction with AMP
Hordeum vulgare
6.39
-
cytidine
pH 5.0, reaction with AMP
Hordeum vulgare
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Co2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Mg2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation
Hordeum vulgare
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
2 * 50000, SDS-PAGE
Hordeum vulgare
53400
-
non-denaturing PAGE
Hordeum vulgare
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hordeum vulgare
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
carrying a variable amount of polysaccharide
Hordeum vulgare
Purification (Commentary)
Commentary
Organism
-
Hordeum vulgare
Reaction
Reaction
Commentary
Organism
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate
ping pong kinetics
Hordeum vulgare
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Hordeum vulgare
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
180
-
-
Hordeum vulgare
Storage Stability
Storage Stability
Organism
-20°C, stable for at least 6 months
Hordeum vulgare
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively
Hordeum vulgare
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641994
Hordeum vulgare
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 50000, SDS-PAGE
Hordeum vulgare
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Hordeum vulgare
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
phosphatase activity
Hordeum vulgare
6.5
-
transferase activity
Hordeum vulgare
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
8.5
pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
Hordeum vulgare
General Stability (protein specific)
General Stability
Organism
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
Hordeum vulgare
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cd2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cu2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
EDTA
2 mM, 85% inhibition. 95% inhibition at 10 mM
Hordeum vulgare
Hg2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Zn2+
2 mM, 20% inhibition of transphosphorylation
Hordeum vulgare
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.087
-
deoxyadenosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.412
-
deoxyguanosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.89
-
deoxythymidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.13
-
guanosine
pH 5.0, reaction with AMP
Hordeum vulgare
3.93
-
uridine
pH 5.0, reaction with AMP
Hordeum vulgare
4.15
-
deoxycytidine
pH 5.0, reaction with AMP
Hordeum vulgare
6.39
-
cytidine
pH 5.0, reaction with AMP
Hordeum vulgare
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Co2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Mg2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation
Hordeum vulgare
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
2 * 50000, SDS-PAGE
Hordeum vulgare
53400
-
non-denaturing PAGE
Hordeum vulgare
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
carrying a variable amount of polysaccharide
Hordeum vulgare
Purification (Commentary) (protein specific)
Commentary
Organism
-
Hordeum vulgare
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Hordeum vulgare
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
180
-
-
Hordeum vulgare
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for at least 6 months
Hordeum vulgare
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641994
Hordeum vulgare
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 50000, SDS-PAGE
Hordeum vulgare
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Hordeum vulgare
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
phosphatase activity
Hordeum vulgare
6.5
-
transferase activity
Hordeum vulgare
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
8.5
pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
Hordeum vulgare
Other publictions for EC 2.7.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672755
Birichevskaya
A comparison of enzymatic phos ...
Pantoea agglomerans
Biotechnol. Lett.
29
585-591
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
7
-
14
-
-
-
-
-
-
-
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1
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-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658328
Barai
An improved method for the enz ...
Pantoea agglomerans
Biotechnol. Lett.
26
1847-1850
2004
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
-
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-
-
1
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-
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
642002
Asano
A novel selective nucleoside p ...
Morganella morganii, Morganella morganii NCIMB10466
J. Biosci. Bioeng.
87
732-738
1999
-
-
-
1
-
-
7
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
7
-
2
-
-
2
-
-
-
-
1
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
642003
Shin
-
Characterization of nucleoside ...
Leishmania tropica
Nucleosides Nucleotides
14
13-22
1995
-
-
-
-
-
-
-
13
2
-
1
-
-
1
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
13
2
-
1
-
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641995
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
267-278
1986
-
-
-
-
-
-
-
8
-
-
2
-
-
3
-
1
1
-
-
2
1
-
28
1
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
2
-
-
-
1
1
-
2
1
-
28
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
641996
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
291-300
1986
6
-
-
-
-
1
3
-
-
6
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
1
-
3
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
642000
Tesoriere
The purification and propertie ...
Gallus gallus
Biochim. Biophys. Acta
786
231-244
1984
-
-
-
-
-
-
1
-
-
3
-
-
-
2
-
-
1
-
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
641994
Prasher
Nucleoside phosphotransferase ...
Hordeum vulgare
J. Biol. Chem.
257
4931-4939
1982
-
-
-
-
-
1
6
7
-
3
2
-
-
2
-
1
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
7
-
3
2
-
-
-
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
641999
Tesoriere
Kinetic properties of a nucleo ...
Gallus gallus
Biochim. Biophys. Acta
660
348-358
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
642001
Guranowski
Nucleoside phosphotransferase ...
Lupinus luteus
Biochim. Biophys. Acta
569
13-22
1979
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
641991
Brunngraber
Nucleoside phosphotransferase ...
Daucus carota
Methods Enzymol.
51
387-394
1978
1
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
1
1
1
10
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641997
Yuyama
Identification of true thymidi ...
Tetrahymena pyriformis, Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
-
-
-
-
-
-
-
-
1
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-
-
-
2
-
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2
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641993
Chao
Nucleoside phosphotransferase ...
Pantoea agglomerans
J. Biol. Chem.
251
2330-2333
1976
-
-
-
-
-
5
5
-
2
1
2
-
-
2
-
-
1
-
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
2
1
2
-
-
-
-
1
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
641138
Kit
Characterization of nucleoside ...
Gallus gallus
Arch. Biochem. Biophys.
169
66-76
1975
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641992
Rodgers
Nucleoside phosphotransferase ...
Daucus carota
J. Biol. Chem.
247
5448-5455
1972
-
-
-
-
-
-
3
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641990
Brunngraber
Purification and properties of ...
Daucus carota
J. Biol. Chem.
242
4834-4840
1967
-
-
-
-
-
2
8
-
-
3
1
-
-
2
-
-
1
-
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
3
1
-
-
-
-
1
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-