General Stability | Organism |
---|---|
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, at least 80% inhibition of transphosphorylation | Hordeum vulgare | |
Cd2+ | 2 mM, at least 80% inhibition of transphosphorylation | Hordeum vulgare | |
Cu2+ | 2 mM, at least 80% inhibition of transphosphorylation | Hordeum vulgare | |
EDTA | 2 mM, 85% inhibition. 95% inhibition at 10 mM | Hordeum vulgare | |
Hg2+ | 2 mM, at least 80% inhibition of transphosphorylation | Hordeum vulgare | |
Zn2+ | 2 mM, 20% inhibition of transphosphorylation | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
deoxyadenosine | pH 5.0, reaction with AMP | Hordeum vulgare | |
0.412 | - |
2'-deoxyguanosine | pH 5.0, reaction with AMP | Hordeum vulgare | |
0.89 | - |
2'-deoxythymidine | pH 5.0, reaction with AMP | Hordeum vulgare | |
2.13 | - |
guanosine | pH 5.0, reaction with AMP | Hordeum vulgare | |
3.93 | - |
uridine | pH 5.0, reaction with AMP | Hordeum vulgare | |
4.15 | - |
2'-deoxycytidine | pH 5.0, reaction with AMP | Hordeum vulgare | |
6.39 | - |
cytidine | pH 5.0, reaction with AMP | Hordeum vulgare |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement | Hordeum vulgare | |
Co2+ | 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement | Hordeum vulgare | |
Mg2+ | 2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement | Hordeum vulgare | |
Mg2+ | 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Hordeum vulgare |
53400 | - |
non-denaturing PAGE | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carrying a variable amount of polysaccharide | Hordeum vulgare |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate | ping pong kinetics | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180 | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 6 months | Hordeum vulgare |
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + 2'-deoxycytidine | - |
Hordeum vulgare | dCMP + adenosine | - |
? | |
3'-AMP + 2'-deoxyguanosine | - |
Hordeum vulgare | dGMP + adenosine | - |
? | |
3'-AMP + 2'-deoxythymidine | - |
Hordeum vulgare | dTMP + adenosine | - |
? | |
3'-AMP + cytidine | - |
Hordeum vulgare | CMP + adenosine | - |
? | |
3'-AMP + guanosine | - |
Hordeum vulgare | GMP + adenosine | - |
? | |
4-nitrophenyl phosphate + 2'-deoxyadenosine | - |
Hordeum vulgare | 4-nitrophenol + 2'-deoxyadenosine 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Hordeum vulgare |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
phosphatase activity | Hordeum vulgare |
6.5 | - |
transferase activity | Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity | Hordeum vulgare |