Data extracted from this reference:
General Stability
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
Hordeum vulgare
Inhibitors
Ca2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cd2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cu2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
EDTA
2 mM, 85% inhibition. 95% inhibition at 10 mM
Hordeum vulgare
Hg2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Zn2+
2 mM, 20% inhibition of transphosphorylation
Hordeum vulgare
KM Value [mM]
0.087
deoxyadenosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.412
deoxyguanosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.89
deoxythymidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.13
guanosine
pH 5.0, reaction with AMP
Hordeum vulgare
3.93
uridine
pH 5.0, reaction with AMP
Hordeum vulgare
4.15
deoxycytidine
pH 5.0, reaction with AMP
Hordeum vulgare
6.39
cytidine
pH 5.0, reaction with AMP
Hordeum vulgare
Metals/Ions
Ba2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Co2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Mg2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation
Hordeum vulgare
Molecular Weight [Da]
50000
2 * 50000, SDS-PAGE
Hordeum vulgare
53400
non-denaturing PAGE
Hordeum vulgare
Organism
Posttranslational Modification
glycoprotein
carrying a variable amount of polysaccharide
Hordeum vulgare
Purification (Commentary)
Reaction
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate
ping pong kinetics
Hordeum vulgare
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
-20°C, stable for at least 6 months
Hordeum vulgare
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively
Hordeum vulgare
Substrates and Products (Substrate)
4-nitrophenyl phosphate + 2'-deoxyadenosine
641994
Hordeum vulgare
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
Subunits
dimer
2 * 50000, SDS-PAGE
Hordeum vulgare
Turnover Number [1/s]
additional information
additional information
Hordeum vulgare
pH Optimum
5.5
phosphatase activity
Hordeum vulgare
6.5
transferase activity
Hordeum vulgare
pH Range
4.5
8.5
pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
Hordeum vulgare
General Stability (protein specific)
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
Hordeum vulgare
Inhibitors (protein specific)
Ca2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cd2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Cu2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
EDTA
2 mM, 85% inhibition. 95% inhibition at 10 mM
Hordeum vulgare
Hg2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
Zn2+
2 mM, 20% inhibition of transphosphorylation
Hordeum vulgare
KM Value [mM] (protein specific)
0.087
deoxyadenosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.412
deoxyguanosine
pH 5.0, reaction with AMP
Hordeum vulgare
0.89
deoxythymidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.13
guanosine
pH 5.0, reaction with AMP
Hordeum vulgare
3.93
uridine
pH 5.0, reaction with AMP
Hordeum vulgare
4.15
deoxycytidine
pH 5.0, reaction with AMP
Hordeum vulgare
6.39
cytidine
pH 5.0, reaction with AMP
Hordeum vulgare
Metals/Ions (protein specific)
Ba2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Co2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
Mg2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation
Hordeum vulgare
Molecular Weight [Da] (protein specific)
50000
2 * 50000, SDS-PAGE
Hordeum vulgare
53400
non-denaturing PAGE
Hordeum vulgare
Posttranslational Modification (protein specific)
glycoprotein
carrying a variable amount of polysaccharide
Hordeum vulgare
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
-20°C, stable for at least 6 months
Hordeum vulgare
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
4-nitrophenyl phosphate + 2'-deoxyadenosine
641994
Hordeum vulgare
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
Subunits (protein specific)
dimer
2 * 50000, SDS-PAGE
Hordeum vulgare
Turnover Number [1/s] (protein specific)
additional information
additional information
Hordeum vulgare
pH Optimum (protein specific)
5.5
phosphatase activity
Hordeum vulgare
6.5
transferase activity
Hordeum vulgare
pH Range (protein specific)
4.5
8.5
pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
Hordeum vulgare
Other publictions for EC 2.7.1.77
672755
Birichevskaya
A comparison of enzymatic phos ...
Pantoea agglomerans
Biotechnol. Lett.
29
585-591
2007
-
-
-
-
-
-
-
-
-
-
-
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-
2
-
-
1
-
-
-
7
-
14
-
1
-
-
-
-
-
-
-
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-
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-
-
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1
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7
-
14
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
658328
Barai
An improved method for the enz ...
Pantoea agglomerans
Biotechnol. Lett.
26
1847-1850
2004
-
1
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2
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1
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-
-
-
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-
-
-
-
-
-
-
-
642002
Asano
A novel selective nucleoside p ...
Morganella morganii, Morganella morganii NCIMB10466
J. Biosci. Bioeng.
87
732-738
1999
-
-
-
1
-
-
7
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
21
1
-
1
-
2
-
2
1
1
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-
-
-
-
-
-
1
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-
7
-
2
-
-
2
-
-
-
-
1
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
642003
Shin
-
Characterization of nucleoside ...
Leishmania tropica
Nucleosides Nucleotides
14
13-22
1995
-
-
-
-
-
-
-
13
2
-
1
-
-
1
-
-
-
-
-
-
1
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13
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13
2
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1
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1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641995
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
267-278
1986
-
-
-
-
-
-
-
8
-
-
2
-
-
3
-
1
1
-
-
2
1
-
28
1
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-
-
-
8
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
2
-
-
-
1
1
-
2
1
-
28
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
641996
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
291-300
1986
6
-
-
-
-
1
3
-
-
6
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
1
-
3
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
642000
Tesoriere
The purification and propertie ...
Gallus gallus
Biochim. Biophys. Acta
786
231-244
1984
-
-
-
-
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1
-
-
3
-
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2
-
-
1
-
-
3
1
-
1
1
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-
1
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
641994
Prasher
Nucleoside phosphotransferase ...
Hordeum vulgare
J. Biol. Chem.
257
4931-4939
1982
-
-
-
-
-
1
6
7
-
3
2
-
-
2
-
1
1
1
-
2
1
2
1
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
7
-
3
2
-
-
-
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
641999
Tesoriere
Kinetic properties of a nucleo ...
Gallus gallus
Biochim. Biophys. Acta
660
348-358
1981
-
-
-
-
-
-
-
-
-
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1
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2
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1
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2
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
642001
Guranowski
Nucleoside phosphotransferase ...
Lupinus luteus
Biochim. Biophys. Acta
569
13-22
1979
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
2
1
-
32
1
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-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
641991
Brunngraber
Nucleoside phosphotransferase ...
Daucus carota
Methods Enzymol.
51
387-394
1978
1
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
1
1
1
10
-
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641997
Yuyama
Identification of true thymidi ...
Tetrahymena pyriformis, Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
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1
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2
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2
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1
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-
2
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-
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-
-
-
-
-
-
-
-
641993
Chao
Nucleoside phosphotransferase ...
Pantoea agglomerans
J. Biol. Chem.
251
2330-2333
1976
-
-
-
-
-
5
5
-
2
1
2
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-
2
-
-
1
-
-
-
1
1
17
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
2
1
2
-
-
-
-
1
-
-
1
1
17
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
641138
Kit
Characterization of nucleoside ...
Gallus gallus
Arch. Biochem. Biophys.
169
66-76
1975
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3
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1
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2
1
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3
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2
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
641992
Rodgers
Nucleoside phosphotransferase ...
Daucus carota
J. Biol. Chem.
247
5448-5455
1972
-
-
-
-
-
-
3
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
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1
-
1
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-
3
-
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-
-
-
-
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-
3
-
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-
-
1
-
-
-
-
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-
1
-
-
1
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641990
Brunngraber
Purification and properties of ...
Daucus carota
J. Biol. Chem.
242
4834-4840
1967
-
-
-
-
-
2
8
-
-
3
1
-
-
2
-
-
1
-
-
1
1
2
16
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
3
1
-
-
-
-
1
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-