Nucleoside phosphotransferase from barley. Characterization and evidence for ping pong kinetics involving phosphoryl enzyme

Prasher, D.C.; Carr, M.C.; Ives, D.H.; Tsai, T.C.; Frey, P.A.; J. Biol. Chem. 257, 4931-4939 (1982)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Ca2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

Cd2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

Cu2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

EDTA

2 mM, 85% inhibition. 95% inhibition at 10 mM

Hordeum vulgare

Hg2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

Zn2+

2 mM, 20% inhibition of transphosphorylation

Hordeum vulgare

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.087

-

deoxyadenosine

pH 5.0, reaction with AMP

Hordeum vulgare

0.412

-

deoxyguanosine

pH 5.0, reaction with AMP

Hordeum vulgare

0.89

-

deoxythymidine

pH 5.0, reaction with AMP

Hordeum vulgare

2.13

-

guanosine

pH 5.0, reaction with AMP

Hordeum vulgare

3.93

-

uridine

pH 5.0, reaction with AMP

Hordeum vulgare

4.15

-

deoxycytidine

pH 5.0, reaction with AMP

Hordeum vulgare

6.39

-

cytidine

pH 5.0, reaction with AMP

Hordeum vulgare

Metals/Ions

Commentary

Organism

Structure

Ba2+

2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement

Hordeum vulgare

Co2+

2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement

Hordeum vulgare

Mg2+

2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation

Hordeum vulgare

Reaction

Commentary

Organism

Reaction ID

a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate

ping pong kinetics

Hordeum vulgare

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

4-nitrophenyl phosphate + 2'-deoxyadenosine

-

641994

Hordeum vulgare

4-nitrophenol + 2'-deoxyadenosine 5'-phosphate

-

-

-

-

Inhibitors

Commentary

Organism

Structure

Ca2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

Cd2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

Cu2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

EDTA

2 mM, 85% inhibition. 95% inhibition at 10 mM

Hordeum vulgare

Hg2+

2 mM, at least 80% inhibition of transphosphorylation

Hordeum vulgare

Zn2+

2 mM, 20% inhibition of transphosphorylation

Hordeum vulgare

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.087

-

deoxyadenosine

pH 5.0, reaction with AMP

Hordeum vulgare

0.412

-

deoxyguanosine

pH 5.0, reaction with AMP

Hordeum vulgare

0.89

-

deoxythymidine

pH 5.0, reaction with AMP

Hordeum vulgare

2.13

-

guanosine

pH 5.0, reaction with AMP

Hordeum vulgare

3.93

-

uridine

pH 5.0, reaction with AMP

Hordeum vulgare

4.15

-

deoxycytidine

pH 5.0, reaction with AMP

Hordeum vulgare

6.39

-

cytidine

pH 5.0, reaction with AMP

Hordeum vulgare

Metals/Ions

Commentary

Organism

Structure

Ba2+

2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement

Hordeum vulgare

Co2+

2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement

Hordeum vulgare

Mg2+

2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation

Hordeum vulgare

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

4-nitrophenyl phosphate + 2'-deoxyadenosine

-

641994

Hordeum vulgare

4-nitrophenol + 2'-deoxyadenosine 5'-phosphate

-

-

-

-

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

672755

Birichevskaya

A comparison of enzymatic phos ...

Pantoea agglomerans

Biotechnol. Lett.

29

585-591

2007

-

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2

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1

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7

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14

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1

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1

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7

-

14

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

658328

Barai

An improved method for the enz ...

Pantoea agglomerans

Biotechnol. Lett.

26

1847-1850

2004

-

1

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2

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-

642002

Asano

A novel selective nucleoside p ...

Morganella morganii, Morganella morganii NCIMB10466

J. Biosci. Bioeng.

87

732-738

1999

-

-

-

1

-

-

7

2

-

-

2

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-

2

-

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1

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1

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21

1

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1

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2

-

2

1

1

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1

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7

-

2

-

-

2

-

-

-

-

1

-

-

1

-

21

1

1

-

2

-

2

1

1

-

-

-

-

-

-

-

642003

Shin

-

Characterization of nucleoside ...

Leishmania tropica

Nucleosides Nucleotides

14

13-22

1995

-

-

-

-

-

-

-

13

2

-

1

-

-

1

-

-

-

-

-

-

1

-

13

-

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13

2

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1

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1

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13

-

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-

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-

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-

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-

-

-

-

641995

Billich

Nucleoside phosphotransferase ...

Hordeum vulgare

Biol. Chem. Hoppe-Seyler

367

267-278

1986

-

-

-

-

-

-

-

8

-

-

2

-

-

3

-

1

1

-

-

2

1

-

28

1

-

-

-

-

8

-

-

-

-

1

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-

-

-

-

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-

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1

8

-

-

2

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-

1

1

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2

1

-

28

1

-

-

-

8

-

-

-

-

-

-

-

-

-

-

641996

Billich

Nucleoside phosphotransferase ...

Hordeum vulgare

Biol. Chem. Hoppe-Seyler

367

291-300

1986

6

-

-

-

-

1

3

-

-

6

-

-

-

2

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

1

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-

-

-

-

6

-

-

-

-

-

1

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3

-

-

-

6

-

-

-

-

-

-

-

1

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-

-

-

1

-

1

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-

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-

-

-

642000

Tesoriere

The purification and propertie ...

Gallus gallus

Biochim. Biophys. Acta

786

231-244

1984

-

-

-

-

-

-

1

-

-

3

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2

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1

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3

1

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1

1

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1

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1

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-

-

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1

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3

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1

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3

1

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1

1

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1

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1

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-

641994

Prasher

Nucleoside phosphotransferase ...

Hordeum vulgare

J. Biol. Chem.

257

4931-4939

1982

-

-

-

-

-

1

6

7

-

3

2

-

-

2

-

1

1

1

-

2

1

2

1

1

-

-

-

-

1

2

1

-

-

-

-

-

-

-

-

-

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1

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6

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7

-

3

2

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-

1

1

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2

1

2

1

1

-

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-

1

2

1

-

-

-

-

-

-

-

-

641999

Tesoriere

Kinetic properties of a nucleo ...

Gallus gallus

Biochim. Biophys. Acta

660

348-358

1981

-

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1

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2

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1

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2

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1

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-

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-

-

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-

642001

Guranowski

Nucleoside phosphotransferase ...

Lupinus luteus

Biochim. Biophys. Acta

569

13-22

1979

-

-

-

-

-

1

-

2

-

-

1

-

-

1

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-

1

-

-

2

1

-

32

1

-

-

-

3

-

1

-

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-

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-

-

-

-

1

-

-

-

2

-

-

1

-

-

-

-

1

-

2

1

-

32

1

-

-

3

-

1

-

-

-

-

-

-

-

-

-

641991

Brunngraber

Nucleoside phosphotransferase ...

Daucus carota

Methods Enzymol.

51

387-394

1978

1

-

-

-

-

-

11

2

-

-

1

-

-

2

-

-

1

-

-

1

1

1

10

-

-

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-

-

1

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1

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3

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1

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-

-

-

-

11

-

2

-

-

1

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-

1

-

1

1

1

10

-

-

-

-

-

1

-

1

3

-

-

-

-

-

-

641997

Yuyama

Identification of true thymidi ...

Tetrahymena pyriformis, Tetrahymena pyriformis ST

Arch. Biochem. Biophys.

188

64-69

1978

-

-

-

-

-

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1

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2

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2

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1

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2

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641993

Chao

Nucleoside phosphotransferase ...

Pantoea agglomerans

J. Biol. Chem.

251

2330-2333

1976

-

-

-

-

-

5

5

-

2

1

2

-

-

2

-

-

1

-

-

-

1

1

17

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

5

-

-

2

1

2

-

-

-

-

1

-

-

1

1

17

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

641138

Kit

Characterization of nucleoside ...

Gallus gallus

Arch. Biochem. Biophys.

169

66-76

1975

-

-

-

-

-

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-

3

-

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1

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2

1

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3

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2

1

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-

641992

Rodgers

Nucleoside phosphotransferase ...

Daucus carota

J. Biol. Chem.

247

5448-5455

1972

-

-

-

-

-

-

3

-

-

-

1

-

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2

-

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1

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1

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-

-

1

-

1

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3

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3

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1

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1

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1

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1

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1

3

-

-

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641990

Brunngraber

Purification and properties of ...

Daucus carota

J. Biol. Chem.

242

4834-4840

1967

-

-

-

-

-

2

8

-

-

3

1

-

-

2

-

-

1

-

-

1

1

2

16

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

2

-

8

-

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3

1

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-

1

-

1

1

2

16

-

-

-

-

-

1

1

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-