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Information on EC 2.7.1.77 - nucleoside phosphotransferase
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2.7.1.77 nucleoside phosphotransferaseIUBMB Comments
Phenyl phosphate and nucleoside 3'-phosphates can act as donors, although not so well as nucleoside 5'-phosphates. Nucleosides as well as 2'-deoxyribonucleosides can act as acceptors.
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Word Map
- 2.7.1.77
-
nocturnal
-
penile
-
tumescence
-
nasal
-
provocation
-
ensemble
-
erectile
- rhinitis
- neomycin
- allergen
-
erect
-
normalisation
-
monte
- impotence
-
services
-
carlo
-
healthcare
- kanamycin
-
prick
- neopterin
-
thematic
-
professionals
-
semi-structured
-
psychogenic
-
practitioner
- dermatophagoides
-
neuropsychological
- pteronyssinus
-
rhinomanometry
-
nonpharmacological
- rem
-
allergen-specific
-
kanamycin-resistant
-
lennard-jones
-
laparoscopy
-
stakeholder
-
organisational
-
rhinoconjunctivitis
-
nonpalpable
-
rhinometry
-
nonallergic
-
sneezing
- synthesis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
npt, nucleoside phosphotransferase, nonspecific nucleoside phosphotransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
nonspecific nucleoside phosphotransferase
-
-
-
-
NPT
nucleotide:3'-deoxynucleoside 5'-phosphotransferase
-
-
-
-
phosphotransferase, nucleoside
-
-
-
-
NPT
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate
ping pong kinetics
-
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
nucleotide:nucleoside 5'-phosphotransferase
Phenyl phosphate and nucleoside 3'-phosphates can act as donors, although not so well as nucleoside 5'-phosphates. Nucleosides as well as 2'-deoxyribonucleosides can act as acceptors.
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CAS REGISTRY NUMBER
COMMENTARY
9055-37-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2',3')-uridylic acid + uridine
uridine + UMP
-
about 10% of the activity with phenyl phosphate
-
-
?
3'-CMP + thymidine
cytosine + TMP
-
20% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
3'-dAMP + thymidine
TMP + 3'-deoxyadenosine
-
56% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
3'-TMP + thymidine
thymidine + TMP
-
19% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
4-nitrophenyl phosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine
4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine 5-phosphate
-
80% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-2(1H)pyridone
4-nitrophenol + 1-(beta-D-ribofuranosyl)-2(1H)pyridone 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-4(1H)pyridone
4-nitrophenol + 1-(beta-D-ribofuranosyl)-4(1H)pyridone 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-bromo-2'-deoxyuridine
4-nitrophenol + 2'-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxy-2'-methyluridine
4-nitrophenol + 2'-deoxy-2'-methyluridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol
4-nitrophenyl phosphate + 2'-deoxyadenosine
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxycytidine
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyguanosine
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyinosine
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-O-methylcytidine
4-nitrophenol + 2'-O-methylcytidine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2-thiouridine
4-nitrophenol + 2-thiouridine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxy-3'-methyluridine
4-nitrophenol + 3'-deoxy-3'-methyluridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
4-nitrophenol + 3'-deoxyadenosine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyinosine
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-fluoroinosine
4-nitrophenol + 3'-fluoro-IMP
-
-
-
-
?
4-nitrophenyl phosphate + 3'-O-methylcytidine
4-nitrophenol + 3'-O-methylcytidine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3-(beta-D-ribofuranosyl)uric acid
4-nitrophenol + 3-(beta-D-ribofuranosyl)uric acid 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-bromouridine
4-nitrophenol + 5-bromouridine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-chlorouridine
4-nitrophenol + 5-chlorouridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-hydroxyuridine
4-nitrophenol + 5-hydroxyuridine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-iodouridine
4-nitrophenol + 5-iodouridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 6-chloro-9-(beta-D-ribofuranosyl)purine
4-nitrophenol + 6-chloro-9-(beta-D-ribofuranosyl)purine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 7-methylguanosine
4-nitrophenol + 7-methylguanosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 9-(beta-D-ribofuranosyl)purine
4-nitrophenol + 9-(beta-D-ribofuranosyl)purine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + beta-L-2',3'-dideoxycytidine
4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
-
38% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-L-3'-deoxy-2',3'-didehydrothymidine
4-nitrophenol + beta-L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
60% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-L-3'-deoxy-3'-chlorothymidine
4-nitrophenol + beta-L-3'-deoxy-3'-chlorothymidine 5'-phosphate
-
90% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-L-thymidine
4-nitrophenol + beta-L-thymidine 5'-phosphate
-
35% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-pseudouridine
4-nitrophenol + beta-pseudouridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + deazauridine
4-nitrophenol + deazauridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + O2,2'-anhydro-beta-L-5-methyluridine
4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
-
65% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + thymidine 3'-[(4-nitrophenyl)-phosphate]
4-nitrophenol + thymidine 3'-[(4-nitrophenyl)-phosphate] 5'-phosphate
-
-
-
-
?
5'-adenylic acid + uridine
adenosine + UMP
-
70% of the activity with phenyl phosphate
-
-
?
5'-AMP + thymidine
adenosine + TMP
-
72% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-CMP + thymidine
cytidine + TMP
-
92% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-dCMP + thymidine
2'-deoxycytidine + TMP
-
46% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-dGMP + thymidine
TMP + 2'-deoxyguanosine
-
65% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-GMP + thymidine
TMP + guanosine
-
61% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
acetylphosphate + inosine
acetate + IMP
-
233% of the activity with diphosphate
-
-
?
adenosine 3',5'-bisphosphate + thymidine
adenosine 3'-phosphate + TMP
-
30% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
ADP + thymidine
TMP + AMP
-
50% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
AMP + inosine
adenosine + IMP
-
62% of the activity with diphosphate
-
-
?
ATP + thymidine
ADP + TMP
-
18% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
beta-glycerophosphate + uridine
glycerol + UMP
-
about 15% of the activity with phenyl phosphate
-
-
?
carbamoyl phosphate + inosine
carbamate + IMP
-
276% of the activity with diphosphate
-
-
?
D-ribose 5-phosphate + thymidine
D-ribose + TMP
-
13% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
D-ribose 5-phosphate + uridine
D-ribose + UMP
-
about 30% of the activity with phenyl phosphate
-
-
?
diphosphate + adenosine
phosphate + 5'-AMP
-
35% of the activity with inosine
-
?
diphosphate + cytidine
phosphate + 5'-AMP
-
29% of the activity with inosine
-
?
diphosphate + guanosine
phosphate + 5'-GMP
-
35% of the activity with inosine
-
?
diphosphate + uridine
phosphate + 5'-AMP
-
47% of the activity with inosine
-
?
diphosphate + xanthosine
phosphate + 5'-XMP
-
54% of the activity with inosine
-
?
glucose 6-phosphate + inosine
D-glucose + IMP
-
95% of the activity with diphosphate
-
-
?
p-nitrophenylphosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine
4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + beta-L-2',3'-dideoxycytidine
4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + L-3'-deoxy-2',3'-didehydrothymidine
4-nitrophenol + L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + L-3'-deoxy-3'-chlorothymidine
4-nitrophenol + L-3'-deoxy-3'-chlorothymidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + L-thymidine
4-nitrophenol + L-thymidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + O2,2'-anhydro-beta-L-5-methyluridine
4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
-
-
-
-
?
3'-AMP + uridine
adenosine + UMP
-
70% of the activity with phenyl phosphate
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol
-
-
5'- and 3'-nucleotide products in the approximate ratio of 2:1
?
4-nitrophenyl phosphate + adenosine
5'-AMP + 4-nitrophenol
-
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
-
115% of the activity with diphosphate
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
-
115% of the activity with diphosphate
-
-
?
4-nitrophenyl phosphate + thymidine
4-nitrophenol + TMP
-
24% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
-
115% of the activity with diphosphate
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
-
115% of the activity with diphosphate
-
-
?
phenylphosphate + inosine
IMP + phenol
-
164% of the activity with diphosphate
-
-
?
phenylphosphate + inosine
IMP + phenol
-
164% of the activity with diphosphate
-
-
?
tripolyphosphate + inosine
? + IMP
-
13% of the activity with diphosphate
-
-
?
tripolyphosphate + inosine
? + IMP
-
13% of the activity with diphosphate
-
-
?
additional information
?
-
-
the enzyme also exhibits phosphatase activity
-
-
?
additional information
?
-
-
protein carries both transfer and hydrolytic functions
-
-
?
additional information
?
-
-
protein carries both transfer and hydrolytic functions
-
-
?
additional information
?
-
-
no activity with L-adenosine, L-cytidine, L-ribofuranosylthymine, and 1-(beta-L-arabinofuranosyl)thymine
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ba2+
-
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Co2+
Cu2+
Mg2+
Co2+
-
enhances phosphatase activity considerably, but transferase activity to a small extent
Co2+
-
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Cu2+
-
enhances phosphatase activity considerably, but transferase activity to a small extent
Mg2+
-
enhances phosphatase activity considerably, but transferase activity to a small extent
Mg2+
-
contains one Mg2+ per dimeric enzyme molecule, the metal-free apoenzyme is inactive
Mg2+
-
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Mg2+
-
10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cysteine
-
2.5 mM, depresses both transferase and phosphatase activity by about 25%
glutathione
-
2.5 mM, depresses both transferase and phosphatase activity by about 25%
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0059
2'-deoxyadenosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0057
2'-deoxyinosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0071
3'-deoxyadenosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0084
3'-deoxyinosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0066
3'-fluoroinosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate