pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
1 * 21500-25000 + 1 * 21500-25000, subunits differ substantially in amino acid composition, SDS-PAGE, sedimentation equilibrium ultracentrifugation or sucrose density gradient centrifugation in presence of 6 M guanidine HCl
probably the free apoenzyme is converted by means of cooperative interactions between regulatory sites into an enzyme-nucleotide complex which is particularly stable at 37°C; unstable to heat, protected from inactivation by a large number of nucleotides
addition of aliphatic alcohols, 0.2 M butanol, propanol or ethanol or urea induces a much more rapid inactivation at 37°C of associated forms; unstable to heat, protected from inactivation by a large number of nucleotides
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dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity