BRENDA - Enzyme Database
show all sequences of 2.7.1.7

Purification and properties of the mannokinase from Escherichia coli

Sebastian, J.; Asensio, C.; Arch. Biochem. Biophys. 151, 227-233 (1972)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-fructose
competitive to glucose
Escherichia coli
D-mannosamine
competitive
Escherichia coli
D-mannose
competitive to glucose
Escherichia coli
D-mannose 6-phosphate
up to 20 mM no effect
Escherichia coli
L-sorbose
competitive
Escherichia coli
lyxose
competitive
Escherichia coli
MgADP-
-
Escherichia coli
Mn2+
at a Mn2+/ATP ratio of more than 2
Escherichia coli
additional information
up to 20 mM fructose 6-phosphate shows no inhibitory effect
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
MgATP2-
reaction with D-mannose
Escherichia coli
0.3
-
D-fructose
pH 7.5, 25°C
Escherichia coli
0.3
-
ITP
pH 7.5, 25°C
Escherichia coli
0.5
-
GTP
pH 7.5, 25°C
Escherichia coli
0.6
-
dATP
pH 7.5, 25°C
Escherichia coli
2
-
CTP
pH 7.5, 25°C
Escherichia coli
2
-
UTP
pH 7.5, 25°C
Escherichia coli
4
-
D-glucose
pH 7.5, 25°C
Escherichia coli
4
-
TTP
pH 7.5, 25°C
Escherichia coli
5
-
D-glucosamine
pH 7.5, 25°C
Escherichia coli
10
-
2-deoxyglucose
pH 7.5, 25°C
Escherichia coli
10
-
D-glucose
pH 7.5, 25°C
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
can partially replace Mg2+
Escherichia coli
Mg2+
required
Escherichia coli
Mn2+
can partially replace Mg2+
Escherichia coli
additional information
no activation with Ca2+, Sn2+, Cd2+
Escherichia coli
Ni2+
40% of the activity with Mg2+
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-10°C, stable for several months
Escherichia coli
4°C, stable for several days
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-deoxy-D-glucose
20% of the activity with D-mannose
641894
Escherichia coli
ADP + 2-deoxy-D-glucose 6-phosphate
-
-
-
?
ATP + 2-deoxy-D-mannose
no activity
641894
Escherichia coli
ADP + 2-deoxy-D-mannose 6-phosphate
-
-
-
-
ATP + D-fructose
D-mannose and D-fructose phosphorylated equally well
641894
Escherichia coli
ADP + D-fructose 6-phosphate
-
641894
Escherichia coli
?
ATP + D-glucosamine
5% of the activity with D-mannose
641894
Escherichia coli
ADP + D-glucosamine 6-phosphate
-
-
-
-
ATP + D-glucose
50% of the activity with D-mannose
641894
Escherichia coli
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-mannose
-
641894
Escherichia coli
ADP + mannose 6-phosphate
-
641894
Escherichia coli
?
ATP + mannoheptulose
no activity
641894
Escherichia coli
ADP + mannoheptulose 6-phosphate
-
-
-
-
CTP + D-mannose
15% of the activity with ATP
641894
Escherichia coli
CDP + D-mannose 6-phosphate
-
-
-
?
dATP + D-mannose
-
641894
Escherichia coli
dADP + D-mannose 6-phosphate
-
-
-
?
GTP + D-mannose
60% of the activity with ATP
641894
Escherichia coli
GDP + D-mannose 6-phosphate
-
-
-
?
ITP + D-mannose
as active as ATP
641894
Escherichia coli
IDP + D-mannose 6-phosphate
-
-
-
-
TTP + D-mannose
30% of the activity with ATP
641894
Escherichia coli
TDP + D-mannose 6-phosphate
-
-
-
?
UTP + D-mannose
60% of the activity with ATP
641894
Escherichia coli
UDP + D-mannose 6-phosphate
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
57
-
5 min, crude extract stable
Escherichia coli
61
-
5 min, crude extract, 10% loss of activity
Escherichia coli
64
-
5 min, crude extract, about 80% loss of activity
Escherichia coli
66
-
5 min, crude extract, complete loss of activity
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
5 min, 50% loss of activity, 15 min, complete loss of activity
Escherichia coli
5
10
25°C, 60 min, stable
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
D-mannosamine
pH 7.5, 25°C
Escherichia coli
0.1
-
MgADP-
-
Escherichia coli
2
-
L-sorbose
pH 7.5, 25°C
Escherichia coli
5
-
lyxose
pH 7.5, 25°C
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-fructose
competitive to glucose
Escherichia coli
D-mannosamine
competitive
Escherichia coli
D-mannose
competitive to glucose
Escherichia coli
D-mannose 6-phosphate
up to 20 mM no effect
Escherichia coli
L-sorbose
competitive
Escherichia coli
lyxose
competitive
Escherichia coli
MgADP-
-
Escherichia coli
Mn2+
at a Mn2+/ATP ratio of more than 2
Escherichia coli
additional information
up to 20 mM fructose 6-phosphate shows no inhibitory effect
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
D-mannosamine
pH 7.5, 25°C
Escherichia coli
0.1
-
MgADP-
-
Escherichia coli
2
-
L-sorbose
pH 7.5, 25°C
Escherichia coli
5
-
lyxose
pH 7.5, 25°C
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
MgATP2-
reaction with D-mannose
Escherichia coli
0.3
-
D-fructose
pH 7.5, 25°C
Escherichia coli
0.3
-
ITP
pH 7.5, 25°C
Escherichia coli
0.5
-
GTP
pH 7.5, 25°C
Escherichia coli
0.6
-
dATP
pH 7.5, 25°C
Escherichia coli
2
-
CTP
pH 7.5, 25°C
Escherichia coli
2
-
UTP
pH 7.5, 25°C
Escherichia coli
4
-
D-glucose
pH 7.5, 25°C
Escherichia coli
4
-
TTP
pH 7.5, 25°C
Escherichia coli
5
-
D-glucosamine
pH 7.5, 25°C
Escherichia coli
10
-
2-deoxyglucose
pH 7.5, 25°C
Escherichia coli
10
-
D-glucose
pH 7.5, 25°C
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
can partially replace Mg2+
Escherichia coli
Mg2+
required
Escherichia coli
Mn2+
can partially replace Mg2+
Escherichia coli
additional information
no activation with Ca2+, Sn2+, Cd2+
Escherichia coli
Ni2+
40% of the activity with Mg2+
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-10°C, stable for several months
Escherichia coli
4°C, stable for several days
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-deoxy-D-glucose
20% of the activity with D-mannose
641894
Escherichia coli
ADP + 2-deoxy-D-glucose 6-phosphate
-
-
-
?
ATP + 2-deoxy-D-mannose
no activity
641894
Escherichia coli
ADP + 2-deoxy-D-mannose 6-phosphate
-
-
-
-
ATP + D-fructose
D-mannose and D-fructose phosphorylated equally well
641894
Escherichia coli
ADP + D-fructose 6-phosphate
-
641894
Escherichia coli
?
ATP + D-glucosamine
5% of the activity with D-mannose
641894
Escherichia coli
ADP + D-glucosamine 6-phosphate
-
-
-
-
ATP + D-glucose
50% of the activity with D-mannose
641894
Escherichia coli
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-mannose
-
641894
Escherichia coli
ADP + mannose 6-phosphate
-
641894
Escherichia coli
?
ATP + mannoheptulose
no activity
641894
Escherichia coli
ADP + mannoheptulose 6-phosphate
-
-
-
-
CTP + D-mannose
15% of the activity with ATP
641894
Escherichia coli
CDP + D-mannose 6-phosphate
-
-
-
?
dATP + D-mannose
-
641894
Escherichia coli
dADP + D-mannose 6-phosphate
-
-
-
?
GTP + D-mannose
60% of the activity with ATP
641894
Escherichia coli
GDP + D-mannose 6-phosphate
-
-
-
?
ITP + D-mannose
as active as ATP
641894
Escherichia coli
IDP + D-mannose 6-phosphate
-
-
-
-
TTP + D-mannose
30% of the activity with ATP
641894
Escherichia coli
TDP + D-mannose 6-phosphate
-
-
-
?
UTP + D-mannose
60% of the activity with ATP
641894
Escherichia coli
UDP + D-mannose 6-phosphate
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
57
-
5 min, crude extract stable
Escherichia coli
61
-
5 min, crude extract, 10% loss of activity
Escherichia coli
64
-
5 min, crude extract, about 80% loss of activity
Escherichia coli
66
-
5 min, crude extract, complete loss of activity
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
5 min, 50% loss of activity, 15 min, complete loss of activity
Escherichia coli
5
10
25°C, 60 min, stable
Escherichia coli
Other publictions for EC 2.7.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
641754
Mukai
Characterization and molecular ...
Arthrobacter sp., Arthrobacter sp. KM
Appl. Environ. Microbiol.
69
3849-3857
2003
1
-
1
-
-
-
-
4
-
7
1
-
-
12
-
-
1
1
-
-
4
-
8
1
1
-
1
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
4
-
7
1
-
-
-
-
1
-
-
4
-
8
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
641891
Gardiol
Transport and catabolism of D- ...
Sinorhizobium meliloti, Sinorhizobium meliloti L5-30
J. Bacteriol.
151
1069-1072
1982
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641893
Coulombel
Identification and kinetic stu ...
Streptomyces sp.
Biochim. Biophys. Acta
706
117-122
1982
-
-
-
-
-
-
2
2
-
1
1
1
-
2
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
4
2
-
1
1
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641481
Anderson
-
D-fructose (D-mannose) kinase ...
Leuconostoc mesenteroides
Methods Enzymol.
42C
39-43
1975
-
-
-
-
-
-
-
2
-
-
1
2
-
1
-
-
1
-
-
-
1
1
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
2
-
-
-
1
-
-
1
1
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
641892
Sabater
Identification and properties ...
Streptomyces violaceoruber
Biochim. Biophys. Acta
284
406-413
1972
-
-
-
-
-
-
9
4
-
3
-
-
-
2
-
-
1
-
-
1
1
2
22
-
-
-
1
-
1
1
-
-
8
-
-
-
-
-
-
-
-
-
-
9
8
4
-
3
-
-
-
-
-
1
-
1
1
2
22
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
641894
Sebastian
Purification and properties of ...
Escherichia coli
Arch. Biochem. Biophys.
151
227-233
1972
-
-
-
-
-
-
9
12
-
5
-
-
-
2
-
-
1
-
-
-
1
2
13
-
-
-
4
-
1
-
2
-
4
-
-
-
-
-
-
-
-
-
-
9
4
12
-
5
-
-
-
-
-
1
-
-
1
2
13
-
-
-
4
-
1
-
2
-
-
-
-
-
-
-