Literature summary for 2.7.1.6 extracted from

Inagaki, E.; Sakamoto, K.; Obayashi, N.; Terada, T.; Shirouzu, M.; Bessho, Y.; Kuroishi, C.; Kuramitsu, S.; Shinkai, A.; Yokoyama, S.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii (2006), Acta Crystallogr. Sect. F, 62, 169-171.

Search for more literature for 2.7.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21 codon plus (DE3)-RIL	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
seeding method in combination with the sitting-drop vapour-diffusion method using 30%(v/v) PEG 400, 1 M D-galactose, 5 mM AMP-PNP, 5% (w/v) PEG 4000, 50 mM MgCl2 and 0.1 M MES pH 6.3.	Pyrococcus horikoshii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.132	-	ATP	in 50 mM HEPES-NaOH pH 7.5, at 50°C	Pyrococcus horikoshii
0.403	-	D-galactose	in 50 mM HEPES-NaOH pH 7.5, at 50°C	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Q column chromatography, HiTrap Phenyl FF column chromatography, and HiLoad 16/60 Superdex 75 gel filtration	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-galactose	-	Pyrococcus horikoshii	ADP + alpha-D-galactose 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
GALK	-	Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	very little activity at 37°C	Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.91	-	ATP	in 50 mM HEPES-NaOH pH 7.5, at 50°C	Pyrococcus horikoshii
0.91	-	D-galactose	in 50 mM HEPES-NaOH pH 7.5, at 50°C	Pyrococcus horikoshii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)