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Literature summary for 2.7.1.6 extracted from

  • Timson, D.J.; Reece, R.J.
    Sugar recognition by human galactokinase (2003), BMC Biochem., 4, 16.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.6

Protein Variants

Protein Variants Comment Organism
D46A no detectable activity Homo sapiens
E43A minor changes in kinetic parameters Homo sapiens
E43G 10fold drop in turnover number Homo sapiens
E43G/H44I not soluble Homo sapiens
H44A not soluble Homo sapiens
H44I not soluble Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: N-acetyl-D-galactosamine, L-arabinose, D-fucose, D-glucose Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 ATP mutant E43G, pH 8.0, 37°C Homo sapiens
0.034
-
 ATP wild-type, pH 8.0, 37°C Homo sapiens
0.035
-
 ATP mutant E43A, pH 8.0, 37°C Homo sapiens
0.14
-
 D-galactose mutant E43G, pH 8.0, 37°C Homo sapiens
0.97
-
 D-galactose wild-type, pH 8.0, 37°C Homo sapiens
1.9
-
 D-galactose mutant E43A, pH 8.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 expression in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-galactose
-
 Homo sapiens ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
 ?
ATP + D-galactose
-
 Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
 ?
additional information no substrate: N-acetyl-D-galactosamine, L-arabinose, D-fucose, D-glucose Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 D-galactose mutant E43G, pH 8.0, 37°C Homo sapiens
6.7
-
 D-galactose mutant E43A, pH 8.0, 37°C Homo sapiens
8.7
-
 D-galactose wild-type, pH 8.0, 37°C Homo sapiens