Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.6 extracted from

  • Dey, P.M.
    Galactokinase of Vicia faba seeds (1983), Eur. J. Biochem., 136, 155-159.
    View publication on PubMed
Search for more literature for 2.7.1.6

Inhibitors

Inhibitors Comment Organism Structure
3-mercaptoethanol
-
 Vicia faba
ADP
-
 Vicia faba
alpha-D-galactose 1-phosphate
-
 Vicia faba
Cu2+ 1 mM, complete inhibition Vicia faba
D-galactose 1-phosphate
-
 Vicia faba
Hg2+ 1 mM, complete inhibition Vicia faba
iodoacetamide 0.05 mM, complete inactivation Vicia faba
NEM 0.05 mM, complete inactivation Vicia faba
PCMB 0.005 mM, complete inactivation Vicia faba
Zn2+ 1 mM, complete inhibition Vicia faba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 D-galactose pH 7.3, 25°C Vicia faba
1.2
-
 D-galactosamine pH 7.3, 25°C Vicia faba
1.5
-
 ATP pH 7.5, 25°C Vicia faba
2.2
-
 2-deoxy-D-galactose pH 7.5, 25°C Vicia faba

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, 7% of the activation with Mg2+ Vicia faba
Co2+ divalent cation required, 70% of the activation with Mg2+ Vicia faba
Mg2+ divalent cation required, Mg2+ is the best activator Vicia faba
Mn2+ divalent cation required, 40% of the activation with Mg2+ Vicia faba
Ni2+ divalent cation required, 15% of the activation with Mg2+ Vicia faba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30900
-
 2 * 30900, SDS-PAGE Vicia faba
60000
-
 gel filtration Vicia faba

Organism

Organism UniProt Comment Textmining
Vicia faba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vicia faba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.258
-
-
 Vicia faba

Storage Stability

Storage Stability Organism
-30°C, stable for 2 months with only 5-10% loss of activity Vicia faba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-galactose
-
 Vicia faba ADP + 2-deoxy-D-galactose 1-phosphate
-
 ?
ATP + D-galactosamine
-
 Vicia faba ADP + D-galactosamine 1-phosphate
-
 ?
ATP + D-galactose
-
 Vicia faba ADP + alpha-D-galactose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30900, SDS-PAGE Vicia faba

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 45 pH 7.3, 50 mM phosphate buffer, 10 mM 2-mercaptoethanol, 5% glycerol, 60 min, stable Vicia faba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Vicia faba

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 25°C, 30 min, stable Vicia faba

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5
-
 D-galactose 1-phosphate pH 7.3, 25°C, competitive inhibition with galactose as the variable substrate Vicia faba
4.8
-
 ADP pH 7.3, 25°C, non-competitive with respect to ATP Vicia faba
8.5
-
 ADP non-competitive with respect to galactose Vicia faba

pI Value

Organism Comment pI Value Maximum pI Value
Vicia faba isoelectric focusing
-
 5.3