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Literature summary for 2.7.1.6 extracted from
- Galactokinase of Vicia faba seeds (1983), Eur. J. Biochem., 136, 155-159.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-mercaptoethanol | - |
Vicia faba |  |
| ADP | - |
Vicia faba | |
| alpha-D-galactose 1-phosphate | - |
Vicia faba | |
| Cu2+ | 1 mM, complete inhibition | Vicia faba | |
| D-galactose 1-phosphate | - |
Vicia faba | |
| Hg2+ | 1 mM, complete inhibition | Vicia faba | |
| iodoacetamide | 0.05 mM, complete inactivation | Vicia faba | |
| NEM | 0.05 mM, complete inactivation | Vicia faba | |
| PCMB | 0.005 mM, complete inactivation | Vicia faba | |
| Zn2+ | 1 mM, complete inhibition | Vicia faba | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
D-galactose | pH 7.3, 25°C | Vicia faba | |
| 1.2 | - |
D-galactosamine | pH 7.3, 25°C | Vicia faba | |
| 1.5 | - |
ATP | pH 7.5, 25°C | Vicia faba | |
| 2.2 | - |
2-deoxy-D-galactose | pH 7.5, 25°C | Vicia faba | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | divalent cation required, 7% of the activation with Mg2+ | Vicia faba | |
| Co2+ | divalent cation required, 70% of the activation with Mg2+ | Vicia faba | |
| Mg2+ | divalent cation required, Mg2+ is the best activator | Vicia faba | |
| Mn2+ | divalent cation required, 40% of the activation with Mg2+ | Vicia faba | |
| Ni2+ | divalent cation required, 15% of the activation with Mg2+ | Vicia faba | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30900 | - |
2 * 30900, SDS-PAGE | Vicia faba |
| 60000 | - |
gel filtration | Vicia faba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vicia faba | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vicia faba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Vicia faba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.258 | - |
- |
Vicia faba |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, stable for 2 months with only 5-10% loss of activity | Vicia faba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-galactose | - |
Vicia faba | ADP + 2-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactosamine | - |
Vicia faba | ADP + D-galactosamine 1-phosphate | - |
? | |
| ATP + D-galactose | - |
Vicia faba | ADP + alpha-D-galactose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30900, SDS-PAGE | Vicia faba |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | pH 7.3, 50 mM phosphate buffer, 10 mM 2-mercaptoethanol, 5% glycerol, 60 min, stable | Vicia faba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Vicia faba |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | 25°C, 30 min, stable | Vicia faba |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
D-galactose 1-phosphate | pH 7.3, 25°C, competitive inhibition with galactose as the variable substrate | Vicia faba | |
| 4.8 | - |
ADP | pH 7.3, 25°C, non-competitive with respect to ATP | Vicia faba | |
| 8.5 | - |
ADP | non-competitive with respect to galactose | Vicia faba | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vicia faba | isoelectric focusing | - |
5.3 |
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