Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3)-pLysS cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C143S | the mutant retains 75% of wild type enzymatic activity | Rattus norvegicus |
D107A | inactive | Rattus norvegicus |
N36A | the mutation reduces enzyme activity to 51% of the wild type | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ATP | wild type enzyme, at pH 7.5 and 37°C | Rattus norvegicus | |
0.15 | - |
ATP | mutant enzyme C143S, at pH 7.5 and 37°C | Rattus norvegicus | |
0.78 | - |
N-acetyl-D-glucosamine | wild type enzyme, at pH 7.5 and 37°C | Rattus norvegicus | |
0.86 | - |
N-acetyl-D-glucosamine | mutant enzyme C143S, at pH 7.5 and 37°C | Rattus norvegicus | |
1.17 | - |
N-acetyl-D-glucosamine | mutant enzyme N36A, at pH 7.5 and 37°C | Rattus norvegicus | |
1.59 | - |
ATP | mutant enzyme N36A, at pH 7.5 and 37°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Rattus norvegicus | - |
ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hippocampus | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
GlcNAc kinase | - |
Rattus norvegicus |
N-acetyl-D-glucosamine kinase | - |
Rattus norvegicus |
NagK | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | the small domain of the enzyme, and not its kinase activity, plays a critical role in the upregulation of dendritogenesis | Rattus norvegicus |