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Information on EC 2.7.1.59 - N-acetylglucosamine kinase
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2.7.1.59 N-acetylglucosamine kinaseIUBMB Comments
The bacterial enzyme also acts on D-glucose.
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Word Map
- 2.7.1.59
- albicans
- n-acetylmannosamine
- hexokinases
-
glcnac-6-phosphate
- glucosamine-6-phosphate
-
aminosugars
- udp-n-acetylglucosamine
- udp-glcnac
-
dynlrb1
-
n-acetylneuraminic
- dynein
-
roadblock
- mannac
-
2-epimerase
- analysis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
n-acetylglucosamine kinase, glcnac kinase, n-acetyl-d-glucosamine kinase, canag5p, glcnac-kinase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-acetylamino-2-deoxy-D-glucose kinase
-
-
-
-
acetylaminodeoxyglucokinase
-
-
-
-
acetylglucosamine kinase(phosphorylating)
-
-
-
-
ATP:2-acetylamino-2-deoxy-D-glucose 6-phosphotransferase
-
-
-
-
CaNag5p
GlcNAc kinase
GlcNAc-kinase
Hxk1
N-acetyl-D-glucosamine kinase
N-acetylglucosamine kinase
NAG5
NAGA kinase
NagK
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ATP:N-acetyl-D-glucosamine 6-phosphotransferase
The bacterial enzyme also acts on D-glucose.
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CAS REGISTRY NUMBER
COMMENTARY
9027-48-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-D-galactosamine 6-phosphate
-
-
-
-
?
CTP + N-acetyl-D-glucosamine
CDP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
dATP + N-acetyl-D-glucosamine
dADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
GTP + N-acetyl-D-glucosamine
GDP + N-acetyl-D-glucosamine 6-phosphate
-
30% of the activity with ATP
-
-
?
ITP + N-acetyl-D-glucosamine
IDP + N-acetyl-D-glucosamine 6-phosphate
-
75% of the activity with ATP
-
-
?
N-acetylglucosamine + ATP
N-acetylglucosamine 6-phosphate + ADP
-
predominantely beta-N-acetylglucosamine 6-phosphate
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
15% of the rate with N-acetyl-D-glucosamine
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
21% of the activity with N-acetyl-D-glucosamine
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
very poor substrate, 4.5% of the rate with N-acetyl-D-glucosamine
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
at equal rate than N-acetyl-D-glucosamine
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
at equal rate than N-acetyl-D-glucosamine
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
14% activity compared to N-acetyl-D-glucosamine
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
14% activity compared to N-acetyl-D-glucosamine
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
-
5% activity compared to N-acetyl-D-glucosamine
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
-
5% activity compared to N-acetyl-D-glucosamine
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
highly specific for this substrate
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
Q59RG5
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
highly specific
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
first reaction of inducible N-acetylglucosamine catabolic pathway, enables organism to grow on N-acetylglucosamine as sole carbon source
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
first reaction of inducible N-acetylglucosamine catabolic pathway, enables organism to grow on N-acetylglucosamine as sole carbon source
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
part of glycoprotein synthesis pathway
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
first enzyme of N-acetyl-D-glucosamine salvage pathway
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
highly specific
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
involved in UDP-N-acetylglucosamine formation
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
100% activity
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
100% activity
-
-
?
ATP + N-acetyl-D-mannosamine
ADP + N-acetyl-D-mannosamine 6-phosphate
-
roughly 50% of activity
-
-
?
ATP + N-acetyl-D-mannosamine
ADP + N-acetyl-D-mannosamine 6-phosphate
recombinant enzyme, same rate as with N-acetyl-D-glucosamine
-
-
?
ATP + N-acetyl-D-mannosamine
ADP + N-acetyl-D-mannosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-mannosamine
ADP + N-acetyl-D-mannosamine 6-phosphate
-
roughly 50% of activity
-
-
?
ATP + N-acetyl-D-mannosamine
ADP + N-acetyl-D-mannosamine 6-phosphate
-
78% of the rate with N-acetyl-D-glucosamine
-
-
?
additional information
?
-
-
no activity with N-acetyl-D-mannose, D-mannose, D-galactose, and D-glucosamine
-
-
?
additional information
?
-
-
D-fructose, D-galactose, galactosamine, N-acetyl-D-galactosamine, mannosamine, N-acetyl-D-mannosamine do not serve as substrates
-
-
?
additional information
?
-
-
interaction of HXK1 with histone deacetylase SIR2 under filamentation inducing conditions
-
-
?
additional information
?
-
-
the enzyme has a unique specificity for both amino sugars of the bacterial cell wall, N-acetylmuramic acid and N-acetylglucosamine, which are phosphorylated at the 6-hydroxyl group, see also EC 2.7.1.170, the kcat value is 1.5fold higher for the latter substrate. Neither the non-N-acetylated forms of the cell wall sugars, i.e., glucosamine and/or muramic acid, nor epimeric hexoses or 1,6-anhydro-MurNAc are substrates for the enzyme. Product identificsation by nonradioactive and radioactive TLC
-
-
?
additional information
?
-
-
N-methyl-D-glucosamine, D-glucosamine, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates, very low activities with UTP, GTP and CTP
-
-
?
additional information
?
-
-
poor activity with mannose, galactose, glucosamine, D-mannosamine, N-acetylmannosamine, and N-acetylgalactosamine
-
-
?
additional information
?
-
-
D-fructose, D-galactose, galactosamine, N-acetyl-D-galactosamine, mannosamine, N-acetyl-D-mannosamine do not serve as substrates
-
-
?
additional information
?
-
D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, ribose, fucose do not serve as substrates
-
-
?
additional information
?
-
-
D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, ribose, fucose do not serve as substrates
-
-
?
additional information
?
-
-
no activity with D-galactose and D-fructose, very low activities with N-acetyl-D-galactosamine: 7.6%, D-glucosamine: 11.2%, D-mannosamine: 2.7%, D-galactosamine: 2.1%, D-mannose: 4.5% and D-xylose: 2.1%
-
-
?
additional information
?
-
-
D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates
-
-
?
additional information
?
-
-
D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates
-
-
?
additional information
?
-
-
D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates
-
-
?
additional information
?
-
-
no activity with UTP, CTP, ADP or phosphoenol pyruvate
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
Q59RG5
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
first reaction of inducible N-acetylglucosamine catabolic pathway, enables organism to grow on N-acetylglucosamine as sole carbon source
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
first reaction of inducible N-acetylglucosamine catabolic pathway, enables organism to grow on N-acetylglucosamine as sole carbon source
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
part of glycoprotein synthesis pathway
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
first enzyme of N-acetyl-D-glucosamine salvage pathway
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
involved in UDP-N-acetylglucosamine formation
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
100% activity
-
-
?
ATP + N-acetyl-D-glucosamine
ADP + N-acetyl-D-glucosamine 6-phosphate
-
100% activity
-
-
?
additional information
?
-
-
interaction of HXK1 with histone deacetylase SIR2 under filamentation inducing conditions
-
-
?
additional information
?
-
-
the enzyme has a unique specificity for both amino sugars of the bacterial cell wall, N-acetylmuramic acid and N-acetylglucosamine, which are phosphorylated at the 6-hydroxyl group, see also EC 2.7.1.170, the kcat value is 1.5fold higher for the latter substrate. Neither the non-N-acetylated forms of the cell wall sugars, i.e., glucosamine and/or muramic acid, nor epimeric hexoses or 1,6-anhydro-MurNAc are substrates for the enzyme. Product identificsation by nonradioactive and radioactive TLC
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
Mg2+
Mn2+
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
-
substrates partially protect against inactivation
arsenite/2,3-dimercaptopropanol
-
substrates partially protect against inactivation
EDTA
-
a 3fold excess of EDTA compared to the Mg2+ concentration strongly represses the MurK activity. Addition of 2 mM MgCl2 to the reaction mixture partially restores MurK activity
p-hydroxymercuribenzoate
-
no protection by substrate, protection but not reversal of inhibition by thioethanol, complete inhibition above 0.00125 mM, cysteine reverses inhibition
N-acetyl-D-glucosamine
inhibition of activity with N-acetyl-D-mannosamine
N-acetyl-D-mannosamine
no inhibition of activity with N-acetyl-D-glucosamine
p-chloromercuribenzoate
-
complete inhibition at 0.4 mM; reversible with cysteine or 2-mercaptoethanol
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2- mercaptoethanol
-
reverses inhibition with p-chloromercuribenzoate and loss of activity during storage
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Candidiasis
Attenuation of virulence and changes in morphology in Candida albicans by disruption of the N-acetylglucosamine catabolic pathway.
Liver Cirrhosis, Biliary
Hexokinase isoenzymes in normal and cirrhotic human liver: suppression of glucokinase in cirrhosis.
Liver Diseases, Alcoholic
Hexokinase isoenzymes in normal and cirrhotic human liver: suppression of glucokinase in cirrhosis.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information<