Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in strain XL1-Blue | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutants lack enzyme activity in the cytoplasm, but do not accumulate N-acetyl-D-glucosamine suggesting the existence of an alternative pathway for reutilization of N-acetyl-D-glucosamine, overview | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ZnSO4 | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
N-acetyl-D-glucosamine | pH 7.8, 37°C | Escherichia coli | |
0.9 | - |
ATP | pH 7.8, 37°C | Escherichia coli | |
37.2 | - |
D-glucose | pH 7.8, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Escherichia coli | enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene nagK | - |
Purification (Comment) | Organism |
---|---|
native enzyme 842fold from K12 strain MG1655 in a multistep procedure, recombinant enzyme from strain XL1-Blue | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0183 | - |
wild-type cell extract | Escherichia coli |
33.3 | - |
purified native enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, purified recombinant enzyme frozen in dry-ice/ethanol, fully stable for months, thawed samples retain full activity in 40% glycerol at -20°C for a few months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | 21% of the activity with N-acetyl-D-glucosamine | Escherichia coli | ADP + D-glucose 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | - |
Escherichia coli | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded | Escherichia coli | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
additional information | poor activity with mannose, galactose, glucosamine, D-mannosamine, N-acetylmannosamine, and N-acetylgalactosamine | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acetyl-D-glucosamine kinase | - |
Escherichia coli |
NagK | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |