BRENDA - Enzyme Database show
show all sequences of 2.7.1.49

Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase

Park, J.H.; Burns, K.; Kinsland, C.; Begley, T.P.; J. Bacteriol. 186, 1571-1573 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene annotated ThiD, instead of YjbV, subcloning in Escherichia coli strain DH5alpha, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
MgCl2 at 2 mM
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
Bacillus subtilis
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
Bacillus subtilis
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
?
additional information
Bacillus subtilis
the enzyme has both a biosynthetic and a salvage pathway function
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
gene annotated ThiD, instead of YjbV
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
662007
Bacillus subtilis
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
662007
Bacillus subtilis
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
-
?
additional information
the enzyme has both a biosynthetic and a salvage pathway function
662007
Bacillus subtilis
?
-
-
-
-
additional information
no activity with pyridoxal, pyridoxine, or pyridoxamine
662007
Bacillus subtilis
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene annotated ThiD, instead of YjbV, subcloning in Escherichia coli strain DH5alpha, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
MgCl2 at 2 mM
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
Bacillus subtilis
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
Bacillus subtilis
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
?
additional information
Bacillus subtilis
the enzyme has both a biosynthetic and a salvage pathway function
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
662007
Bacillus subtilis
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
662007
Bacillus subtilis
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
-
?
additional information
the enzyme has both a biosynthetic and a salvage pathway function
662007
Bacillus subtilis
?
-
-
-
-
additional information
no activity with pyridoxal, pyridoxine, or pyridoxamine
662007
Bacillus subtilis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
Other publictions for EC 2.7.1.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738234
Castro-Fernandez
Emergence of pyridoxal phospho ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
FEBS Lett.
588
3068-3073
2014
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2
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-
1
1
-
-
-
738451
Nodwell
A subfamily of bacterial ribok ...
Staphylococcus aureus, Staphylococcus aureus ATCC 700699
J. Am. Chem. Soc.
136
4992-4999
2014
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-
1
1
2
-
1
6
-
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2
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4
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6
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1
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1
2
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1
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6
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4
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6
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722048
Nodwell
Rugulactone and its analogues ...
Staphylococcus aureus
ChemBioChem
13
1439-1446
2012
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3
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1
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3
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3
3
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718477
French
Structure of trifunctional THI ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. D
67
784-791
2011
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-
1
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2
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1
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686824
Onozuka
Involvement of thiaminase II e ...
Saccharomyces cerevisiae
FEMS Yeast Res.
8
266-275
2008
-
-
1
-
-
-
-
-
-
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1
-
1
-
-
1
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2
-
1
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1
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1
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1
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1
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2
-
1
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-
1
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-
671903
Rapala-Kozik
Molecular characterization of ...
Zea mays
Biochem. J.
408
149-159
2007
-
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1
-
7
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3
-
-
5
-
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1
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-
1
-
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1
1
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1
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1
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1
1
-
7
-
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3
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1
-
1
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1
1
-
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1
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-
672477
Wrenger
Vitamin B1 de novo synthesis i ...
Plasmodium falciparum
Biol. Chem.
387
41-51
2006
-
-
1
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-
-
-
2
-
-
1
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1
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1
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2
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4
1
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1
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1
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2
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1
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1
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2
-
4
1
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-
1
-
-
-
-
-
-
-
-
-
-
674302
Karunakaran
Thiamine is synthesized by a s ...
Rhizobium leguminosarum
J. Bacteriol.
188
6661-6668
2006
-
-
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-
-
-
-
-
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1
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1
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1
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1
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1
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-
661316
Haas
Thi20, a remarkable enzyme fro ...
Saccharomyces cerevisiae
Bioorg. Chem.
33
338-344
2005
-
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1
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1
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3
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
661537
Kawasaki
Biosynthesis of hydroxymethylp ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae YPH500
Curr. Genet.
47
156-162
2005
-
-
1
-
1
-
-
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1
1
4
-
4
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1
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1
-
6
1
1
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1
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1
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1
1
-
1
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1
1
4
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1
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1
-
6
1
1
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1
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-
662007
Park
Characterization of two kinase ...
Bacillus subtilis
J. Bacteriol.
186
1571-1573
2004
-
-
1
-
-
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-
1
-
1
-
3
-
2
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1
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4
-
1
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1
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1
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1
1
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1
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1
-
3
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1
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-
4
-
1
-
-
-
1
-
-
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-
641661
Mizote
Cloning and characterization o ...
Escherichia coli, Escherichia coli BL21(DE3) (pLysS)
Microbiology
145
495-501
1999
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1
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2
2
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4
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1
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1
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6
1
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2
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2
2
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1
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1
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6
1
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639726
Kim
A Brassica cDNA clone encoding ...
Arabidopsis thaliana, Brassica napus
Plant Mol. Biol.
37
955-966
1998
-
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2
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2
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2
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2
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3
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3
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641660
Reddick
-
Overexpression, purification a ...
Escherichia coli
Tetrahedron
54
15983-15991
1998
-
1
1
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3
-
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3
1
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1
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1
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1
4
1
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3
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1
1
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3
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1
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1
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4
1
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3
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641658
Mizote
Purification and properties of ...
Escherichia coli
Biochim. Biophys. Acta
991
109-113
1989
-
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2
4
1
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1
1
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2
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1
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1
1
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5
1
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2
2
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2
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2
2
4
1
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1
1
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1
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1
1
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5
1
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2
2
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641657
Lewin
-
The biosynthesis of thiamine. ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
2768-2771
1961
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1
1
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1
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1
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6
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1
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1
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1
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6
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