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Literature summary for 2.7.1.23 extracted from

  • Lindner, S.N.; Niederholtmeyer, H.; Schmitz, K.; Schoberth, S.M.; Wendisch, V.F.
    Polyphosphate/ATP-dependent NAD kinase of Corynebacterium glutamicum: biochemical properties and impact of ppnK overexpression on lysine production (2010), Appl. Microbiol. Biotechnol., 87, 583-593.
    View publication on PubMed
Search for more literature for 2.7.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ ATP-dependent activity is reduced to 72% in the presence of 20 mM Ca2+ Corynebacterium glutamicum
KCl 200 mM KCl decreases the polyphosphate-dependent activity to 75% Corynebacterium glutamicum
NaCl 200 mM NaCl decreases the polyphosphate-dependent activity to 75% Corynebacterium glutamicum
NADH
-
 Corynebacterium glutamicum
NADP+
-
 Corynebacterium glutamicum
Ni2+ ATP-dependent activity is reduced to 60% or 7% in the presence of 5 or 20 mM Ni2+ Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
 ATP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
0.67
-
 NAD+ in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
1.1
-
 NAD+ in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.1
-
 Tetraphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.4
-
 Triphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
3.1
-
 polyphosphate(75) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
4.3
-
 ADP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
5.9
-
 polyphosphate(20) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
14.1
-
 polyphosphate(45) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
17.2
-
 polyphosphate(25) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
18.4
-
 polyphosphate(65) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme activity is enhanced by Mg2+. The highest activity for ATP is reached with 20 mM Mg2+ (2.6fold higher than with ATP, but without addition of bivalent cations) Corynebacterium glutamicum
Mn2+ enzyme activity is enhanced by Mn2+. When using polyphosphate, the enzyme requires bivalent cations, preferably manganese ions, for activity. With polyphosphate, the enzyme is most active in the presence of 15 mM Mn2+ (60% activity compared to ATP with 20 mM Mg2+). 20 mM Mn2+ increases ATP-dependent activity 1.9fold Corynebacterium glutamicum
additional information addition of NaCl or KCl does not alter ATP-dependent activity Corynebacterium glutamicum
additional information enzyme activity is independent of bivalent cations when using ATP Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34300
-
 2 * 34300, SDS-PAGE Corynebacterium glutamicum
167500
-
 gel filtration Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum ATCC 13032
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + NAD+
-
 Corynebacterium glutamicum AMP + NADP+
-
 ?
ADP + NAD+
-
 Corynebacterium glutamicum ATCC 13032 AMP + NADP+
-
 ?
ATP + NAD+ the catalytic efficiency with ATP as phosphate donor for phosphorylation of NAD is higher than with polyphosphate. The enzyme prefers ATP over ADP and polyphosphate(20) Corynebacterium glutamicum ADP + NADP+
-
 ?
ATP + NAD+ the catalytic efficiency with ATP as phosphate donor for phosphorylation of NAD is higher than with polyphosphate. The enzyme prefers ATP over ADP and polyphosphate(20) Corynebacterium glutamicum ATCC 13032 ADP + NADP+
-
 ?
polyphosphate(20) + NAD+
-
 Corynebacterium glutamicum polyphosphate(19) + NADP+
-
 ?
polyphosphate(20) + NAD+
-
 Corynebacterium glutamicum ATCC 13032 polyphosphate(19) + NADP+
-
 ?
polyphosphate(25) + NAD+
-
 Corynebacterium glutamicum polyphosphate(24) + NADP+
-
 ?
polyphosphate(25) + NAD+
-
 Corynebacterium glutamicum ATCC 13032 polyphosphate(24) + NADP+
-
 ?
polyphosphate(45) + NAD+
-
 Corynebacterium glutamicum polyphosphate(44) + NADP+
-
 ?
polyphosphate(45) + NAD+
-
 Corynebacterium glutamicum ATCC 13032 polyphosphate(44) + NADP+
-
 ?
polyphosphate(65) + NAD+
-
 Corynebacterium glutamicum polyphosphate(64) + NADP+
-
 ?
polyphosphate(75) + NAD+
-
 Corynebacterium glutamicum polyphosphate(74) + NADP+
-
 ?
tetraphosphate + NAD+
-
 Corynebacterium glutamicum triphosphate + NADP+
-
 ?
triphosphate + NAD+
-
 Corynebacterium glutamicum diphosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 2 * 34300, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
polyP/ATP-dependent NAD kinase
-
 Corynebacterium glutamicum
Ppnk
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 65
-
 Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 60 preheating the enzyme for 1 h at temperatures up to 45°C does not alter the activity. When preheated at 50°C for 1 h, enzyme activity measured at 30°C decreases by 30% and preincubation at 56°C and 60°C leads to a fast and complete loss of activity after 10 and 5 min, respectively Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 polyphosphate(75) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.3
-
 ADP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
3
-
 Triphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
3.4
-
 polyphosphate(45) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
3.4
-
 polyphosphate(65) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
7.3
-
 polyphosphate(25) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
7.8
-
 polyphosphate(20) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
7.8
-
 NAD+ in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
8.7
-
 Tetraphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
27.1
-
 NAD+ in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
29.2
-
 ATP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 for polyphosphate-dependent activity Corynebacterium glutamicum
7.3
-
 for ATP-dependent activity Corynebacterium glutamicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.11
-
 NADH in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
0.34
-
 NADP+ in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 polyphosphate(65) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
0.24
-
 polyphosphate(45) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
0.42
-
 polyphosphate(25) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
0.5
-
 ADP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
0.58
-
 polyphosphate(75) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
1.2
-
 Triphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
1.3
-
 polyphosphate(20) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
4.1
-
 Tetraphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
7.05
-
 NAD+ in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
40.5
-
 NAD+ in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
59.6
-
 ATP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum