Information on EC 2.7.1.23 - NAD+ kinase

physiological function

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ADP + NAD+

AMP + NADP+

ADP + NADH

AMP + NADPH

C4LSN1;

13% activity compared to ATP

ATP + 3-acetyl pyridineadenine dinucleotide

ATP + NAD+

ADP + NADP+

74 entries

ATP + NADH

ADP + NADPH

19 entries

CTP + NAD+

CDP + NADP+

CTP + NADH

CDP + NADPH

dATP + NAD+

dADP + NADP+

8 entries

dATP + NADH

dADP + NADPH

C4LSN1;

103% activity compared to ATP

dCTP + NAD+

dCDP + NADP+

dGTP + NAD+

dGDP + NADP+

di-adenosine diphosphate

diphosphate + NAD+

phosphate + NADP+

10% of the activity with ATP

dITP + NAD+

dIDP + NADP+

141% activity compared to ATP

dTTP + NAD+

dTDP + NADP+

35% of the activity with ATP

fructose-1,6-bisphosphate + ?

GTP + NAD+

GDP + NADP+

GTP + NADH

GDP + NADPH

C4LSN1;

102% activity compared to ATP

hexametaphosphate + NAD+

? + NADP+

194% activity compared to ATP

hexametaphosphate + NAD+

NADP+ + ?

47% of the activity with ATP

hexaphosphate + NAD+

pentaphosphate + NADP+

hexapolyphosphate + NAD+

pentapolyphosphate + NADP+

ITP + NAD+

IDP + NADP+

metaphosphate + NAD+

? + NADP+

280% activity compared to ATP

NAD+ + ATP

NADP+ + ADP

NAD+ + poly(P)4

NADP+ + poly(P)3

NADH + ATP

NADPH + ADP

661696

NADH + poly(P)4

NADPH + poly(P)3

polyphosphate + NAD+

(phosphate)n-1 + NADP+

polyphosphate + NAD+

polyphosphate(20) + NAD+

polyphosphate(19) + NADP+

polyphosphate(25) + NAD+

polyphosphate(24) + NADP+

polyphosphate(45) + NAD+

polyphosphate(44) + NADP+

polyphosphate(65) + NAD+

polyphosphate(64) + NADP+

polyphosphate(75) + NAD+

polyphosphate(74) + NADP+

tetraphosphate + NAD+

triphosphate + NADP+

tetrapolyphosphate + NAD+

tripolyphosphate + NADP+

84% activity compared to ATP

triphosphate + NAD+

diphosphate + NADP+

TTP + NAD+

TDP + NADP+

TTP + NADH

TDP + NADPH

C4LSN1;

50% activity compared to ATP

UTP + NAD+

UDP + NADP+

10 entries

UTP + NADH

UDP + NADPH

additional information

17 entries

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ATP + NAD+

ADP + NADP+

12 entries

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NAD+

22 entries

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

Ca2+

CaCl2

Co2+

CoCl2

Q6L7J5;

31% of activity with MgCl2

663300

Cu2+

CuCl2

Pyrococcus horikoshii

5 mM, substrate polyP27, 100% activity, substrate ATP, 62% activity

Fe2+

Mg2+

MgCl2

Mn2+

MnCl2

Ni2+

C4LSN1;

6% activity at 5 mM compared to Mg2+

NiCl2

Pyrococcus horikoshii

5 mM, substrate polyP27, 80% activity, substrate ATP, 36% activity

660762

Zn2+

ZnCl2

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

(2S,3R)-5-hydroxy-6,8,10-trimethoxy-2,3-dimethyl-2,3-dihydro-4H-naphtho-(2,3-b)-pyran-4-one

phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0243 mM

(2S,3R)-5-hydroxy-6,8-dimethoxy-2,3-dimethyl-2,3-dihydro-4H-naphtho-(2,3-b)-pyran-4-one

phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0422 mM

(2S,3S)-5-hydroxy-6,8,10-trimethoxy-2,3-dimethyl-4H-2,3-dihydronaphtho-(2,3-b)-pyran-4-one

phytotoxin from Guanomyces polythrix, 50% inhibition at 0.022 mM

(2S,3S)-5-hydroxy-6,8-dimethoxy-2,3-dimethyl-4H-2,3-dihydronaphtho-(2,3-b)-pyran-4-one

phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0401 mM

2'-AMP

2 mM, 25% inhibition

2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide

Q8Y8D7;

723781

3',5'-cAMP

3.5 mM, 20% inhibition

3'-AMP

2 mM, 20% inhibition

3-(bromoacetyl)pyridine

3-acetyl-pyridine adenine dinucleotide

1 mM, 30% inhibition

3-acetylpyridine-adenine-dinucleotide (oxidized form)

0.5 mM, 10% inhibition

3-aldehyde-pyridine adenine dinucleotide

1 mM, 32% inhibition

5'-AMP

2 mM, 15% inhibition

5'-thioacetyladenosine

5,5'-dithiobis(2-nitrobenzoate)

Euglena gracilis

0.5 mM, 62% inhibition of isoenzyme 1 and 33% inhibition of isoenzyme 2

641192

5-hydroxy-6,8-dimethoxy-2,3-dimethyl-4H-naphtho-(2,3-b)-pyran-4-one

phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0171 mM

adenosine diphosphoribose

3.5 mM, 31% inhibition

ADP

AMP

3.5 mM, 29% inhibition

Ca2+

CH3HgBr

0.1 mM, 63% inhibition

chlorpromazin

5 mM, 11% inhibition of the 410000 Da isoform, 80% inhibition of the 63000 Da isoform

citrate

3.5 mM, 55% inhibition

citrinin

phytotoxin from Guanomyces polythrix, 50% inhibition above 0.120 mM

deaminopyridinealdehyde adenine dinucleotide

0.5 mM, 10% inhibition

di-(5'-thioadenosine)

diphosphate

5 mM, 46% inhibition of the 410000 Da isoform, 69% inhibition of the 63000 Da isoform

dithiothreitol

Euglena gracilis

0.1 mM, 81% inhibition, irreversible for isoenzyme 1 and reversible for isoenzyme 2

641192

DTNB

0.1 mM, 74% inhibition

EGTA

5 mM, 20% inhibition of the 410000 Da isoform, 85% inhibition of the 63000 Da isoform

ergosta-4,6,8(14),22-tetraen-3-one

phytotoxin from Guanomyces polythrix, 50% inhibition at 0.090 mM

Hg(CH3COO)2

0.1 mM, complete inhibition

HgCl2

iodoacetic acid

1 mM, 56% inhibition of the 63000 Da isoform, no inhibition of the 410000 Da isoform

KCl

200 mM KCl decreases the polyphosphate-dependent activity to 75%

Mn2+

above 1 mM

NaCl

200 mM NaCl decreases the polyphosphate-dependent activity to 75%

NAD+

inhibits exchange reaction between ADP and ATP

641184

NADH

NADP

NADP+

NADPH

NEM

1 mM, 92% inhibition of the 63000 Da isoform, 34% inhibition of the 410000 Da isoform

Ni2+

ATP-dependent activity is reduced to 60% or 7% in the presence of 5 or 20 mM Ni2+

nicotinamide guanine dinucleotide

1 mM, 23% inhibition

nicotinamide hypoxanthine dinucleotide

1 mM, 24% inhibition

nicotinamide mononucleotide

2 mM, 15% inhibition

nicotinic acid adenine dinucleotide phosphate

1 mM, 37% inhibition

PCMB

phosphoenolpyruvate

3.5 mM, 20% inhibition

pyruvate

3.5 mM, 10% inhibition

R24571

1 mM, 46% inhibition of the 410000 Da isoform, 89% inhibition of the 63000 da isoform

rubrofusarin B

phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0133 mM

shRNA

shRNA1-NADK exhibits a notably higher efficiency compared with a shRNA2-NADK construct. About 70% decrease of both NADK expression, activity, and the NADPH concentration, accompanied by increased sensitivity toward H2O2

Trifluoperazine

W-7

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

5'-AMP

1.56fold activation at 3.0 mM

adenine

5 mM, activity of both isoenzymes is modestly enhanced

AMP

5 mM, activity of both isoenzymes is modestly enhanced

Ca2+

Caffeine

5 mM, activity of both isoenzymes is modestly enhanced

Calmodulin

14 entries

D-ribose 5-phosphate

5 mM, activity of both isoenzymes is modestly enhanced

H2O2

Q500Y9;, Q56YN3;, Q9C5W3;

upregulates NADK1 by 3fold after treatment of plant cells with 5 mM

imidazole

5 mM, activity of both isoenzymes is modestly enhanced

nicotinic acid

5 mM, activity of both isoenzymes is modestly enhanced

Pyridine

5 mM, activity of both isoenzymes is modestly enhanced

quinolinic acid

allosteric activator

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

4.3

ADP

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.032 - 4.5

ATP

32 entries

0.59

CTP

pH 8

0.43

fructose-1,6-bisphosphate

2.86

GTP

pH 8

1.54

ITP

pH 8

0.08 - 0.74

MgATP2-

0.2

MgGTP2-

Euglena gracilis

isoenzyme 2

641192

0.11 - 6.5

NAD+

43 entries

0.05 - 2

NADH

0.18

NADP+

0.3

NADPH

0.21 - 0.33

poly(P)4

2.2 - 2.7

Polyphosphate

5.9

polyphosphate(20)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

17.2

polyphosphate(25)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

14.1

polyphosphate(45)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

18.4

polyphosphate(65)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

3.1

polyphosphate(75)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

2.1

Tetraphosphate

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

Tetrapolyphosphate

in 100 mM Tris-HCl (pH 8.0), at 37°C

2.4

Triphosphate

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.42

UTP

pH 8

additional information

allosteric enzyme, Hill coefficient for ATP 1.5, for polyphosphate 1.4

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

2.3

ADP

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.52 - 399

ATP

212

fructose-1,6-bisphosphate

0.41 - 424

NAD+

15 entries

1.9

NADH

C4LSN1;

in 100 mM Tris-HCl (pH 7.5), at 37°C

212

NADP+

236

NADPH

7.8

polyphosphate(20)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

7.3

polyphosphate(25)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

3.4

polyphosphate(45), polyphosphate(65)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

1.8

polyphosphate(75)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

8.7

Tetraphosphate

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

Triphosphate

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

additional information

the molecular activity is 360 per min, the activity per active centre is less than 60 per min, pH 7.4, 30°C

641182

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.5

ADP

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.24 - 59.6

ATP

7 entries

0.36 - 40.5

NAD+

7 entries

56.48

NADH

C4LSN1;

in 100 mM Tris-HCl (pH 7.5), at 37°C

1.3

polyphosphate(20)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.42

polyphosphate(25)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.24

polyphosphate(45)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.19

polyphosphate(65)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

0.58

polyphosphate(75)

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

4.1

Tetraphosphate

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

1.2

Triphosphate

in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.025

2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide

Q8Y8D7;

in 50 mM Tris-HCl, pH 7.4, 10 mM MgCl2, at 30°C

723781

3.6

5'-thioacetyladenosine

0.02

di-(5'-thioadenosine)

0.03 - 1.02

NADH

0.04

NADP

0.13 - 0.43

NADP+

0.013 - 0.22

NADPH

2.8

Trifluoperazine

pH 8

0.01

W-7

pH 8

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.0009966

0.093

641179

0.222

0.233

0.281

Sphingomonas sp.

Q6L7J5;

30°C, pH 8.0

663300

0.5

substrates ATP, NAD+, mutant G208A, 37°C, pH 8.0

0.7

substrates ATP, NAD+, mutant S199A, 37°C, pH 8.0

0.8

641182

0.94

Bacillus licheniformis

641183

1.1

substrates ATP, NAD+, mutant T195A, 37°C, pH 8.0

1.2

substrates ATP, NAD+, wild-type, 37°C, pH 8.0

1.4

pH 7.0, 37°C, substrate NAD+, cosubstrate poly(P)4, mutant G183R

1.5

1.78

pH 7.0, 37°C, substrate NAD+, cosubstrate ATP, mutant G183R

2.2

substrates polyphosphate, NAD+, mutant G208A, 37°C, pH 8.0

2.33

pH 7.0, 37°C, mutant R175G, substrate NAD+

2.5

substrates polyphosphate, NAD+, mutant S199A, 37°C, pH 8.0

2.68

pH 7.0, 37°C, mutant R175, substrate ATP

3.2

641185

3.5

substrates polyphosphate, NAD+, mutant T195A, 37°C, pH 8.0

3.8

substrates polyphosphate, NAD+, wild-type, 37°C, pH 8.0

3.84

Solanum pimpinellifolium

activated by Ca2+-calmodulin

6.7

8.63

pH 7.0, 37°C, substrate NAD+, cosubstrate poly(P)4, wild-type

11.1

isoform NADK1, pH 7.9, 25°C; pH 7.9, 25°C

12.45

pH 7.0, 37°C, wild-type, substrate ATP

12.86

pH 7.0, 37°C, wild-type, substrate NAD+

14.3

isoform NADK2, pH 7.9, 25°C; pH 7.9, 25°C

18.67

pH 7.0, 37°C, substrate NAD+, cosubstrate ATP, wild-type

23.2

ATP-dependent activity

641191

24.3

polyphosphate-dependent activity

641191

99.4

213

; calmodulin-depndent isoform, pH 7.9, 25°C

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

6.5

6.8

Bacillus licheniformis

641183

6.8 - 7

Nicotiana tabacum

6.8

Pyrococcus horikoshii

6.8 - 7

7 - 7.6

enzyme from liver

7 - 9.5

Oryctolagus cuniculus

7.1

for polyphosphate-dependent activity

7.2 - 8

7.3

for ATP-dependent activity

7.4 - 7.5

7.5 - 8

410000 Da Ca2+-calmodulin-independent isoform and 63000 Da Ca2+-calmodulin-dependent isoform

7.5

7.6

7.8

7.9

; isoforms NADK1, NADK2

8.5

glycine-NaOH buffer

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

5.5 - 7.5

about 50% of maximal activity at pH 5.5 and pH 7.5

6 - 10

pH 6.0: about 40% of maximal activity, pH 10.0: about 80% of maximal activity

641189

6 - 7

activity decreases sharply below pH 6.0 and above pH 7.0

641179, 641180

6.5 - 7.2

Bacillus licheniformis

90% of maximal activity at pH 6.5 and pH 7.2

641183

6.5 - 9.5

pH 6.5: about 35% of maximal activity, pH 9.5: about 90% of maximal activity

6.8 - 9.2

90% of maximal activity between pH 6.8 and pH 9.2, 410000 Da Ca2+-calmodulin-independent isoform

7 - 10

pH 7.0: about 35% of maximal activity, pH 10.0: about 50% of maximal activity

7.2 - 8.5

90% of maximal activity between pH 7.2 and pH 8.5, 63000 Da Ca2+-calmodulin-dependent isoform

7.9

additional information

broad

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

661696

55 - 65

641189

100

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

25 - 50

25°C: about 45% of maximal activity, 50°C: about 70% of maximal activity

37 - 68

about 50% of maximal activity at 37°C and at 68°C

641189

50 - 55

Sphingomonas sp.

Q6L7J5;

663300

80 - 100

80°C: about 45% of maximal activity, 100°C: optimum

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

5.9

isoelectric focusing

641185

6.5

C4LSN1;

calculated from amino acid sequence

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

maximal activity is observed on day 5 in light-exposed calli or day 14 in day-exposed ones. After 5 d of salt exposure, NAD kinase activity is markedly lower than in control, but NAD kinase of dark-exposed and NaCl-exposed calli is 10times more active than that of both light-exposed and NaCl-exposed calli

641194

cell culture

cell suspension culture

Solanum pimpinellifolium

initiated from white and friable root calli

egg