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Literature summary for 2.7.1.214 extracted from
- ValC, a new type of C7-Cyclitol kinase involved in the biosynthesis of the antifungal agent validamycin A (2007), ChemBioChem, 8, 632-641.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces hygroscopicus subsp. jinggangensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
valienone | pH 7.6, 28°C | Streptomyces hygroscopicus subsp. jinggangensis |  |
| 0.026 | - |
validone | pH 7.6, 28°C | Streptomyces hygroscopicus subsp. jinggangensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36750 | - |
- |
Streptomyces hygroscopicus subsp. jinggangensis |
| 40000 | - |
- |
Streptomyces hygroscopicus subsp. jinggangensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + validone | Streptomyces hygroscopicus subsp. jinggangensis | - |
ADP + validone 7-phosphate | - |
? | |
| ATP + validone | Streptomyces hygroscopicus subsp. jinggangensis 5008 | - |
ADP + validone 7-phosphate | - |
? | |
| ATP + valienone | Streptomyces hygroscopicus subsp. jinggangensis | - |
ADP + valienone 7-phosphate | - |
? | |
| ATP + valienone | Streptomyces hygroscopicus subsp. jinggangensis 5008 | - |
ADP + valienone 7-phosphate | - |
? | |
| additional information | Streptomyces hygroscopicus subsp. jinggangensis | ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives | ? | - |
? | |
| additional information | Streptomyces hygroscopicus subsp. jinggangensis 5008 | ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hygroscopicus subsp. jinggangensis | Q3T6E2 | - |
- |
| Streptomyces hygroscopicus subsp. jinggangensis 5008 | Q3T6E2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + validone | - |
Streptomyces hygroscopicus subsp. jinggangensis | ADP + validone 7-phosphate | - |
? | |
| ATP + validone | - |
Streptomyces hygroscopicus subsp. jinggangensis 5008 | ADP + validone 7-phosphate | - |
? | |
| ATP + valienone | - |
Streptomyces hygroscopicus subsp. jinggangensis | ADP + valienone 7-phosphate | - |
? | |
| ATP + valienone | - |
Streptomyces hygroscopicus subsp. jinggangensis 5008 | ADP + valienone 7-phosphate | - |
? | |
| additional information | ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives | Streptomyces hygroscopicus subsp. jinggangensis | ? | - |
? | |
| additional information | ValC is a C7-cyclitol kinase, which phosphorylates valienone and validone, but not 2-epi-5-epi-valiolone, 5-epi-valiolone, and D-glucose, D-fructose, D-mannose, and D-mannitose, to their 7-phosphate derivatives | Streptomyces hygroscopicus subsp. jinggangensis 5008 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE of recombinant His-tagged protein, x * 36750, calculated | Streptomyces hygroscopicus subsp. jinggangensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| valC | - |
Streptomyces hygroscopicus subsp. jinggangensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Streptomyces hygroscopicus subsp. jinggangensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
valienone | pH 7.6, 28°C | Streptomyces hygroscopicus subsp. jinggangensis | |
| 7.3 | - |
validone | pH 7.6, 28°C | Streptomyces hygroscopicus subsp. jinggangensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Streptomyces hygroscopicus subsp. jinggangensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | inactivation of valC results in mutants that lack the ability to produce validamycin A | Streptomyces hygroscopicus subsp. jinggangensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 180 | - |
valienone | pH 7.6, 28°C | Streptomyces hygroscopicus subsp. jinggangensis | |
| 286 | - |
validone | pH 7.6, 28°C | Streptomyces hygroscopicus subsp. jinggangensis | |
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Release 2023.1 (January 2023)
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