General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Homo sapiens |
very unstable in dilute solution. Addition of albumin to reaction mixture in dilute enzyme preparations increases activity. MgATP2-, 1 mM, adenosine 5 mM, and 20% glycerol stabilize on a short term basis at 4ºC. Repeated freeze-thawing inactivates dilute enzyme solutions | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strong inhibition above 1 mM | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially substitute for Mg2+ | Homo sapiens | |
Co2+ | activation | Homo sapiens | |
Mg2+ | requirement | Homo sapiens | |
Mn2+ | can partially substitute for Mg2+ | Homo sapiens | |
Mn2+ | activation | Homo sapiens | |
Ni2+ | can partially substitute for Mg2+ | Homo sapiens | |
Ni2+ | activation | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37250 | - |
estimated from Stokes radius in polyacrylamide gel electrophoresis | Homo sapiens |
38250 | - |
sucrose density gradient centrifugation | Homo sapiens |
40740 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion exchange and affinity chromatography, partial, 3600fold purification | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
additional information | tissue distribution | Homo sapiens | - |
pancreas | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
spleen | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, 0.5 mg protein/ml or more, purified preparation, at least 8 months | Homo sapiens |
4°C, glycerol, dithiothreitol and adenosine stabilize. 20% glycerol, t1/2 20 days, 75% loss of activity within 60 days. Without glycerol, t1/2 3 days. -70°C to 4°C, 0.01 mg protein/ml, 75 to 95% loss of activity within days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | best substrates | Homo sapiens | ADP + AMP | - |
? | |
GTP + adenosine | - |
Homo sapiens | GDP + AMP | - |
? | |
ITP + adenosine | - |
Homo sapiens | IDP + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38800-47720, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
crude placental supernatant | Homo sapiens |
5.5 | - |
purified preparation, under ATP and adenosine saturating conditions | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
more stable than at pH 5.5 | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | isoelectric focusing | - |
5.9 |