BRENDA - Enzyme Database
show all sequences of 2.7.1.2

Kinetic characteristics and regulatory mechanisms of glucokinase and fructokinase from Zymomonas mobilis

Doelle, H.W.; Eur. J. Appl. Microbiol. Biotechnol. 14, 241-246 (1982)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Zymomonas mobilis
AMP
-
Zymomonas mobilis
CTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
D-fructose 6-phosphate
weak
Zymomonas mobilis
D-glucose 6-phosphate
-
Zymomonas mobilis
GTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
ITP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
TTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
UTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
D-glucose
+ CTP, 30°C, pH 7
Zymomonas mobilis
0.12
-
D-glucose
+ UTP, 30°C, pH 7
Zymomonas mobilis
0.2
-
D-glucose
+ ITP, 30°C, pH 7
Zymomonas mobilis
0.22
-
D-glucose
cosubstrate GTP, 30°C, pH 7
Zymomonas mobilis
0.25
-
GTP
30°C, pH 7
Zymomonas mobilis
0.36
-
ITP
30°C, pH 7
Zymomonas mobilis
0.4
-
ATP
30°C, pH 7
Zymomonas mobilis
0.5
-
D-glucose
+ ATP, 30°C, pH 7
Zymomonas mobilis
0.56
-
CTP
30°C, pH 7
Zymomonas mobilis
0.63
-
UTP
30°C, pH 7
Zymomonas mobilis
0.8
-
ATP
30°C, pH 7
Zymomonas mobilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-glucose
Zymomonas mobilis
-
ADP + D-glucose 6-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zymomonas mobilis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-glucose
-
641057
Zymomonas mobilis
ADP + D-glucose 6-phosphate
-
-
-
?
CTP + D-glucose
-
641057
Zymomonas mobilis
CDP + D-glucose 6-phosphate
-
-
-
?
GTP + D-glucose
-
641057
Zymomonas mobilis
GDP + D-glucose 6-phosphate
-
-
-
?
ITP + D-glucose
-
641057
Zymomonas mobilis
IDP + D-glucose 6-phosphate
-
-
-
?
additional information
enzyme reacts equally well with ATP, UTP, GTP, ITP and CTP
641057
Zymomonas mobilis
?
-
-
-
-
UTP + D-glucose
-
641057
Zymomonas mobilis
UDP + D-glucose 6-phosphate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Zymomonas mobilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
2 optima: a major peak at pH 7.0 and a minor peak at pH 8.2
Zymomonas mobilis
8.2
-
2 optima: a major peak at pH 7.0 and a minor peak at pH 8.2
Zymomonas mobilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Zymomonas mobilis
AMP
-
Zymomonas mobilis
CTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
D-fructose 6-phosphate
weak
Zymomonas mobilis
D-glucose 6-phosphate
-
Zymomonas mobilis
GTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
ITP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
TTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
UTP
above 1 mM, irrespective of glucose concentration
Zymomonas mobilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
D-glucose
+ CTP, 30°C, pH 7
Zymomonas mobilis
0.12
-
D-glucose
+ UTP, 30°C, pH 7
Zymomonas mobilis
0.2
-
D-glucose
+ ITP, 30°C, pH 7
Zymomonas mobilis
0.22
-
D-glucose
cosubstrate GTP, 30°C, pH 7
Zymomonas mobilis
0.25
-
GTP
30°C, pH 7
Zymomonas mobilis
0.36
-
ITP
30°C, pH 7
Zymomonas mobilis
0.4
-
ATP
30°C, pH 7
Zymomonas mobilis
0.5
-
D-glucose
+ ATP, 30°C, pH 7
Zymomonas mobilis
0.56
-
CTP
30°C, pH 7
Zymomonas mobilis
0.63
-
UTP
30°C, pH 7
Zymomonas mobilis
0.8
-
ATP
30°C, pH 7
Zymomonas mobilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-glucose
Zymomonas mobilis
-
ADP + D-glucose 6-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-glucose
-
641057
Zymomonas mobilis
ADP + D-glucose 6-phosphate
-
-
-
?
CTP + D-glucose
-
641057
Zymomonas mobilis
CDP + D-glucose 6-phosphate
-
-
-
?
GTP + D-glucose
-
641057
Zymomonas mobilis
GDP + D-glucose 6-phosphate
-
-
-
?
ITP + D-glucose
-
641057
Zymomonas mobilis
IDP + D-glucose 6-phosphate
-
-
-
?
additional information
enzyme reacts equally well with ATP, UTP, GTP, ITP and CTP
641057
Zymomonas mobilis
?
-
-
-
-
UTP + D-glucose
-
641057
Zymomonas mobilis
UDP + D-glucose 6-phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Zymomonas mobilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
2 optima: a major peak at pH 7.0 and a minor peak at pH 8.2
Zymomonas mobilis
8.2
-
2 optima: a major peak at pH 7.0 and a minor peak at pH 8.2
Zymomonas mobilis
Other publictions for EC 2.7.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737330
Dransfield
Novel series of potent glucoki ...
Homo sapiens
ACS Med. Chem. Lett.
7
714-718
2016
1
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737623
Baldini
Glucokinase expression is regu ...
Mus musculus
Biochem. Biophys. Res. Commun.
478
942-948
2016
-
1
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2
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1
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1
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1
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738083
Ermakova
Structural insight into the gl ...
Homo sapiens
Comput. Biol. Chem.
64
281-296
2016
-
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1
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1
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738698
Markwardt
Regulation of glucokinase by i ...
Mus musculus
J. Biol. Chem.
291
3000-3009
2016
-
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1
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2
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1
1
-
-
-
737605
Hayashi
Roles of hepatic glucokinase i ...
Mus musculus
Biochem. Biophys. Res. Commun.
460
727-732
2015
-
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3
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1
1
-
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738420
Jin
Role of glucokinase in the sub ...
Mus musculus, Rattus norvegicus
Int. J. Mol. Sci.
16
7377-7393
2015
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5
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1
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2
2
-
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738711
Yellapu
Mutations in exons 10 and 11 o ...
Homo sapiens
J. Biomol. Struct. Dyn.
33
820-833
2015
-
1
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15
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2
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1
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15
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738756
Hussain
Glucokinase activity in the ar ...
Rattus norvegicus
J. Clin. Invest.
125
337-349
2015
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2
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1
1
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739028
DAntonio
Structure-based approach to th ...
Trypanosoma cruzi, Trypanosoma cruzi CL Brener
Mol. Biochem. Parasitol.
204
64-76
2015
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1
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3
1
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5
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3
1
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2
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1
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737662
Khu
Regulation of glucokinase acti ...
Urocitellus undulatus
Biochemistry (Moscow)
79
727-732
2014
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1
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1
1
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737954
Lindbloom-Hawley
Cloning and characterization o ...
Felis catus
BMC Vet. Res.
10
130
2014
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738362
Kumar
Comparative structural and fun ...
Staphylococcus aureus, Staphylococcus aureus ATCC 12600
Indian J. Pharm. Sci.
76
430-436
2014
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1
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7
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739603
Martinez
Role of connecting loop I in c ...
Homo sapiens
Protein Sci.
23
915-922
2014
-
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2
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12
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2
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7
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2
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12
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1
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7
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6
6
737327
Bowler
Small-molecule allosteric acti ...
Homo sapiens
ACS Med. Chem. Lett.
4
580-584
2013
3
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737641
Hofmeister-Brix
The ubiquitin-proteasome syste ...
Mus musculus
Biochem. J.
456
173-184
2013
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1
1
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737805
Qian
Thermal stability of glucokina ...
Caldanaerobacter subterraneus subsp. tengcongensis, Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242
BioMed Res. Int.
2013
646539
2013
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1
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3
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4
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11
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1
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1
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1
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3
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11
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1
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1
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1
1
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721535
Bourbonais
Modulation of glucokinase by g ...
Homo sapiens
Biochem. J.
441
881-887
2012
1
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1
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1
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2
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1
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1
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2
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1
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1
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721735
Zelent
Thermal stabilty of glucokinas ...
Homo sapiens
Biochim. Biophys. Acta
1824
769-784
2012
-
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1
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3
2
-
14
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1
3
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1
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1
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1
16
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1
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3
2
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14
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1
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1
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1
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1
16
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721837
Mao
Design, synthesis, and pharmac ...
Homo sapiens
Bioorg. Med. Chem.
20
2982-2991
2012
17
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1
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2
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1
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1
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1
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SAR, pharmacokinetics, safety, ...
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Molecular characterization of ...
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1
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3
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675854
Rui
The Botrytis cinerea hexokinas ...
Botrytis cinerea
Microbiology
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Convergent peripheral pathways ...
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Simultaneous catabolite repres ...
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The crystal structure of Trypa ...
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Caceres
Molecular and biochemical char ...
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2
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Identification and characteriz ...
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1
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1
1
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1
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1
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1
1
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11
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1
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1
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1
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1
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1
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2
1
1
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1
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662836
Lee
Identification of a glucokinas ...
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Mol. Cell
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1
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1
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1
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1
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2
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1
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Biochemical characterization o ...
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3
2
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2
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2
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2
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2
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1
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2
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3
2
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660934
Abdelmoez
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Kinetics of the reaction catal ...
Geobacillus stearothermophilus
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2
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2
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1
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1
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2
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1
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Brocklehurst
Differences in regulatory prop ...
Homo sapiens, Rattus norvegicus
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2004
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2
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2
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2
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2
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2
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2
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2
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2
2
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2
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2
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2
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2
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2
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2
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2
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661139
Miller
Identifying latent enzyme acti ...
Escherichia coli K-12
Biochemistry
43
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1
1
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4
1
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661401
Mesak
Bacillus subtilis GlcK activit ...
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2004
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1
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7
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1
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1
1
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1
2
1
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1
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1
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1
1
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7
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1
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2
1
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662022
Lunin
Crystal structures of Escheric ...
Escherichia coli
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1
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1
1
1
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1
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2
2
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663409
Kamata
Structural basis for allosteri ...
Homo sapiens
Structure
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429-438
2004
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1
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1
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2
1
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1
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1
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1
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1
1
1
1
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1
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1
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1
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1
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1
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2
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Thermostable continuous couple ...
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Gloyn
Insights into the biochemical ...
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9
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661779
Hansen
ATP-dependent glucokinase from ...
Thermotoga maritima
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2003
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5
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1
5
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2
1
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1
1
8
1
1
1
2
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1
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663393
Grimsby
Allosteric activators of gluco ...
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1
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Laht
Cloning and characterization o ...
Aspergillus niger, Saccharomyces cerevisiae, Yarrowia lipolytica
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3
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641072
Roncero
Functional glucokinase isoform ...
Rattus norvegicus
J. Neurochem.
74
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2000
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3
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1
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-
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641063
Tomita
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Synthesis of adenosine 5'-trip ...
Geobacillus stearothermophilus, Zymomonas mobilis
Ann. N. Y. Acad. Sci.
864
548-551
1998
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10
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10
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641065
Meyer
Molecular characterization of ...
Escherichia coli
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Contribution of glucose kinase ...
Bacillus megaterium
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641070
Tu
Glucose regulates the maximal ...
Rattus norvegicus
Diabetes
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1996
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Takeuchi
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Arora
Glucokinase of Escherichia col ...
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Human beta-cell glucokinase. D ...
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Scopes
Kinetic analysis of the activa ...
Zymomonas mobilis
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Klein
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Goward
The purification and character ...
Geobacillus stearothermophilus
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Scopes
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Porter
Purification and kinetic chara ...
Streptococcus mutans
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641055
Porter
-
Glucokinase from Streptococcus ...
Streptococcus mutans
Methods Enzymol.
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Doelle
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Kinetic characteristics and re ...
Zymomonas mobilis
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Hengartner
Isolation and characterization ...
Geobacillus stearothermophilus
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Baumann
Glucokinase of Dictyostelium d ...
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