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6-N-(carboxyethyl)ATP + D-glucose
6-N-(carboxyethyl)ADP + D-glucose 6-phosphate
6-N-(carboxymethyl)ATP + D-glucose
6-N-(carboxymethyl)ADP + D-glucose 6-phosphate
6-N-(succinyl)ATP + D-glucose
6-N-(succinyl)ADP + D-glucose 6-phosphate
6-N-[N-(6-aminohexhyl)carbamoyl]ATP + D-glucose
6-N-[N-(6-aminohexhyl)carbamoyl]ADP + D-glucose 6-phosphate
adenosine 5'-triphosphate-polyamidoamine dendrimer + D-glucose
adenosine 5'-diphosphate-polyamidoamine dendrimer + D-glucose 6-phosphate
-
-
-
ir
ADP + D-glucose
AMP + D-glucose 6-phosphate
ATP + 2-deoxy-D-glucose
ADP + 2-deoxy-D-glucose 6-phosphate
ATP + alpha-D-glucose
ADP + D-glucose 6-phosphate
ATP + beta-D-glucose
ADP + beta-D-glucose 6-phosphate
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
ATP + D-glucosamine
ADP + D-glucosamine 6-phosphate
-
no reaction with the recombinant enzyme
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
ATP + D-mannose
ADP + D-mannose 6-phosphate
ATP + mannoheptulose
?
-
sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence
-
?
ATP + N-acetyl-alpha-D-glucosamine
ADP + N-acetyl-alpha-D-glucosamine 6-phosphate
CTP + D-glucose
ADP + D-glucose 6-phosphate
38% of the velocity with ATP
-
?
CTP + D-glucose
CDP + D-glucose 6-phosphate
D-glucose + ATP
ADP + D-glucose 6-phosphate
-
-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
ITP + D-glucose
IDP + D-glucose 6-phosphate
UTP + D-glucose
UDP + D-glucose 6-phosphate
additional information
?
-
6-N-(carboxyethyl)ATP + D-glucose

6-N-(carboxyethyl)ADP + D-glucose 6-phosphate
-
-
-
?
6-N-(carboxyethyl)ATP + D-glucose
6-N-(carboxyethyl)ADP + D-glucose 6-phosphate
-
-
-
?
6-N-(carboxymethyl)ATP + D-glucose

6-N-(carboxymethyl)ADP + D-glucose 6-phosphate
-
-
-
?
6-N-(carboxymethyl)ATP + D-glucose
6-N-(carboxymethyl)ADP + D-glucose 6-phosphate
-
-
-
?
6-N-(succinyl)ATP + D-glucose

6-N-(succinyl)ADP + D-glucose 6-phosphate
-
-
-
?
6-N-(succinyl)ATP + D-glucose
6-N-(succinyl)ADP + D-glucose 6-phosphate
-
-
-
?
6-N-[N-(6-aminohexhyl)carbamoyl]ATP + D-glucose

6-N-[N-(6-aminohexhyl)carbamoyl]ADP + D-glucose 6-phosphate
-
-
-
?
6-N-[N-(6-aminohexhyl)carbamoyl]ATP + D-glucose
6-N-[N-(6-aminohexhyl)carbamoyl]ADP + D-glucose 6-phosphate
-
-
-
?
ADP + D-glucose

AMP + D-glucose 6-phosphate
3% of the velocity with ATP
-
?
ADP + D-glucose
AMP + D-glucose 6-phosphate
3% of the velocity with ATP
-
?
ATP + 2-deoxy-D-glucose

ADP + 2-deoxy-D-glucose 6-phosphate
-
no reaction with the recombinant enzyme
-
?
ATP + 2-deoxy-D-glucose
ADP + 2-deoxy-D-glucose 6-phosphate
-
-
-
?
ATP + 2-deoxy-D-glucose
ADP + 2-deoxy-D-glucose 6-phosphate
-
sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence
-
?
ATP + 2-deoxy-D-glucose
ADP + 2-deoxy-D-glucose 6-phosphate
-
26% of the activity with D-glucose
-
ir
ATP + alpha-D-glucose

ADP + D-glucose 6-phosphate
-
-
-
?
ATP + alpha-D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-fructose

ADP + D-fructose 6-phosphate
-
no reaction with the recombinant enzyme
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
3% of the velocity with D-glucose
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
3% of the velocity with D-glucose
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
-
-
?
ATP + D-glucose

ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
highly specific for D-glucose
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
specific for D-glucose, substrate binding site structure
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
ir
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
highly specific for D-glucose
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
enzyme plays a key role in whole-body glucose homeostasis
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the pancreatic enzyme is imporatnt as glucose sensor in insulin secretion and blood glucose homeostasis, interaction of ATP and glucose during binding to the enzyme
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
enzyme is only able to phosphorylate glucose
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
ATP is the most efficient phosphoryl donor
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
ATP is the most efficient phosphoryl donor
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the enzyme initiates the intracellular glucose catabolism/metabolism, overview
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the enzyme initiates the intracellular glucose catabolism/metabolism, overview
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
highly specific for D-glucose
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway
-
ir
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the enzyme is specific for D-glucose
-
ir
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
enzyme is only able to phosphorylate glucose
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
highly specific for D-glucose
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
phosphorylates alpha-D-glucose and beta-D-glucose equally fast
-
?
ATP + D-mannose

ADP + D-mannose 6-phosphate
-
no reaction with the recombinant enzyme
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
99% of the velocity with D-glucose
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
99% of the velocity with D-glucose
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
-
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
-
sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
-
-
?
ATP + N-acetyl-alpha-D-glucosamine

ADP + N-acetyl-alpha-D-glucosamine 6-phosphate
-
at pH 7.6: 50% of the activity with alpha-D-glucose, at pH 9.0: 5% of the activity with alpha-D-glucose
-
?
ATP + N-acetyl-alpha-D-glucosamine
ADP + N-acetyl-alpha-D-glucosamine 6-phosphate
-
sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence
-
?
CTP + D-glucose

CDP + D-glucose 6-phosphate
-
10% of the activity with ATP
-
?
CTP + D-glucose
CDP + D-glucose 6-phosphate
-
-
-
?
CTP + D-glucose
CDP + D-glucose 6-phosphate
-
-
-
?
CTP + D-glucose
CDP + D-glucose 6-phosphate
-
less than 10% of the activity with ATP
-
?
CTP + D-glucose
CDP + D-glucose 6-phosphate
-
5% of the activity with ATP as phosphoryl donor
-
?
CTP + D-glucose
CDP + D-glucose 6-phosphate
-
-
-
?
GTP + D-glucose

GDP + D-glucose 6-phosphate
-
no reaction with the recombinant enzyme
-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
-
30% of the activity with ATP
-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
-
less than 10% of ATP
-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
-
12% of the activity with ATP as phosphoryl donor
-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
-
-
-
?
ITP + D-glucose

IDP + D-glucose 6-phosphate
-
-
-
?
ITP + D-glucose
IDP + D-glucose 6-phosphate
-
75% of the activity with ATP
-
?
ITP + D-glucose
IDP + D-glucose 6-phosphate
-
30% of the activity with ATP
-
?
ITP + D-glucose
IDP + D-glucose 6-phosphate
-
30% of the activity with ATP
-
?
ITP + D-glucose
IDP + D-glucose 6-phosphate
-
less than 10% of the activity with ATP
-
?
ITP + D-glucose
IDP + D-glucose 6-phosphate
-
20% of the activity with ATP as phosphoryl donor
-
?
ITP + D-glucose
IDP + D-glucose 6-phosphate
-
-
-
?
UTP + D-glucose

UDP + D-glucose 6-phosphate
-
no reaction with the recombinant enzyme
-
?
UTP + D-glucose
UDP + D-glucose 6-phosphate
-
32% of the activity with ATP
-
?
UTP + D-glucose
UDP + D-glucose 6-phosphate
-
12% of the activity with ATP
-
?
UTP + D-glucose
UDP + D-glucose 6-phosphate
-
less than 10% of the activity with ATP
-
?
UTP + D-glucose
UDP + D-glucose 6-phosphate
-
12% of the activity with ATP as phosphoryl donor
-
?
UTP + D-glucose
UDP + D-glucose 6-phosphate
-
-
-
?
additional information

?
-
hexokinase, Hxk1, but not the glucokinase, Glk1, is required for normal growth and sugar metabolism, and for pathogenicity on fruits
-
?
additional information
?
-
-
hexokinase, Hxk1, but not the glucokinase, Glk1, is required for normal growth and sugar metabolism, and for pathogenicity on fruits
-
?
additional information
?
-
no substrate: N-acetyl-D-glucosamine, D-galactose
-
?
additional information
?
-
-
no substrate: N-acetyl-D-glucosamine, D-galactose
-
?
additional information
?
-
no substrate: N-acetyl-D-glucosamine, D-galactose
-
?
additional information
?
-
-
not: mannose, fructose, 2-deoxyglucose, UTP, ITP, GTP
-
?
additional information
?
-
-
recombinant enzymes NanK and YajF also utilize N-acetyl-D-mannosamine and D-fructose as substrates, respectively
-
?
additional information
?
-
-
loss of enzyme activity is involved in type 2 diabetes mellitus
-
?
additional information
?
-
-
biophysical characterization of the interaction between GK and GKRP and its modulation by physiological and pharmacological effectors
-
?
additional information
?
-
-
less than 10% of the activity with ATP: TTP
-
?
additional information
?
-
-
less than 10% of the activity with ATP: TTP
-
?
additional information
?
-
-
less than 10% of the activity with ATP: TTP
-
?
additional information
?
-
-
less than 10% of the activity with ATP: TTP
-
?
additional information
?
-
comparison of the biochemical properties to those of the enzyme from Streptomyces peucetius var. caesius
-
?
additional information
?
-
-
comparison of the biochemical properties to those of the enzyme from Streptomyces coelicolor
-
?
additional information
?
-
-
no activity with D-mannose and D-fructose as phosphoryl acceptors, no activity with UDP, ADP, acetyl phosphate, and diphosphate as phosphoryl donors
-
?
additional information
?
-
-
no activity with fructose, galactose, and mannose, development of a coupled assay method with glucose 6-phosphate dehydrogenase stable at pH 6.8-8.5 and 75-90°C, overview
-
?
additional information
?
-
-
enzyme reacts equally well with ATP, UTP, GTP, ITP and CTP
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + D-glucose
ADP + D-glucose 6-phosphate
additional information
?
-
ATP + D-glucose

ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
ir
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
enzyme plays a key role in whole-body glucose homeostasis
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the pancreatic enzyme is imporatnt as glucose sensor in insulin secretion and blood glucose homeostasis, interaction of ATP and glucose during binding to the enzyme
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the enzyme initiates the intracellular glucose catabolism/metabolism, overview
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the enzyme initiates the intracellular glucose catabolism/metabolism, overview
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway
-
-
ir
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
-
-
-
-
?
additional information

?
-
hexokinase, Hxk1, but not the glucokinase, Glk1, is required for normal growth and sugar metabolism, and for pathogenicity on fruits
-
-
?
additional information
?
-
-
hexokinase, Hxk1, but not the glucokinase, Glk1, is required for normal growth and sugar metabolism, and for pathogenicity on fruits
-
-
?
additional information
?
-
-
loss of enzyme activity is involved in type 2 diabetes mellitus
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-deoxy-2-([[(1,1-dioxido-1-benzothiophen-2-yl)methoxy]carbonyl]amino)-beta-D-glucopyranose
-
2-deoxy-2-[[3-(4-hydroxyphenyl)propanoyl]amino]-alpha-D-glucopyranose
186fold selectivity for Trypanosoma cruzi over human enzyme
2-[[(benzyloxy)carbonyl]amino]-2-deoxy-beta-D-glucopyranose
245fold selectivity for Trypanosoma cruzi over human enzyme
6-Amino-6-deoxy-D-glucose
-
noncompetitive to ATP and competitive to glucose
adenosine 5'-diphosphate-polyamidoamine dendrimer
-
product inhibition
-
beta,gamma-methyleneadenosine 5'-thiophosphate
-
noncompetitive to ATP and uncompetitive to glucose
D-fructose 6-phosphate
-
weak
GK regulatory protein
-
-
-
glucokinase regulatory protein
-
glucokinase-regulatory protein
GK regulatory protein, relative inhibition of glucokinase activity through GKRP alone wild-type: 32.5% and GKRP plus 10 microM sorbitol 6-phosphate: 55
-
human glucokinase regulatory protein
-
inhibition is reversed by activator RO-28-1675
-
N-acetyl-alpha-D-glucosamine
-
-
p-chlormercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
palmitoyl-CoA
-
glucokinase is inhibited by endogenous long-chain fatty acyl-CoA in islets from omega3-depleted rats
ADP

-
-
ADP
-
noncompetitive to ATP and uncompetitive to glucose
CTP

-
in presence of ATP
CTP
-
above 1 mM, irrespective of glucose concentration
D-glucose 6-phosphate

-
competitive inhibitor to glucose and noncompetitive to ATP
D-glucose 6-phosphate
-
-
D-glucose 6-phosphate
-
-
D-glucose 6-phosphate
-
-
D-glucose 6-phosphate
-
weak
D-glucose 6-phosphate
-
-
glucokinase regulatory protein

-
human, i.e. GKRP, recombinantly expressed in Escherichia coli, competitive to glucose, strong inhibition in absence of fructose 6-phosphate or sorbitol 6-phosphate, the protein also binds fructose 1-phosphate and chloride are reversing the inhibition
-
glucokinase regulatory protein
-
of human or rat origin, inhibits the wild-type enzyme in presence or absence of sorbitol 6-phosphate, no inhibition of mutant V62M by both proteins
-
glucokinase regulatory protein
-
i.e. GKRP, recombinantly expressed in Escherichia coli, inhibition in absence of fructose 6-phosphate or sorbitol 6-phosphate, competitive to D-glucose, fructose 1-phosphate and chloride are reversing the inhibition
-
GTP

-
in presence of ATP
GTP
-
above 1 mM, irrespective of glucose concentration
ITP

-
in presence of ATP
ITP
-
above 1 mM, irrespective of glucose concentration
N-acetylglucosamine

-
-
N-acetylglucosamine
-
weak
TTP

-
in presence of ATP
TTP
-
above 1 mM, irrespective of glucose concentration
UTP

-
in presence of ATP
UTP
-
above 1 mM, irrespective of glucose concentration
additional information

-
not: glucose or ATP up to 15 mM, mannose, fructose, 2-deoxyglucose, GDP, IDP, CDP, UDP, adenosine monophosphate, deoxyadenosine monophosphate, 3',5'-adenosine monophosphate, inorganic phosphate
-
additional information
-
catabolite repression of the glucokinase pathway and the TOL pathway is triggered by two different catabolite repression systems, repression of the glucokinase pathway is channeled through Crc
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2R)-3-cyclopentyl-2-[4-(methylsulfonyl)phenyl]-N-(1,3-thiazol-2-yl)propanamide
-
activator associates with glucokinase in a glucose-independent fashion. Kinetic assays reveal a lag in enzyme progress curves that is systematically reduced when the enzyme is preincubated with the activator. Activator binding is enthalpically driven. The kcat value of glucokinase is almost fully limited by product release, both in the presence and absence of activator
(S)-3-(4-((3-fluoropyrrolidin-1-yl)sulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)-N-(thiazol-2-yl)benzamide
-
activation: 1.9fold
(S)-3-(4-((3-fluoropyrrolidin-1-yl)sulfonyl)phenoxy)-N-(5-fluorothiazol-2-yl)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 2.2fold
(S)-6-(3-cyclopentyl-2-[4-(trifluoromethyl)-1H-imidazol-1-yl]propanamido)nicotinic acid
-
in the presence of liver-specific GKA, progress curves at 1 mM glucose are similar to those at 5 mM, reflecting activation of GK. With steady-state kinetic methods it is shown that there are at least two kinetically distinct forms of glucokinase that interconvert through a slow conformational change and that this interconversion is affected by glucose concentration and a liver-specific GKA
(Z)-2-(4-(cyclopropylsulfonyl)phenyl)-N-(5-(2-methylpropylidene)-4-oxo-4,5-dihydro thiazol-2-yl)-3-(tetrahydro-2Hpyran-4-yl)propanamide
-
activation: 1.17fold
(Z)-N-(5-benzylidene-4-oxo-4,5-dihydrothiazol-2-yl)-2-(4-(cyclopropylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.24fold
1-[6'-(2-hydroxy-2-methylpropoxy)-4-[(5-methylpyridin-3-yl)oxy]-3,3'-bipyridin-6-yl]-3-methylurea
i.e. AM-2394. Compound activates glucokinase with an EC50 of 60 nM, increases the affinity of glucokinase for glucose by approximately 10fold, exhibits moderate clearance and good oral bioavailability in multiple animal models, and lowers glucose excursion following an oral glucose tolerance test in an ob/ob mouse model of diabetes
2-(4-(cyclopropylsulfonyl)phenyl)-3-(tetrahydro-2Hpyran-4-yl)-N-(4,5,6,7-tetrahydrobenzo[d]thiazol-2-yl)propanamide
-
activation: 2.27fold
2-(4-(cyclopropylsulfonyl)phenyl)-N-(4-(prop-1-en-2-yl)thiazol-2-yl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 2.4fold
2-(4-(cyclopropylsulfonyl)phenyl)-N-(4-isopropylthiazol-2-yl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 2.97fold
2-(4-(cyclopropylsulfonyl)phenyl)-N-(4-oxo-4,5-dihydrothiazol-2-yl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.65fold
2-(4-(cyclopropylsulfonyl)phenyl)-N-(5,6-dihydro-4Hcyclopenta[d]thiazol-2-yl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 3.4fold
2-(4-(cyclopropylsulfonyl)phenyl)-N-(5-iodo-4-isopropylthiazol-2-yl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.34fold
2-(4-(cyclopropylsulfonyl)phenyl)-N-(5-methyl-4,5,6,7-tetrahydrothiazolo[5,4-c] pyridin-2-yl)-3-(tetrahydro-2Hpyran-4-yl)propanamide
-
activation: 2.26fold
2-(4-(methylsulfonyl)phenyl)-N-(4-phenylthiazol-2-yl)-3-(tetrahydro-2H-pyran-4-yl)-propanamide
-
activation: 1.3fold
2-amino-4-fluoro-5-(1-methyl-1H-imidazol-2-ylsulfanyl)-N-thiazol-2-yl-benzamide
potent synthetic allosteric activator, acts on the wild-type and the N-terminal deletion mutants DELTA N1-11 and DELTA N1-15, mechanism of activation
2-amino-N-(4-methyl-1,3-thiazol-2-yl)-5-[(4-methyl-4H-1,2,4-triazol-3-yl)sulfanyl]benzamide
-
activator associates with glucokinase in a glucose-independent fashion. Kinetic assays reveal a lag in enzyme progress curves that is systematically reduced when the enzyme is preincubated with the activator. Activator binding is enthalpically driven. The kcat value of glucokinase is almost fully limited by product release, both in the presence and absence of activator
2-[4-(methylsulfonyl)phenyl]-3-(tetrahydro-2H-pyran-4-yl)-N-(1,3-thiazol-2-yl)propanamide
-
activation: 2.45fold
3-((3-methylbut-2-en-1-yl)oxy)-5-(4-(morpholinosulfonyl)phenoxy)-N-(thiazol-2-yl)benzamide
-
activation: 2.1fold
3-(4-(((2S,6R)-2,6-dimethylmorpholino)sulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)-N-(thiazol-2-yl)benzamide
-
activation: 1.5fold
3-(4-(((2S,6R)-2,6-dimethylmorpholino)sulfonyl)phenoxy)-N-(5-fluorothiazol-2-yl)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 1.7fold
3-(4-((4-methoxypiperidin-1-yl)sulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)-N-(thiazol-2-yl)benzamide
-
activation: 1.7fold
3-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-ylsulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)-N-(thiazol-2-yl)benzamide
-
activation: 1.7fold
3-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-ylsulfonyl)phenoxy)-N-(5-fluorothiazol-2-yl)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 1.9fold
3-(4-(cyclopropylsulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)-N-(4-oxo-4,5-dihydrothiazol-2-yl)benzamide
-
activation: 1.5fold
3-(4-(cyclopropylsulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)-N-(5-methylpyrazin-2-yl)benzamide
-
activation: 1.6fold
3-(4-(cyclopropylsulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)-N-(thiazol-2-yl)benzamide
-
activation: 2.4fold
3-(4-(cyclopropylsulfonyl)phenoxy)-N-(1,5-dimethyl-1H-pyr-azol-3-yl)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 1.5fold
3-(4-(cyclopropylsulfonyl)phenoxy)-N-(4-(4-fluorophenyl)thiazol-2-yl)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 0.9fold
3-(4-(cyclopropylsulfonyl)phenoxy)-N-(5-fluorothiazol-2-yl)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 1.8fold
6-[[3-hydroxy-5-(propan-2-yloxy)benzoyl]amino]pyridine-3-carboxylic acid
-
activator associates with glucokinase in a glucose-independent fashion. Kinetic assays reveal a lag in enzyme progress curves that is systematically reduced when the enzyme is preincubated with the activator. Activator binding is enthalpically driven. The kcat value of glucokinase is almost fully limited by product release, both in the presence and absence of activator
N-(4,5-dimethylthiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.72fold
N-(4-(4-fluorophenyl)thiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.25fold
N-(4-(4-methoxyphenyl)thiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.22fold
N-(4-(tert-butyl)thiazol-2-yl)-3-(4-(cyclopropylsulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 0.7fold
N-(4-cyclopropylthiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.87fold
N-(4-ethylthiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)-propanamide
-
activation: 2.17fold
N-(4-isobutylthiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)-propanamide
-
activation: 2.05fold
N-(4-isopropylthiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)-propanamide
-
activation: 2.49fold
N-(4-tert-butylthiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)-propanamide
-
activation: 1.29fold
N-(5-bromo-4-isopropylthiazol-2-yl)-2-(4-(cyclopropylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.67fold
N-(5-fluorothiazol-2-yl)-3-((3-methylbut-2-en-1-yl)oxy)-5-(4-(morpholinosulfonyl)phenoxy)benzamide
-
activation: 1.8fold
N-(5-fluorothiazol-2-yl)-3-(4-((4-methoxypiperidin-1-yl)sulfonyl)phenoxy)-5-((3-methylbut-2-en-1-yl)oxy)benzamide
-
activation: 1.7fold
N-(5-isopropyl-4-methylthiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.13fold
N-(6-fluorobenzo[d]thiazol-2-yl)-2-(4-(methylsulfonyl)phenyl)-3-(tetrahydro-2H-pyran-4-yl)propanamide
-
activation: 1.3fold
RO-0274375
-
synthetic activator, activation of wild-type enzyme and mutants V62A, V62T, and V62L, no activation of mutants V62Q, V62E, V62F, and V62K
-
RO-028165
-
synthetic activator, activation of wild-type enzyme and mutants V62A, V62T, and V62L, no activation of mutants V62Q, V62E, V62F, and V62K
-
RO-0283946
-
synthetic activator, activation of wild-type enzyme and mutants V62A, V62T, and V62L, no activation of mutants V62Q, V62E, V62F, and V62K
-
RO-28-0450
-
racemic, activates the enzyme
RO-28-1675

-
R-enantiomer, highly activates the enzyme by elevating Vmax 1.5fold and decreasing Km about 4fold, reverses enzyme inhibition by human glucokinase regulatory protein, the S-enantiomer is inactive
RO-28-1675
-
reduced blood glucose level in vivo after feeding to type 2 diabetic mice
RO-28-1675
-
lowers the threshold concentration of D-glucose required for insulin release from 7 mM to 3 mM in pancreatic islets in vivo, reduced blood glucose level in vivo after feeding to type 2 diabetic Goto-Kakizaki rats and supresses endogenous glucose production in ZDF-Gmi rats
additional information

-
RO-28-1674 is inactive
-
additional information
-
RO-28-1674 is inactive
-
additional information
-
the enzyme is 2.12fold induced by growth on glucose compared to citrate
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.9
2-deoxy-D-glucose
-
30°C, pH 7.5, S151A
18
2-deoxyglucose
-
30°C, pH 7.5, wild-type
0.5 - 0.67
6-N-(carboxyethyl)ATP
0.55 - 0.65
6-N-(carboxymethyl)ATP
0.38 - 1
6-N-(succinyl)ATP
0.5 - 1.25
6-N-[N-(6-aminohexhyl)carbamoyl]ATP
0.15
alpha-D-glucose
-
30°C, pH 7.6
additional information
additional information
-
0.5
6-N-(carboxyethyl)ATP

-
-
0.67
6-N-(carboxyethyl)ATP
-
-
0.55
6-N-(carboxymethyl)ATP

-
-
0.65
6-N-(carboxymethyl)ATP
-
-
0.38
6-N-(succinyl)ATP

-
-
0.5
6-N-[N-(6-aminohexhyl)carbamoyl]ATP

-
-
1.25
6-N-[N-(6-aminohexhyl)carbamoyl]ATP
-
-
0.00000023
ATP

-
wild-type in the presence of 20-30% glycerol, pH and temperature not specified in the publication
0.0000003
ATP
-
mutant W99R/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
0.00000035
ATP
-
wild-type in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000046
ATP
-
wild-type, pH and temperature not specified in the publication
0.00000049
ATP
-
mutant W99R/W257F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000055
ATP
-
mutant W99R/W257F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000057
ATP
-
wild-type in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000071
ATP
-
mutant W167F/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
0.00000071
ATP
-
mutant W99R/W167F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
0.00000078
ATP
-
mutant W99R/W257F, pH and temperature not specified in the publication
0.00000109
ATP
-
mutant W167F/W257F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000143
ATP
-
mutant W167F/W257F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000143
ATP
-
mutant W99R/W167F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000163
ATP
-
mutant W167F/W257F, pH and temperature not specified in the publication
0.00000294
ATP
-
mutant W99R/W167F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.00000335
ATP
-
mutant W99R/W167F, pH and temperature not specified in the publication
0.04
ATP
-
wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 0.5 mM D-glucose
0.07
ATP
-
recombinant mutant V62E
0.11
ATP
-
recombinant mutant V62Q
0.16
ATP
-
recombinant mutant V62K
0.16
ATP
mutant enzyme V182L, glutathione S-transferase glucokinase B fusion protein
0.16
ATP
-
wild-type, 37°C, pH not specified in the puclication
0.16
ATP
-
wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 60 mM D-glucose
0.189
ATP
-
pH 8.0, 85°C, recombinant enzyme
0.19
ATP
-
ATP in form of MgATP2-
0.2
ATP
-
recombinant mutant V62A
0.2
ATP
deletion mutant lacking residues 66-71, pH 7.6, 25 °C
0.2
ATP
deletion mutant lacking residues 68-71, pH 7.6, 25 °C
0.21
ATP
mutant enzyme Y61S, glutathione S-transferase glucokinase B fusion protein
0.22
ATP
-
recombinant mutant V62T
0.24
ATP
-
mutant L146R, 37°C, pH not specified in the puclication
0.26
ATP
-
recombinant Glk, pH 7.6, 25°C
0.27
ATP
-
30°C, pH 7.5, N166R
0.29
ATP
deletion mutant lacking residue 71, pH 7.6, 25 °C
0.3
ATP
deletion mutant lacking residues 67-71, pH 7.6, 25 °C
0.31
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant 454-Ala
0.31
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68V
0.32
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64P
0.35
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A456V
0.35
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K140E
0.36
ATP
-
recombinant enyme, pH 7.3, 80°C
0.38
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V455M
0.39
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68K
0.4
ATP
mutant enzyme E265K, glutathione S-transferase glucokinase B fusion protein
0.4
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197E
0.41
ATP
-
recombinant wild-type enzyme
0.41
ATP
mutant enzyme K420E, glutathione S-transferase glucokinase B fusion protein
0.45
ATP
wild-type glutathione S-transferase glucokinase B fusion protein
0.46
ATP
-
recombinant mutant V62M
0.46
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, wild-type
0.48
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V91L
0.5
ATP
mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C
0.5
ATP
wild-type, pH 7.6, 25 °C
0.51
ATP
-
recombinant YajF, pH 7.6, 25°C
0.52
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V62M
0.53
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V452L
0.57
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant T65I
0.58
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y215A
0.61
ATP
-
recombinant mutant V62L
0.63
ATP
-
30°C, pH 7.5, wild-type
0.63
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S263P
0.69
ATP
mutant enzyme A379V, glutathione S-transferase glucokinase B fusion protein
0.71
ATP
-
recombinant mutant V62F
0.72
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G72R
0.75
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C252Y
0.76
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V389L
0.87
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A379T
0.89
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C213R
0.92
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A
1.08
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214C
1.2
ATP
deletion mutant lacking residues 70-71, pH 7.6, 25 °C
1.31
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197L
1.39
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I
1.42
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A/V452A
1.5
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant E442K
1.52
ATP
-
30°C, pH 7.5, N166R-S151A
1.53
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K414E
1.59
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64Y
2.08
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant P417R
2.21
ATP
-
30°C, pH 7.5, S151A
2.26
ATP
-
30°C, pH 7.5, S151G
2.6
ATP
-
30°C, pH 7.5, S151C
2.71
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I/A379T
2.9
ATP
-
recombinant NanK, pH 7.6, 25°C
3.32
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M298K
3.4
ATP
-
recombinant YcfX, pH 7.6, 25°C
12.6
ATP
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S336L
4.7
D-fructose

-
30°C, pH 7.5, N166R-S151A
7.7
D-fructose
-
30°C, pH 7.5, S151G
57.3
D-fructose
-
30°C, pH 7.5, S151A
77
D-fructose
-
30°C, pH 7.5, N166R
240
D-fructose
-
30°C, pH 7.5, wild-type and S151C
0.028
D-glucose

-
0.038
D-glucose
-
pH 8.0, 85°C, recombinant enzyme
0.054
D-glucose
-
50°C, pH 8
0.076
D-glucose
-
recombinant Glk, pH 7.6, 25°C
0.095
D-glucose
-
30°C, pH 7
0.11
D-glucose
-
+ CTP, 30°C, pH 7
0.12
D-glucose
-
20°C pH 7
0.12
D-glucose
-
+ UTP, 30°C, pH 7
0.15
D-glucose
-
30°C, pH 7.5, S151G and N1166R-S151A
0.2
D-glucose
-
+ ITP, 30°C, pH 7
0.22
D-glucose
-
cosubstrate GTP, 30°C, pH 7
0.31
D-glucose
pH 9.0, 30°C
0.31
D-glucose
-
recombinant YajF, pH 7.6, 25°C
0.34
D-glucose
-
30°C, pH 7.5, S151A
0.5
D-glucose
-
+ ATP, 30°C, pH 7
0.61
D-glucose
-
22°C, pH 7.5
0.95
D-glucose
His-tagged recombinant protein, pH 7.2, 22°C
1
D-glucose
-
recombinant enyme, pH 7.3, 80°C
2
D-glucose
-
in presence of activator RO-28-1675
2 - 3
D-glucose
-
enzyme from active animals, S0.5 value, Hill coefficient 1.16, pH 7.8, 2°C
2.1
D-glucose
-
30°C, pH 7.5, S151C and N166R
2.4
D-glucose
pH 8.0, 75°C
2.5
D-glucose
deletion mutant lacking residues 68-71, K0.5 value, Hill coefficient 1.2, pH 7.6, 25 °C
2.5 - 5
D-glucose
recombinant enzyme without His6
3.1
D-glucose
deletion mutant lacking residue 71, K0.5 value, Hill coefficient 1.5, pH 7.6, 25 °C
3.2
D-glucose
deletion mutant lacking residues 67-71, K0.5 value, Hill coefficient 1.1, pH 7.6, 25 °C
3.3
D-glucose
recombinant (His)6-tagged enzyme
3.3
D-glucose
mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C
3.6
D-glucose
deletion mutant lacking residues 66-71, K0.5 value, Hill coefficient 1.2, pH 7.6, 25 °C
3.8
D-glucose
-
recombinant YcfX, pH 7.6, 25°C
6
D-glucose
-
30°C, pH 7.5, wild-type
6.23
D-glucose
-
enzyme from active animals, S0.5 value, Hill coefficient 1.7, pH 7.8, 25°C
6.3
D-glucose
wild-type, K0.5 value, Hill coefficient 1.8, pH 7.6, 25 °C
7.4
D-glucose
-
31°C and pH 7.7, fetal glucokinase
7.7
D-glucose
-
31°C and pH 7.7, adult glucokinase
8.6
D-glucose
-
in absence of activator
9
D-glucose
-
enzyme from hibernating animals, S0.5 value, Hill coefficient 1.6, pH 7.8, 25°C
10.6
D-glucose
deletion mutant lacking residues 70-71, K0.5 value, Hill coefficient 1.3, pH 7.6, 25 °C
18
D-glucose
-
recombinant NanK, pH 7.6, 25°C
45
D-glucose
-
enzyme from hibernating animals, S0.5 value, Hill coefficient 1.2, pH 7.8, 2°C
0.08
D-mannose

-
30°C, pH 7.5, N166R-S151A
0.12
D-mannose
-
30°C, pH 7.5, S151G
0.21
D-mannose
-
30°C, pH 7.5, S151A
2.2
D-mannose
-
30°C, pH 7.5, N166R
3.74
D-mannose
-
30°C, pH 7.5, S151C
4.4
D-mannose
-
30°C, pH 7.5, wild-type
additional information
additional information

-
kinetics
-
additional information
additional information
kinetic mechanism
-
additional information
additional information
-
kinetic mechanism
-
additional information
additional information
-
activation energy, kinetics
-
additional information
additional information
-
kinetic analysis, wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetics with cofactor ATP and with adenosine 5'-triphosphate-polyamidoamine dendrimer as cofactor, overview
-
additional information
additional information
positive cooperation, mechanism, kinetic model
-
additional information
additional information
-
positive cooperation, mechanism, kinetic model
-
additional information
additional information
-
wild-type and mutant enzymes, cooperative/sigmoidal glucose-dependent kinetics
-
additional information
additional information
kinetic constants of TcGlcK obtained for different anomer solutions of D-glucose
-
additional information
additional information
-
kinetic constants of TcGlcK obtained for different anomer solutions of D-glucose
-
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0.61
ATP

-
mutant L146R, 37°C, pH not specified in the puclication
0.7
ATP
-
wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 0.5 mM D-glucose
68.4
ATP
-
wild-type, 37°C, pH not specified in the puclication
68.4
ATP
-
wild-type, 37°C, pH not specified in the puclication. Kinetic constants at high and low concentrations of the fixed substrate: 60 mM D-glucose
5.9
beta-D-glucose

-
co-substrate: ATP, mutant W99R/W167F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
6.1
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
6.37
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W167F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
8.47
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F, pH and temperature not specified in the publication
9.43
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
11.1
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W167F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
12.4
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W167F, pH and temperature not specified in the publication
12.7
beta-D-glucose
-
co-substrate: ATP, mutant W167F/W257F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
26.2
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F in the presence of 20-30% glycerol, pH and temperature not specified in the publication
37.7
beta-D-glucose
-
co-substrate: ATP, wild-type in the presence of 20-30% glycerol, pH and temperature not specified in the publication
38.9
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
60.5
beta-D-glucose
-
co-substrate: ATP, wild-type in the presence of 20-30% glycerol and in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
69.9
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F, pH and temperature not specified in the publication
83.7
beta-D-glucose
-
co-substrate: ATP, wild-type, pH and temperature not specified in the publication
91.3
beta-D-glucose
-
co-substrate: ATP, mutant W99R/W257F in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
166
beta-D-glucose
-
co-substrate: ATP, wild-type in the presence of 20 microM glucokinase activator drug (GKA), pH and temperature not specified in the publication
0.007
D-glucose

-
mutant T228M, 37°C, pH not specified in the puclication
0.026
D-glucose
deletion mutant lacking residues 68-71, pH 7.6, 25 °C
0.037
D-glucose
deletion mutant lacking residues 66-71, pH 7.6, 25 °C
0.042
D-glucose
deletion mutant lacking residues 67-71, pH 7.6, 25 °C
0.77
D-glucose
-
mutant L146R, 37°C, pH not specified in the puclication
2.46
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S336L
4.92
D-glucose
-
recombinant mutant V62E
6.42
D-glucose
-
recombinant mutant V62K
7.8
D-glucose
His-tagged recombinant protein, pH 7.2, 22°C
9.1
D-glucose
-
recombinant NanK, pH 7.6, 25°C
9.2
D-glucose
-
recombinant YcfX, pH 7.6, 25°C
12
D-glucose
-
recombinant Glk, pH 7.6, 25°C
14.9
D-glucose
-
recombinant mutant V62Q
17.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197E
19.9
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K414E
22.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant T65I
23.1
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A/V452A
25.7
D-glucose
-
recombinant mutant V62T
29
D-glucose
deletion mutant lacking residues 70-71, pH 7.6, 25 °C
29.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C252Y
29.8
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G72R
33
D-glucose
deletion mutant lacking residue 71, pH 7.6, 25 °C
33.7
D-glucose
-
recombinant mutant V62F
37.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M298K
40
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant K140E
40.1
D-glucose
wild-type, pH 7.6, 25 °C
43.1
D-glucose
-
recombinant mutant V62A
43.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68K
44.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S263P
44.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y215A
47.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V62M
48.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant P417R
50
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant C213R
50.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I/A379T
52.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant 454-Ala
52.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant E442K
53.6
D-glucose
-
recombinant mutant V62M
56
D-glucose
mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C
58.1
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197I
59.9
D-glucose
-
recombinant mutant V62L
60.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V91L
61.2
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A379T
61.8
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V455M
62.3
D-glucose
-
recombinant wild-type enzyme
62.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant M197L
62.7
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant G68V
62.8
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, wild-type
65.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214C
65.6
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant A456V
67.1
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V389L
67.6
D-glucose
-
wild-type, 37°C, pH not specified in the puclication
83.3
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64P
92.6
D-glucose
-
pH 8.0, 85°C, recombinant enzyme
114
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant S64Y
117
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant Y214A
122
D-glucose
pH 7.1, temperature not specified in the publication, co-substrate: ATP, mutant V452L
410
D-glucose
-
recombinant Glk, pH 7.6, 25°C
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