Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.194 extracted from

  • Luo, P.; Dai, S.; Zeng, J.; Duan, J.; Shi, H.; Wang, J.
    Inward-facing conformation of L-ascorbate transporter suggests an elevator mechanism (2018), Cell Discov., 4, 35 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.194

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43 (DE3) cells Pasteurella multocida

Crystallization (Commentary)

Crystallization (Comment) Organism
EIICasc in the inward-facing conformation, hanging drop vapor diffusion method, using 0.28 M CaCl2, 0.1M Tris-HCl, pH 7.5,42% (w/v) PEG400 Pasteurella multocida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-Npi-phospho-L-histidine + L-ascorbate[side 1] Pasteurella multocida
-
 [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?
[protein]-Npi-phospho-L-histidine + L-ascorbate[side 1] Pasteurella multocida Pm70
-
 [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Pasteurella multocida Q9CMQ1
-
-
Pasteurella multocida Pm70 Q9CMQ1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pasteurella multocida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[protein]-Npi-phospho-L-histidine + L-ascorbate[side 1]
-
 Pasteurella multocida [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?
[protein]-Npi-phospho-L-histidine + L-ascorbate[side 1]
-
 Pasteurella multocida Pm70 [protein]-L-histidine + L-ascorbate 6-phosphate[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
EIICasc
-
 Pasteurella multocida
L-ascorbate transporter
-
 Pasteurella multocida
UlaA
-
 Pasteurella multocida

General Information

General Information Comment Organism
metabolism the phosphotransferase system PTSasc is composed of two soluble energy-coupling proteins (EI and HPr) and an enzyme II complex (EIIA, EIIB, and EIIC) for the anaerobic uptake of ascorbate and its phosphorylation to L-ascorbate 6-phosphate in vivo Pasteurella multocida