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Literature summary for 2.7.1.174 extracted from

  • Qiu, Y.; Hassaninasab, A.; Han, G.S.; Carman, G.M.
    Phosphorylation of Dgk1 diacylglycerol kinase by casein kinase II regulates phosphatidic acid production in Saccharomyces cerevisiae (2016), J. Biol. Chem., 291, 26455-26467 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.174

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
S44A the mutation does not affect phosphorylation of the enzyme Saccharomyces cerevisiae
S44A/S45A/S46A the mutations abolish the stationary phase-dependent stimulation of enzyme activity. The phosphorylation-deficient mutations decrease enzyme function in phosphatidic acid production and in eliciting pah1DELTA phenotypes, such as the expansion of the nuclear/endoplasmic reticulum membrane, reduced lipid droplet formation, and temperature sensitivity Saccharomyces cerevisiae
S46A the mutation abolishes the stationary phase-dependent stimulation of enzyme activity. The phosphorylation-deficient mutation decreases enzyme function in phosphatidic acid production and in eliciting pah1DELTA phenotypes, such as the expansion of the nuclear/endoplasmic reticulum membrane, reduced lipid droplet formation, and temperature sensitivity Saccharomyces cerevisiae
S5A the mutation does decreases phosphorylation of the enzyme by about 40% compared to the wild type enzyme Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Saccharomyces cerevisiae 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 1,2-diacyl-sn-glycerol Saccharomyces cerevisiae
-
 CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
CTP + 1,2-diacyl-sn-glycerol Saccharomyces cerevisiae BY4741
-
 CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12382
-
-
Saccharomyces cerevisiae BY4741 Q12382
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme is phosphorylated by casein kinase II at serine residues Ser45 and Ser46. The casein kinase II-mediated phosphorylation of the enzyme regulates its function in the production of phosphatidic acid. The enzyme is inhibited by dephosphorylation with alkaline phosphatase Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 1,2-diacyl-sn-glycerol
-
 Saccharomyces cerevisiae CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
CTP + 1,2-diacyl-sn-glycerol
-
 Saccharomyces cerevisiae BY4741 CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
additional information the enzyme is a bona fide substrate of casein kinase II and its phosphorylation stimulates enzyme activity Saccharomyces cerevisiae ?
-
-
additional information the enzyme is a bona fide substrate of casein kinase II and its phosphorylation stimulates enzyme activity Saccharomyces cerevisiae BY4741 ?
-
-

Subunits

Subunits Comment Organism
? x * about 30000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
DAG kinase
-
 Saccharomyces cerevisiae
DGK1
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism the enzyme in conjunction with Pah1 phosphatidic acid phosphatase controls the levels of phosphatidic acid and diacylglycerol for the synthesis of triacylglycerol and membrane phospholipids, the growth of the nuclear/endoplasmic reticulum membrane, and the formation of lipid droplets Saccharomyces cerevisiae