Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | 37°C, pH 7.5 | Thermotoga maritima | |
0.22 | - |
D-xylulose | 37°C, pH 7.5 | Thermotoga maritima | |
0.7 | - |
ATP | 60°C, pH 7.5 | Thermotoga maritima | |
1.24 | - |
D-xylulose | 60°C, pH 7.5 | Thermotoga maritima | |
17 | - |
(Polyphosphate)n | 37°C, pH 7.5 | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WXX1 | - |
- |
Thermotoga maritima DSM 3109 | Q9WXX1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(polyphosphate)n + D-xylulose | activity with polyphosphate is 41 % of its specific activity on ATP | Thermotoga maritima | (polyphosphate)n-1 + D-xylulose 5-phosphate | - |
? | |
(polyphosphate)n + D-xylulose | activity with polyphosphate is 41 % of its specific activity on ATP | Thermotoga maritima DSM 3109 | (polyphosphate)n-1 + D-xylulose 5-phosphate | - |
? | |
ATP + D-xylulose | - |
Thermotoga maritima | ADP + D-xylulose 5-phosphate | - |
? | |
ATP + D-xylulose | - |
Thermotoga maritima DSM 3109 | ADP + D-xylulose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
ATP/polyphosphate xylulokinase | - |
Thermotoga maritima |
TM0116 | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 95 | 65°C: about 60% of maximal activity, 95°: about 60% of maximal activity | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
degradation constants at 0.1 and 0.01 g/l xylulokinase at 60°C are 0.20/h and 0.35/h, respectively. The presence of 2 mM xylulose enhances the thermostability of 0.01 g/l xylulokinase, increasing degradation constants to 0.29/h | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.77 | - |
(Polyphosphate)n | 37°C, pH 7.5 | Thermotoga maritima | |
24 | - |
ATP | 37°C, pH 7.5 | Thermotoga maritima | |
26 | - |
D-xylulose | 37°C, pH 7.5 | Thermotoga maritima | |
78 | - |
D-xylulose | 60°C, pH 7.5 | Thermotoga maritima | |
83 | - |
ATP | 60°C, pH 7.5 | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
62 | - |
D-xylulose | 60°C, pH 7.5 | Thermotoga maritima | |
116 | - |
D-xylulose | 37°C, pH 7.5 | Thermotoga maritima | |
119 | - |
ATP | 60°C, pH 7.5 | Thermotoga maritima | |
171 | - |
ATP | 37°C, pH 7.5 | Thermotoga maritima | |
516 | - |
(Polyphosphate)n | 37°C, pH 7.5 | Thermotoga maritima |