BRENDA - Enzyme Database show
show all sequences of 2.7.1.162

Efficient chemoenzymatic synthesis of uridine 5-diphosphate N-acetylglucosamine and uridine 5-diphosphate N-trifluoacetyl glucosamine with three recombinant enzymes

Li, X.; Qi, C.; Wei, P.; Huang, L.; Cai, J.; Xu, Z.; Prep. Biochem. Biotechnol. 47, 852-859 (2017)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the enzyme is used in the chemoenzymatic synthesis of UDP-GlcNAc and UDP-GlcNTFA attempted by three recombinant enzymes in a fed batch system, overview. The process can be used for the development of an efficient scalable process for the supply of UDP-monosaccharide donors for oligosaccharide synthesis
Escherichia coli
Cloned(Commentary)
Commentary
Organism
recombinant expression in Escherichia coli strain BL21(DE3)
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + N-acetyl-D-glucosamine
recombinant enzyme
739523
Escherichia coli
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-trifluoroacetyl-D-glucosamine
recombinant enzyme
739523
Escherichia coli
ADP + N-trifluoroacetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
the enzyme is used in the chemoenzymatic synthesis of UDP-GlcNAc and UDP-GlcNTFA attempted by three recombinant enzymes in a fed batch system, overview. The process can be used for the development of an efficient scalable process for the supply of UDP-monosaccharide donors for oligosaccharide synthesis
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression in Escherichia coli strain BL21(DE3)
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + N-acetyl-D-glucosamine
recombinant enzyme
739523
Escherichia coli
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-trifluoroacetyl-D-glucosamine
recombinant enzyme
739523
Escherichia coli
ADP + N-trifluoroacetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
Other publictions for EC 2.7.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739523
Li
Efficient chemoenzymatic synth ...
Escherichia coli
Prep. Biochem. Biotechnol.
47
852-859
2017
-
1
1
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-
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1
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-
-
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2
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1
1
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-
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737770
Sato
Open-close structural change u ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Biochim. Biophys. Acta
1854
333-340
2015
-
-
1
1
4
-
-
8
-
1
-
2
-
5
-
-
1
1
-
-
-
-
4
2
1
-
-
8
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
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8
-
1
-
2
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-
-
1
-
-
-
-
4
2
1
-
-
8
1
-
-
-
-
1
1
-
-
-
737343
Wang
Insights into the binding spec ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Acta Crystallogr. Sect. D
70
1401-1410
2014
-
-
2
2
17
-
-
1
-
2
2
3
-
12
-
-
2
2
-
-
-
-
24
3
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
2
17
-
-
-
-
1
-
2
2
3
-
-
-
2
-
-
-
-
24
3
2
-
-
-
2
-
-
-
-
4
4
-
-
-
726089
Li
Efficient enzymatic synthesis ...
Bifidobacterium longum subsp. infantis
Org. Lett.
15
5528-5530
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723238
Li
Substrate promiscuity of N-ace ...
Bifidobacterium longum, Bifidobacterium longum ATCC 55813, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Molecules
16
6396-6407
2011
-
-
2
-
-
-
-
8
-
4
-
-
-
11
-
-
-
-
-
-
-
-
36
-
-
-
-
8
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
8
-
4
-
-
-
-
-
-
-
-
-
-
36
-
-
-
-
8
2
-
-
-
-
-
-
-
8
8
702588
Cai
Substrate specificity of N-ace ...
Bifidobacterium longum
Bioorg. Med. Chem. Lett.
19
5433-5435
2009
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
11
-
1
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-
-
1
-
-
-
-
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-
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-
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-
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-
-
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-
1
-
1
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
703179
Cai
A chemoenzymatic route to N-ac ...
Bifidobacterium longum
Chem. Commun. (Camb. )
2009
2944-2946
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
-
-
-
-
-
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11
-
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-
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1
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-
-
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-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677700
Nishimoto
Identification of N-acetylhexo ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
73
6444-6449
2007
1
-
1
-
-
1
-
3
-
6
-
6
-
7
-
-
1
1
-
1
13
-
17
-
1
-
1
3
1
-
1
3
-
-
-
1
-
1
3
-
-
1
-
-
-
3
-
6
-
6
-
-
-
1
-
1
13
-
17
-
1
-
1
3
1
-
1
-
-
-
-
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-