Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Rosetta2 (DE3) and baculovirus-infected Sf9 insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-[(2E)-3-(2-furyl)prop-2-enylidene]-3-[(phenylsulfonyl)amino]2-thioxo-1,3-thiazolidin-4-one | i.e. CU-3, ATP competitive inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
ATP | isoform DGKalpha, at pH 7.4 and 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.001 mM used in assay conditions. The enzyme is positively regulated by Ca2+ | Homo sapiens | |
Mg2+ | 10 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | Homo sapiens | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23743 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, Superdex 200 gel filtration or Superdex 75 gel filtration | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, purified enzyme in 20 mM Tris-HCl, pH 7.4 with 200 mM NaCl, 3 mM CaCl2, 3 mM MgCl2, 0.5 mM dithiothreitol, and 5% (v/v) glycerol, at least 3 months, no loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | - |
Homo sapiens | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-dioleolyl-sn-glycerol | - |
Homo sapiens | ADP + 1,2-dioleolyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000, His6-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DGKalpha | isoform | Homo sapiens |
diacylglycerol kinase alpha | isoform | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00034 | - |
isoform DGKalpha, at pH 7.4 and 30°C | Homo sapiens | 5-[(2E)-3-(2-furyl)prop-2-enylidene]-3-[(phenylsulfonyl)amino]2-thioxo-1,3-thiazolidin-4-one |