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Literature summary for 2.7.1.107 extracted from

  • Vaz Dias, F.; Serrazina, S.; Vitorino, M.; Marchese, D.; Heilmann, I.; Godinho, M.; Rodrigues, M.; Malho, R.
    A role for diacylglycerol kinase 4 in signalling crosstalk during Arabidopsis pollen tube growth (2019), New Phytol., 222, 1434-1446 .
    View publication on PubMed
Search for more literature for 2.7.1.107

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM used in assay conditions Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q1PDI2 ecotype Col-0
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephadex G-25M gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
 Arabidopsis thaliana
-
pollen tube
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-dioleoyl-sn-glycerol
-
 Arabidopsis thaliana ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
additional information isoform DGK4 has also guanylyl cyclase activity Arabidopsis thaliana ?
-
-

Synonyms

Synonyms Comment Organism
DGK4 isoform Arabidopsis thaliana
diacylglycerol kinase 4 isoform Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction disruption of isoform DGK4 does not impair male gametophytic development but reduces in vivo performance and alters pollen tube Youngs modulus and adhesion properties Arabidopsis thaliana
physiological function enzyme-deficient pollen tubes exhibit altered membrane recycling and phospholipid levels Arabidopsis thaliana