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Literature summary for 2.7.1.107 extracted from

  • Delgado, R.; Munoz, Y.; Pena-Cortes, H.; Giavalisco, P.; Bacigalupo, J.
    Diacylglycerol activates the light-dependent channel TRP in the photosensitive microvilli of Drosophila melanogaster photoreceptors (2014), J. Neurosci., 34, 6679-6686.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene rdgA Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
additional information generation of a mutant defective in the gene encoding DGK, rdgA Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
R59022 a specific pharmacological enzyme inhibitor Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane rhabdomeral membrane, the enzyme is located predominantly in the cytoplasmic subrhabdomeral cisternae, a cytoplasmic membrane system located at close distance from the microvilli. Excised membrane patches retain a functional enzyme DGK Drosophila melanogaster 16020
-
rhabdomere rhabdomeral membrane, the enzyme is located predominantly in the cytoplasmic subrhabdomeral cisternae, a cytoplasmic membrane system located at close distance from the microvilli. Excised membrane patches retain a functional enzyme DGK Drosophila melanogaster 16028
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol Drosophila melanogaster
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol Drosophila melanogaster Oregon-R
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q09103 gene rdgA
-
Drosophila melanogaster Oregon-R Q09103 gene rdgA
-

Source Tissue

Source Tissue Comment Organism Textmining
eye
-
Drosophila melanogaster
-
photoreceptor
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol
-
Drosophila melanogaster ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol
-
Drosophila melanogaster Oregon-R ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
DAG-kinase
-
Drosophila melanogaster
DGK
-
Drosophila melanogaster
eye-specific diacylglycerol kinase UniProt Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Drosophila melanogaster

General Information

General Information Comment Organism
physiological function light-dependent channel TRP in the light-sensitive microvilli of the photoreceptor's rhabdomere is opened by diacylglycerol and silenced by ATP, suggesting diacylglycerol kinase involvement. The ATP effect is abolished by inhibiting enzyme diacylglycerol kinase DGK and in the rdgA mutant, lacking functional DGK. Diacylglycerol activates TRP even in the presence of a DAG-lipase inhibitor, inconsistent with a requirement of polyunsaturated fatty acids in opening TRP. Diacylglycerol is the endogenous TRP agonist. ATP lowers the channel activity, the ATP effect is completely abolished by inhibition of the enzyme Drosophila melanogaster