Cloned (Comment) | Organism |
---|---|
expression in SF21 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-dioleoylglycerol | uncompetitive inhibition of isoforms diacylglycerol kinase alpha and zeta, no inhibition of isoform epsilon. Binds to a site on the alpha and zeta isoforms that is exposed as a consequence of the substrate binding to the active site, the chiral specificity of the isoforms thus mimicks the substrate specificity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value in mol% for substrate 1,2-dioleoylglycerol is 0.8 for isoform alpha, 0.52 for isoform zeta, pH 7.2, 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | isoform diacylglycerol kinase alpha requires Ca2+ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-dioleoyl-sn-glycerol | - |
Homo sapiens | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | preferred substrate of isoform diacylglycerol kinase epsilon | Homo sapiens | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 2,3-dioleoyl-sn-glycerol | - |
Homo sapiens | ADP + 2,3-diacyl-sn-glycerol 1-phosphate | isoform diacylglycerol kinase alpha 8.5%, zeta, 12%, epsilon 6% of the activity with 1,2-dioleoyl-sn-glycerol, respectively | ? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki value in mol% for inhibitor 2,3-dioleoylglycerol is 1.7 for isoform alpha, 1.07 for isoform zeta, pH 7.2, 30°C | Homo sapiens |