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Literature summary for 2.7.1.107 extracted from

  • Gomez-Merino, F.C.; Brearley, C.A.; Ornatowska, M.; Abdel-Haliem, M.E.; Zanor, M.I.; Mueller-Roeber, B.
    AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression (2004), J. Biol. Chem., 279, 8230-8241.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the isozyme DGK2 is induced by exposure to low temperatures, e.g. 4°C Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
AtDGK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as NusA/His6-tagged isozyme DGK2 in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
LiCl more than 50% inhibition at above 200 mM Arabidopsis thaliana
additional information isozyme DGK2 activity is affected by salts and detergents, no inhibition by Ca2+ Arabidopsis thaliana
NaCl more than 50% inhibition at above 200 mM Arabidopsis thaliana
R59022 specific inhibitor Arabidopsis thaliana
sodium deoxycholate at 5 mM Arabidopsis thaliana
Triton X-100 strong inhibition at up to 2% Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
1,2-dioleoyl-sn-glycerol pH 7.5, 25°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79400
-
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE Arabidopsis thaliana
140000
-
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacylglycerol Arabidopsis thaliana
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
additional information Arabidopsis thaliana the isozyme DGK2 is involved in cold signal transduction ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FFN7 2 isozymes DGK1 and DGK2
-

Purification (Commentary)

Purification (Comment) Organism
CHAPS is the most effective detergent for isozyme DGK2 solubilization, NusA/His6-tagged fusion isozyme DGK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf especially cauline Arabidopsis thaliana
-
additional information isozyme DGK2 expression in various tissues, expression pattern Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacylglycerol
-
Arabidopsis thaliana ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacylglycerol
-
Arabidopsis thaliana ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
ATP + 1,2-dioleoyl-sn-glycerol
-
Arabidopsis thaliana ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
Arabidopsis thaliana ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?
additional information the isozyme DGK2 is involved in cold signal transduction Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE Arabidopsis thaliana
More structural organization of isozymes containing a 1,2-diacylglycerol/phorbolester-binding domain 1, a zinc-finger dependent C1 domain, and a 1,2-diacylglycerol accessory domain, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
DGK2
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.8 rapid decrease below pH 6.5 and above pH 7.8 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana