Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the isozyme DGK2 is induced by exposure to low temperatures, e.g. 4°C | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
AtDGK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as NusA/His6-tagged isozyme DGK2 in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LiCl | more than 50% inhibition at above 200 mM | Arabidopsis thaliana | |
additional information | isozyme DGK2 activity is affected by salts and detergents, no inhibition by Ca2+ | Arabidopsis thaliana | |
NaCl | more than 50% inhibition at above 200 mM | Arabidopsis thaliana | |
R59022 | specific inhibitor | Arabidopsis thaliana | |
sodium deoxycholate | at 5 mM | Arabidopsis thaliana | |
Triton X-100 | strong inhibition at up to 2% | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
1,2-dioleoyl-sn-glycerol | pH 7.5, 25°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79400 | - |
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE | Arabidopsis thaliana |
140000 | - |
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | Arabidopsis thaliana | - |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
additional information | Arabidopsis thaliana | the isozyme DGK2 is involved in cold signal transduction | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FFN7 | 2 isozymes DGK1 and DGK2 | - |
Purification (Comment) | Organism |
---|---|
CHAPS is the most effective detergent for isozyme DGK2 solubilization, NusA/His6-tagged fusion isozyme DGK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | especially cauline | Arabidopsis thaliana | - |
additional information | isozyme DGK2 expression in various tissues, expression pattern | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | - |
Arabidopsis thaliana | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-diacylglycerol | - |
Arabidopsis thaliana | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
ATP + 1,2-dioleoyl-sn-glycerol | - |
Arabidopsis thaliana | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | - |
Arabidopsis thaliana | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
additional information | the isozyme DGK2 is involved in cold signal transduction | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE | Arabidopsis thaliana |
More | structural organization of isozymes containing a 1,2-diacylglycerol/phorbolester-binding domain 1, a zinc-finger dependent C1 domain, and a 1,2-diacylglycerol accessory domain, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
DGK2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.8 | rapid decrease below pH 6.5 and above pH 7.8 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |