Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.107 extracted from

  • Yamada, K.; Sakane, F.; Matsushima, N.; Kanoh, H.
    EF-hand motifs of alpha, beta and gamma isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes (1997), Biochem. J., 321, 59-64.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cDNA subcloned into the EcoRI site of the simian virus 40-based expression vector pSRE Homo sapiens
cDNA subcloned into the EcoRI site of the simian virus 40-based expression vector pSRE Rattus norvegicus
cDNA subcloned into the EcoRI site of the simian virus 40-based expression vector pSRE Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzyme requires Ca2+, dissociation constant is 0.0099 mM, hydrophobic region of the enzyme is masked by addition of Ca2+ Sus scrofa
Ca2+ activity is independent of Ca2+, dissociation constant is 0.00089 mM, binding of Ca2+ results in exposure of hydrophobic amino acids Rattus norvegicus
Ca2+ enzyme can bind approximately 2 mol of Ca2+ per mol Homo sapiens
Ca2+ enzyme can bind approximately 2 mol of Ca2+ per mol Rattus norvegicus
Ca2+ enzyme can bind approximately 2 mol of Ca2+ per mol Sus scrofa
Ca2+ activity is independent of Ca2+, dissociation constant is 0.00044 mM, hydrophobic region of the enzyme is masked by addition of Ca2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
DGKgamma
-
Rattus norvegicus
-
DGKbeta
-
Sus scrofa
-
DGKalpha
-
Sus scrofa DGKalpha
-
DGKalpha
-