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Literature summary for 2.7.1.107 extracted from

  • Russ, E.; Kaiser, U.; Sandermann, H.
    Lipid-dependent membrane enzymes. Purification to homogeneity and further characterization of diacylglycerol kinase from Escherichia coli (1988), Eur. J. Biochem., 171, 335-342.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
diacylglycerol 3-phosphate the enzyme apoprotein is attributed to a novel feedback activation involving diacylglycerol 3-phosphate Escherichia coli
lauryl maltoside activates in presence of 11 mM Triton X-100 Escherichia coli
n-hexyl beta-D-glucoside activates in presence of 11 mM Triton X-100 Escherichia coli
octyl beta-glucoside activates in presence of 11 mM Triton X-100 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
x * 14000 Da, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.767
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoyl-sn-glycerol + GTP
-
Escherichia coli GDP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 14000 Da, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
the membrane preparation shows a threefold stimulation by heating for 5 min followed by a gradual loss of activity, half-life: about 1 h. Butane-1-ol-dissolved enzyme has a half-life of about 45 min Escherichia coli