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Literature summary for 2.7.1.107 extracted from

  • Kanoh, H.; Sakane, F.; Yamada, K.
    Diacylglycerol kinase isozymes from brain and lymphoid tissues (1992), Methods Enzymol., 209, 162-172.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylserine activates Sus scrofa
sphingosine potently activates the 80000 Da enzyme Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
R59022 inhibits the 80000 Da enzyme form but not the 150000 Da enzyme form Sus scrofa
sphingosine inhibits the 150000 Da enzyme Sus scrofa
Triton X-100 extremely inhibitory to the 80000 Da enzyme and the 150000 Da enzyme form Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sus scrofa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mol of Ca2+ per mol of enzyme. Ca2+ plus phosphatidylserine markedly activates by reducing the Km value for ATP Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol Sus scrofa the enzyme may regulate the intracellular concentration of diacylglycerol ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol Sus scrofa the 80000 Da and the 150000 Da enzyme form do not possess specificity towards diacylglycerol molecular species ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the 80000 Da enzyme form is phosphorylated by an unidentified protein kinase Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
a heat-labile 80000 Da enzyme and a heat-stable 150000 Da enzyme Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Sus scrofa
-
lymphoid tissue
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.7
-
1500000 Da enzyme Sus scrofa
15
-
80000 Da enzyme Sus scrofa

Storage Stability

Storage Stability Organism
the 150000 Da enzyme is very unstable but can be stored at -80°C in presence of bovine serum albumin Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol the enzyme may regulate the intracellular concentration of diacylglycerol Sus scrofa ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + 1,2-diacyl-sn-glycerol the 80000 Da and the 150000 Da enzyme form do not possess specificity towards diacylglycerol molecular species Sus scrofa ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
ATP + sn-1,2-dioleoylglycerol
-
Sus scrofa ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
?