Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylserine | activates | Sus scrofa | |
sphingosine | potently activates the 80000 Da enzyme | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
R59022 | inhibits the 80000 Da enzyme form but not the 150000 Da enzyme form | Sus scrofa | |
sphingosine | inhibits the 150000 Da enzyme | Sus scrofa | |
Triton X-100 | extremely inhibitory to the 80000 Da enzyme and the 150000 Da enzyme form | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mol of Ca2+ per mol of enzyme. Ca2+ plus phosphatidylserine markedly activates by reducing the Km value for ATP | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | Sus scrofa | the enzyme may regulate the intracellular concentration of diacylglycerol | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-diacyl-sn-glycerol | Sus scrofa | the 80000 Da and the 150000 Da enzyme form do not possess specificity towards diacylglycerol molecular species | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the 80000 Da enzyme form is phosphorylated by an unidentified protein kinase | Sus scrofa |
Purification (Comment) | Organism |
---|---|
a heat-labile 80000 Da enzyme and a heat-stable 150000 Da enzyme | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
lymphoid tissue | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.7 | - |
1500000 Da enzyme | Sus scrofa |
15 | - |
80000 Da enzyme | Sus scrofa |
Storage Stability | Organism |
---|---|
the 150000 Da enzyme is very unstable but can be stored at -80°C in presence of bovine serum albumin | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | the enzyme may regulate the intracellular concentration of diacylglycerol | Sus scrofa | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1,2-diacyl-sn-glycerol | the 80000 Da and the 150000 Da enzyme form do not possess specificity towards diacylglycerol molecular species | Sus scrofa | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + sn-1,2-dioleoylglycerol | - |
Sus scrofa | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? |