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Literature summary for 2.7.1.1 extracted from

  • Aleshin, A.E.; Malfois, M.; Liu, X.; Kim, C.S.; Fromm, H.J.; Honzatko, R.B.; Koch, M.H.J.; Svergun, D.I.
    Nonaggregating mutant of recombinant human hexokinase I exhibits wild-type kinetics and rod-like conformations in solution (1999), Biochemistry, 38, 8359-8366.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNA encoding brain hexokinase I is cloned, expression of wild-type and nondimerizing mutant hexokinase I in Escherichia coli ZSC13 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of hexokinase I dimers Homo sapiens

Protein Variants

Protein Variants Comment Organism
E280A hexokinase I mutation eliminates hydrogen bonds between monomers of crystallographic dimers, triple mutant E280A/R283A/G284Y: nonaggregating mutant of recombinant hexokinase I, engineered by directed mutation to block dimerization, exhibits wild-type activity Homo sapiens
G284Y hexokinase I mutation introduces steric hindrance, triple mutant E280A/R283A/G284Y: nonaggregating mutant of recombinant hexokinase I, engineered by directed mutation to block dimerization, exhibits wild-type activity Homo sapiens
R283A hexokinase I mutation eliminates hydrogen bonds between monomers of crystallographic dimers, triple mutant E280A/R283A/G284Y: nonaggregating mutant of recombinant hexokinase I, engineered by directed mutation to block dimerization, exhibits wild-type activity Homo sapiens

General Stability

General Stability Organism
phosphate stabilizes the wild-type monomer of hexokinase I relative to the dimer, D-glucose 6-phosphate stabilizes the dimer relative to the monomer Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,5-Anhydro-D-glucitol 6-phosphate wild-type and nonaggregating interface mutant hexokinase I, inhibition is relieved by phosphate Homo sapiens
D-glucose 6-phosphate potent product inhibition, allosterically relieved by phosphate, wild-type hexokinase I and nondimerizing mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.057 0.061 D-glucose wild-type and nonaggregating interface mutant hexokinase I, pH 7.8 Homo sapiens
0.66 1.2 ATP wild-type and nonaggregating interface mutant hexokinase I, pH 7.8 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Homo sapiens hexokinase I governs the rate-limiting step of glycolysis in brain D-glucose 6-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P19367 nonaggregating mutant of recombinant hexokinase I
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and nondimerizing mutant hexokinase I, expressed in Escherichia coli ZSC13 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain hexokinase I Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60 61 wild-type and nonaggregating interface mutant hexokinase I, pH 7.8 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ATP
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP hexokinase I governs the rate-limiting step of glycolysis in brain Homo sapiens D-glucose 6-phosphate + ADP
-
 ?
additional information structure model of hexokinase I Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer wild-type hexokinase I is a dimer in the presence of glucose 6-phosphate and down to protein concentrations as low as 1 mg/ml Homo sapiens
monomer wild-type hexokinase I is a monomer in the presence of glucose and phosphate at protein concentrations up to 10 mg/ml Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information 6000/min, wild-type and nonaggregating interface mutant hexokinase I Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
15
-
 1,5-Anhydro-D-glucitol 6-phosphate nonaggregating interface mutant hexokinase I, pH 7.8 Homo sapiens
23
-
 1,5-Anhydro-D-glucitol 6-phosphate wild-type hexokinase I, pH 7.8 Homo sapiens