Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | hxk1+ expression increases strongly during growth in fructose or glycerol | Schizosaccharomyces pombe | |
additional information | hxk2+ expression is highest during growth in glycerol | Schizosaccharomyces pombe |
Cloned (Comment) | Organism |
---|---|
genes hxk1+ and hxk2+, encoding hexokinases 1 and 2, are cloned, sequenced and expressed in Saccharomyces cerevisiae WF8-2C | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-D-glucose | - |
Schizosaccharomyces pombe | |
D-glucosamine | - |
Schizosaccharomyces pombe | |
additional information | not inhibited by trehalose 6-phosphate; not inhibited by trehalose 6-phosphate | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
D-mannose | - |
Schizosaccharomyces pombe | |
0.13 | - |
D-mannose | - |
Schizosaccharomyces pombe | |
0.16 | - |
D-glucose | - |
Schizosaccharomyces pombe | |
1.5 | - |
D-fructose | - |
Schizosaccharomyces pombe | |
8.4 | 8.5 | D-glucose | - |
Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Schizosaccharomyces pombe |
53000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | P50521 | hexokinase 2 | - |
Schizosaccharomyces pombe | Q09756 | hexokinase 1 | - |
Schizosaccharomyces pombe PB003 | Q09756 | hexokinase 1 | - |
Schizosaccharomyces pombe PB004 | P50521 | hexokinase 2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + ATP | higher activity than with glucose or mannose | Schizosaccharomyces pombe | ADP + D-fructose 6-phosphate | - |
? | |
D-fructose + ATP | higher activity than with glucose or mannose | Schizosaccharomyces pombe PB003 | ADP + D-fructose 6-phosphate | - |
? | |
D-fructose + ATP | higher activity than with glucose or mannose | Schizosaccharomyces pombe PB004 | ADP + D-fructose 6-phosphate | - |
? | |
D-glucose + ATP | 11% of the activity with fructose | Schizosaccharomyces pombe | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | 59% of the activity with fructose | Schizosaccharomyces pombe | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | 11% of the activity with fructose | Schizosaccharomyces pombe PB003 | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | 59% of the activity with fructose | Schizosaccharomyces pombe PB004 | ADP + D-glucose 6-phosphate | - |
? | |
D-mannose + ATP | 42% of the activity with fructose | Schizosaccharomyces pombe | ADP + D-mannose 6-phosphate | - |
? | |
D-mannose + ATP | 23% of the activity with fructose | Schizosaccharomyces pombe | ADP + D-mannose 6-phosphate | - |
? | |
D-mannose + ATP | 23% of the activity with fructose | Schizosaccharomyces pombe PB003 | ADP + D-mannose 6-phosphate | - |
? | |
D-mannose + ATP | 42% of the activity with fructose | Schizosaccharomyces pombe PB004 | ADP + D-mannose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, calculated from the amino acid sequence | Schizosaccharomyces pombe |
monomer | 1 * 53000, calculated from the amino acid sequence | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Schizosaccharomyces pombe |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
2-deoxy-D-glucose | fructose as substrate | Schizosaccharomyces pombe | |
2.1 | - |
D-glucosamine | fructose as substrate | Schizosaccharomyces pombe | |
4 | - |
2-deoxy-D-glucose | fructose as substrate | Schizosaccharomyces pombe | |
11 | - |
D-glucosamine | fructose as substrate | Schizosaccharomyces pombe |