Literature summary for 2.6.1.97 extracted from

Mei, X.; Alvarez, J.; Bon Ramos, A.; Samanta, U.; Iwata-Reuyl, D.; Swairjo, M.
Crystal structure of the archaeosine synthase QueF-like -Insights into amidino transfer and tRNA recognition by the tunnel fold (2017), Proteins Struct. Funct. Bioinform., 85, 103-116 .

Search for more literature for 2.6.1.97

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pyrobaculum calidifontis

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme and enzyme in complex with preQ0, hanging drop vapor diffusion method, using 17-19% (w/v) PEG3350, 0.15 M potassium thiocyanate, and 0.05% (w/v) sodium azide	Pyrobaculum calidifontis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O	Pyrobaculum calidifontis	-	L-glutamate + archaeine15 in tRNA	-	?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O	Pyrobaculum calidifontis VA1	-	L-glutamate + archaeine15 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum calidifontis	A3MSP1	-	-
Pyrobaculum calidifontis VA1	A3MSP1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA resin column chromatography, and Superdex 200 gel filtration	Pyrobaculum calidifontis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O	-	Pyrobaculum calidifontis	L-glutamate + archaeine15 in tRNA	-	?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O	-	Pyrobaculum calidifontis VA1	L-glutamate + archaeine15 in tRNA	-	?

Subunits

Subunits	Comment	Organism
homodecamer	-	Pyrobaculum calidifontis

Synonyms

Synonyms	Comment	Organism
QueF-L	-	Pyrobaculum calidifontis
QueF-like	-	Pyrobaculum calidifontis

General Information

General Information	Comment	Organism
metabolism	the enzyme is responsible for the final step in the biosynthesis of archaeosine in the D-loop of tRNA	Pyrobaculum calidifontis

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Release 2023.1 (January 2023)