Cloned (Comment) | Organism |
---|---|
expressed as a glutathione S-transferase fusion protein in soluble form Escherichia coli DH5alpha cells | Haemaphysalis longicornis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000217 | - |
2-oxobutyrate | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
0.00438 | - |
2-oxo-4-methylthiobutyrate | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
0.0175 | - |
pyruvate | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
0.1116 | - |
L-kynurenine | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
0.2062 | - |
3-hydroxykynurenine | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43100 | - |
gel filtration | Haemaphysalis longicornis |
45000 | - |
SDS-PAGE | Haemaphysalis longicornis |
46480 | - |
calculated from amino acid sequence | Haemaphysalis longicornis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Haemaphysalis longicornis | the purified recombinant KAT shows dose-dependent inhibition effect on the growth of equine babesial parasite, Babesia caballi, in in vitro culture | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemaphysalis longicornis | B1B6U5 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Haemaphysalis longicornis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Haemaphysalis longicornis | - |
midgut | - |
Haemaphysalis longicornis | - |
ovary | - |
Haemaphysalis longicornis | - |
salivary gland | - |
Haemaphysalis longicornis | - |
synganglion | - |
Haemaphysalis longicornis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxykynurenine + pyruvate | - |
Haemaphysalis longicornis | ? | - |
? | |
L-kynurenine + 2-oxo-4-methylthiobutyrate | - |
Haemaphysalis longicornis | ? | - |
? | |
L-kynurenine + 2-oxobutyrate | - |
Haemaphysalis longicornis | ? | - |
? | |
L-kynurenine + pyruvate | - |
Haemaphysalis longicornis | kynurenic acid + L-alanine + H2O | - |
? | |
additional information | the purified recombinant KAT shows dose-dependent inhibition effect on the growth of equine babesial parasite, Babesia caballi, in in vitro culture | Haemaphysalis longicornis | ? | - |
? | |
additional information | no activity towards L-tryptophan and pyruvic acid | Haemaphysalis longicornis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KAT | - |
Haemaphysalis longicornis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Haemaphysalis longicornis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | 55 | - |
Haemaphysalis longicornis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
the enzymatic activities starts to decrease dramatically when the temperature is above 55°C | Haemaphysalis longicornis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
2-oxobutyrate | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
0.16 | - |
2-oxo-4-methylthiobutyrate | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
2.02 | - |
pyruvate | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
55.5 | - |
L-kynurenine | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis | |
65.12 | - |
3-hydroxykynurenine | in 200 mM phosphate buffer, pH 7.5, at 37°C | Haemaphysalis longicornis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Haemaphysalis longicornis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Haemaphysalis longicornis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a highly conserved pyridoxal 5'-phosphate binding site (Gly-Ser-Ala-Gly-Lys-Thr-Phe-Ser) is located between Gly-219 and Ser-226 | Haemaphysalis longicornis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Haemaphysalis longicornis | calculated from amino acid sequence | - |
7.4 |
Organism | Comment | Expression |
---|---|---|
Haemaphysalis longicornis | protein expression levels increase during the blood-feeding course | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.2 | - |
2-oxobutyrate | in 200 mM phosphate buffer, pH7.5, at 37°C | Haemaphysalis longicornis | |
36.8 | - |
alpha-keto-gamma-methylthiobutyrate | in 200 mM phosphate buffer, pH7.5, at 37°C | Haemaphysalis longicornis | |
116 | - |
pyruvate | in 200 mM phosphate buffer, pH7.5, at 37°C | Haemaphysalis longicornis | |
315 | - |
3-hydroxykynurenine | in 200 mM phosphate buffer, pH7.5, at 37°C | Haemaphysalis longicornis | |
500 | - |
L-kynurenine | in 200 mM phosphate buffer, pH7.5, at 37°C | Haemaphysalis longicornis |