BRENDA - Enzyme Database show
show all sequences of 2.6.1.5

Biochemical properties and crystal structure of a beta-phenylalanine aminotransferase from Variovorax paradoxus

Crismaru, C.G.; Wybenga, G.G.; Szymanski, W.; Wijma, H.J.; Wu, B.; Bartsch, S.; de Wildeman, S.; Poelarends, G.J.; Feringa, B.L.; Dijkstra, B.W.; Janssen, D.B.; Appl. Environ. Microbiol. 79, 185-195 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinantly expressed in Escherichia coli as a His-tagged fusion protein
Variovorax paradoxus
Crystallization (Commentary)
Crystallization
Organism
the crystal structures of the holoenzyme and of the enzyme in complex with the inhibitor 2-aminooxyacetate reveal structural similarity to the beta-phenylalanine aminotransferase from Mesorhizobium sp. strain LUK
Variovorax paradoxus
Engineering
Amino acid exchange
Commentary
Organism
R41A
mutant is inactive when tested with (S)-beta-phenylalanine as amino donor and 2-oxoglutarate as amino acceptor
Variovorax paradoxus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-aminooxyacetate
-
Variovorax paradoxus
2-oxoglutarate
enzyme shows substrate inhibition
Variovorax paradoxus
beta-phenylalanine
enzyme shows substrate inhibition
Variovorax paradoxus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
2-oxoglutarate
pH 7.6, 30°C
Variovorax paradoxus
1.5
-
(S)-beta-phenylalanine
pH 7.6, 30°C
Variovorax paradoxus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46420
-
calculated from cDNA
Variovorax paradoxus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Variovorax paradoxus
H8WR05
-
-
Purification (Commentary)
Commentary
Organism
using Ni-NTA chromatography
Variovorax paradoxus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-amino-5-methyl-hexanoic acid + 2-oxoglutarate
relative activity: 48%
721359
Variovorax paradoxus
?
-
-
-
?
(S)-beta-phenylalanine + 2-oxoglutarate
relative activity: 100%
721359
Variovorax paradoxus
3-oxo-3-phenylpropionic acid + L-glutamate
-
-
-
?
(S)-beta-phenylalanine + pyruvate
the activity of VpAT with pyruvate is 85% of that with alpha-ketoglutarate as the amino acceptor
721359
Variovorax paradoxus
3-oxo-3-phenylpropionic acid + L-alanine
-
-
-
?
rac-2-fluoro-beta-phenylalanine + 2-oxoglutarate
relative activity: 15%
721359
Variovorax paradoxus
?
-
-
-
?
rac-2-methyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 5%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-bromo-beta-phenylalanine + 2-oxoglutarate
relative activity: 53%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-chloro-beta-phenylalanine + 2-oxoglutarate
relative activity: 56%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-fluoro-beta-phenylalanine + 2-oxoglutarate
relative activity: 48%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-methyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 54%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-bromo-beta-phenylalanine + 2-oxoglutarate
relative activity: 62%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-ethyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 134%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-fluoro-beta-phenylalanine + 2-oxoglutarate
relative activity: 87%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-iso-propyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 61%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-methyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 100%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-nitro-beta-phenylalanine + 2-oxoglutarate
relative activity: 32%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-nitro-hydroxy-phenylalanine + 2-oxoglutarate
relative activity: 61%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-propyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 126%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-trifluoromethyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 73%
721359
Variovorax paradoxus
?
-
-
-
?
rac-beta-leucine + 2-oxoglutarate
relative activity: 38%
721359
Variovorax paradoxus
?
-
-
-
?
rac-beta-phenylalanine + 2-oxoglutarate
relative activity: 100%
721359
Variovorax paradoxus
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Variovorax paradoxus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.6
-
2-oxoglutarate
pH 7.6, 30°C
Variovorax paradoxus
11.8
-
(S)-beta-phenylalanine
pH 7.6, 30°C
Variovorax paradoxus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Variovorax paradoxus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
11.2
enzyme shows high activity over a broad pH range
Variovorax paradoxus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Variovorax paradoxus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
40.3
-
beta-phenylalanine
enzyme shows substrate inhibition, pH 7.6, 30°C
Variovorax paradoxus
82.4
-
2-oxoglutarate
enzyme shows substrate inhibition, pH 7.6, 30°C
Variovorax paradoxus
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinantly expressed in Escherichia coli as a His-tagged fusion protein
Variovorax paradoxus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Variovorax paradoxus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
the crystal structures of the holoenzyme and of the enzyme in complex with the inhibitor 2-aminooxyacetate reveal structural similarity to the beta-phenylalanine aminotransferase from Mesorhizobium sp. strain LUK
Variovorax paradoxus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R41A
mutant is inactive when tested with (S)-beta-phenylalanine as amino donor and 2-oxoglutarate as amino acceptor
Variovorax paradoxus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-aminooxyacetate
-
Variovorax paradoxus
2-oxoglutarate
enzyme shows substrate inhibition
Variovorax paradoxus
beta-phenylalanine
enzyme shows substrate inhibition
Variovorax paradoxus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
40.3
-
beta-phenylalanine
enzyme shows substrate inhibition, pH 7.6, 30°C
Variovorax paradoxus
82.4
-
2-oxoglutarate
enzyme shows substrate inhibition, pH 7.6, 30°C
Variovorax paradoxus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
2-oxoglutarate
pH 7.6, 30°C
Variovorax paradoxus
1.5
-
(S)-beta-phenylalanine
pH 7.6, 30°C
Variovorax paradoxus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46420
-
calculated from cDNA
Variovorax paradoxus
Purification (Commentary) (protein specific)
Commentary
Organism
using Ni-NTA chromatography
Variovorax paradoxus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-amino-5-methyl-hexanoic acid + 2-oxoglutarate
relative activity: 48%
721359
Variovorax paradoxus
?
-
-
-
?
(S)-beta-phenylalanine + 2-oxoglutarate
relative activity: 100%
721359
Variovorax paradoxus
3-oxo-3-phenylpropionic acid + L-glutamate
-
-
-
?
(S)-beta-phenylalanine + pyruvate
the activity of VpAT with pyruvate is 85% of that with alpha-ketoglutarate as the amino acceptor
721359
Variovorax paradoxus
3-oxo-3-phenylpropionic acid + L-alanine
-
-
-
?
rac-2-fluoro-beta-phenylalanine + 2-oxoglutarate
relative activity: 15%
721359
Variovorax paradoxus
?
-
-
-
?
rac-2-methyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 5%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-bromo-beta-phenylalanine + 2-oxoglutarate
relative activity: 53%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-chloro-beta-phenylalanine + 2-oxoglutarate
relative activity: 56%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-fluoro-beta-phenylalanine + 2-oxoglutarate
relative activity: 48%
721359
Variovorax paradoxus
?
-
-
-
?
rac-3-methyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 54%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-bromo-beta-phenylalanine + 2-oxoglutarate
relative activity: 62%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-ethyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 134%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-fluoro-beta-phenylalanine + 2-oxoglutarate
relative activity: 87%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-iso-propyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 61%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-methyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 100%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-nitro-beta-phenylalanine + 2-oxoglutarate
relative activity: 32%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-nitro-hydroxy-phenylalanine + 2-oxoglutarate
relative activity: 61%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-propyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 126%
721359
Variovorax paradoxus
?
-
-
-
?
rac-4-trifluoromethyl-beta-phenylalanine + 2-oxoglutarate
relative activity: 73%
721359
Variovorax paradoxus
?
-
-
-
?
rac-beta-leucine + 2-oxoglutarate
relative activity: 38%
721359
Variovorax paradoxus
?
-
-
-
?
rac-beta-phenylalanine + 2-oxoglutarate
relative activity: 100%
721359
Variovorax paradoxus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Variovorax paradoxus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.6
-
2-oxoglutarate
pH 7.6, 30°C
Variovorax paradoxus
11.8
-
(S)-beta-phenylalanine
pH 7.6, 30°C
Variovorax paradoxus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Variovorax paradoxus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
11.2
enzyme shows high activity over a broad pH range
Variovorax paradoxus
Other publictions for EC 2.6.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739156
Kim
Molecular cloning and characte ...
Scutellaria baicalensis
Nat. Prod. Commun.
9
1311-1314
2014
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4
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721359
Crismaru
Biochemical properties and cry ...
Variovorax paradoxus
Appl. Environ. Microbiol.
79
185-195
2013
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1
1
1
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3
2
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1
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1
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1
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20
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1
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2
1
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2
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1
1
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1
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3
2
2
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20
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2
1
1
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739143
Yoo
An alternative pathway contrib ...
Petunia x hybrida
Nat. Commun.
4
2833
2013
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8
1
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1
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5
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8
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1
1
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7
7
723343
Grossmann
On the mode of action of the h ...
Arabidopsis thaliana
Pest Manag. Sci.
68
482-492
2012
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1
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4
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2
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1
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1
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1
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4
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1
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1
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723432
Riewe
A tyrosine aminotransferase in ...
Arabidopsis thaliana
Plant J.
71
850-859
2012
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3
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1
1
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723465
Lee
Tyrosine aminotransferase cont ...
Papaver somniferum
Plant Physiol.
157
1067-1078
2011
-
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6
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1
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3
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2
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5
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1
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6
1
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6
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1
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2
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5
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1
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6
1
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1
1
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5
5
721558
Prabhu
Identification and partial cha ...
Arabidopsis thaliana
Biochem. Res. Int.
2010
549572
2010
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1
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6
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7
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1
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1
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1
1
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723682
Mehere
Tyrosine aminotransferase: bio ...
Mus musculus
Protein Cell
1
1023-1032
2010
-
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1
1
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7
-
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5
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1
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5
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1
1
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1
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7
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5
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1
1
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7
1
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7
7
705599
Marciano
Functional characterization of ...
Leishmania major
Mol. Biochem. Parasitol.
166
172-182
2009
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1
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1
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6
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1
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3
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1
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1
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1
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1
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3
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706809
Battilana
The 1-deoxy-D: -xylulose 5-pho ...
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
Theor. Appl. Genet.
118
653-669
2009
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2
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2
2
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686608
Rego
Trypanosoma cruzi: characteris ...
Trypanosoma cruzi
Exp. Parasitol.
118
111-117
2008
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1
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1
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702386
Panin
Role of glucocorticoids and re ...
Rattus norvegicus
Biochemistry
73
305-309
2008
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1
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1
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702947
Ebara
Vitamin B12 deficiency results ...
Rattus norvegicus
Br. J. Nutr.
99
503-510
2008
1
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1
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1
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1
-
-
704902
Peragon
Serine dehydratase and tyrosin ...
Oncorhynchus mykiss
J. Exp. Zool. Part A Ecol. Genet. Physiol.
309
25-34
2008
-
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2
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1
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1
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1
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671159
Seetharamappa
Expression, purification, crys ...
Staphylococcus aureus
Acta Crystallogr. Sect. F
F63
452-456
2007
-
-
1
1
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1
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1
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675591
Hazra
Modeling receptor/gene-mediate ...
Rattus norvegicus
J. Pharmacokinet. Pharmacodyn.
34
643-667
2007
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673540
Sivaraman
The narrow substrate specifici ...
Homo sapiens
FEBS J.
273
1920-1929
2006
-
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1
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1
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4
1
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3
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1
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8
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1
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1
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1
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1
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3
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8
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674229
Dundjerski
Mercury influences rat liver t ...
Rattus norvegicus
J. Appl. Toxicol.
26
187-190
2006
1
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2
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1
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675090
Minami-Hori
Richner-Hanhart syndrome: Repo ...
Homo sapiens
J. Dermatol. Sci.
41
82-84
2006
-
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1
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1
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1
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1
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1
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-
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676025
Charfeddine
Clinical and mutational invest ...
Homo sapiens
Mol. Genet. Metab.
88
184-191
2006
-
-
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2
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1
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2
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2
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1
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1
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2
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1
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-
657707
Johansson
Xenobiotics and the glucocorti ...
Rattus norvegicus
Basic Clin. Pharmacol. Toxicol.
96
309-315
2005
-
-
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4
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3
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2
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-
675646
Hollaender-Czytko
Tocopherol content and activit ...
Arabidopsis thaliana
J. Plant Physiol.
162
767-770
2005
-
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4
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1
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1
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1
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-
-
-
657704
Amin
-
Purification and properties of ...
Entodinium caudatum
Aust. J. Agric. Res.
55
991-997
2004
-
-
-
-
-
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8
-
1
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1
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1
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1
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1
1
1
1
-
1
1
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8
-
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1
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1
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1
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-
-
-
-
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
658409
Rehman
Tyrosine aminotransferase and ...
Homo sapiens
Cell Biochem. Funct.
22
89-96
2004
-
-
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-
-
-
-
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2
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5
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5
-
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-
-
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-
-
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-
-
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-
-
-
660463
Rothman
Directed evolution relieves pr ...
Escherichia coli
Protein Sci.
13
763-772
2004
-
-
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1
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3
-
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2
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1
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4
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1
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3
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1
-
-
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-
4
-
-
-
-
-
-
-
-
-
-
660454
Sobrado
Involvement of conserved aspar ...
Rattus norvegicus, Trypanosoma cruzi
Protein Sci.
12
1039-1050
2003
-
-
-
-
7
-
-
24
-
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-
2
-
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5
-
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27
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7
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24
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5
-
-
-
-
27
-
-
-
-
-
-
-
-
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-
640126
Tabiri
Hepatic tyrosine aminotransfer ...
Gallus gallus
Br. Poult. Sci.
43
629-634
2002
-
-
-
-
-
-
-
-
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-
2
-
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1
1
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1
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640124
Blankenfeldt
Crystal structure of Trypanoso ...
Trypanosoma cruzi
Protein Sci.
8
2406-2417
1999
-
-
-
1
-
-
-
-
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2
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1
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1
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1
1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640125
Ko
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
55
1474-1477
1999
-
-
-
1
-
-
-
-
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3
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640120
Bai
Vitamin B-6 deficiency and lev ...
Felis catus
J. Nutr.
128
1995-2000
1998
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
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-
1
1
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-
-
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1
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1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640121
Nimi
Effect of dexamethasone pretre ...
Rattus norvegicus
Biol. Pharm. Bull.
21
1009-1012
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
2
1
-
1
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-
-
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-
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-
-
-
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-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640122
Pickering
Rat primary hepatocytes and H4 ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
252
764-769
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
1
-
1
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-
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-
-
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-
-
-
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-
-
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-
-
-
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-
3
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640123
Donohue
Contrasting effects of acute a ...
Rattus norvegicus
Alcohol
15
141-146
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640119
Presch
The effect of estradiol and ov ...
Rattus norvegicus
Life Sci.
60
479-484
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640118
Montemartini
Purification and partial struc ...
Trypanosoma cruzi
Biochem. J.
292
901-906
1993
-
-
-
-
-
-
-
6
-
-
2
-
-
4
-
1
1
-
-
2
1
-
2
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
2
-
-
-
1
1
-
2
1
-
2
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
640112
Dietrich
Expression of mammalian tyrosi ...
Rattus norvegicus
Eur. J. Biochem.
201
399-407
1991
-
-
1
-
-
1
1
3
-
-
4
-
-
5
-
-
1
-
-
4
2
-
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
3
-
-
4
-
-
-
-
1
-
4
2
-
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
640111
De-Eknamkul
Purification and characterizat ...
Anchusa officinalis
Arch. Biochem. Biophys.
257
430-438
1987
-
-
-
-
-
-
2
3
-
-
4
1
-
2
-
-
1
-
-
2
1
-
5
1
1
-
3
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
4
1
-
-
-
1
-
2
1
-
5
1
1
-
3
-
2
1
-
-
-
-
-
-
-
-
640114
Rege
Purification and characterizat ...
Crithidia fasciculata
Mol. Biochem. Parasitol.
25
1-9
1987
-
-
-
-
-
-
-
-
-
-
3
-
-
5
-
1
1
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640115
Echetebu
Hepatic tyrosine aminotransfer ...
Agama agama
Biochimie
69
223-230
1987
-
-
-
-
-
3
6
1
1
-
1
-
-
2
-
-
1
-
-
2
1
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
3
-
6
1
1
1
-
1
-
-
-
-
1
-
2
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
640110
Echetebu
-
Some properties of tyrosine am ...
Trichoderma viride
J. Gen. Microbiol.
128
2735-2738
1982
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
640101
Hargrove
Physical properties, limited p ...
Rattus norvegicus
J. Biol. Chem.
256
8012-8017
1981
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640116
Belew
-
Tyrosine aminotransferase from ...
Drosophila hydei
Insect Biochem.
11
239-245
1981
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
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-
30141
Gohda
Purification and characterizat ...
Rattus norvegicus
J. Biol. Chem.
255
7371-7379
1980
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2
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1
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2
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1
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2
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1
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640099
Hargrove
Purification of the native for ...
Rattus norvegicus
Anal. Biochem.
104
231-235
1980
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2
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1
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1
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1
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2
1
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1
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640100
Lee
Properties of tyrosine aminotr ...
Rattus norvegicus
Anal. Biochem.
95
188-193
1979
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3
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2
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1
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2
1
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1
1
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1
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1
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3
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1
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2
1
-
1
1
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640102
Donner
Tyrosine aminotransferase from ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
80
766-772
1978
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1
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2
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1
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2
1
1
1
1
1
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1
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1
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2
1
1
1
1
1
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640104
Belarbi
Purification and characterizat ...
Rattus norvegicus
FEBS Lett.
75
221-225
1977
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1
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2
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2
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1
1
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2
1
1
1
1
1
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1
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2
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1
1
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2
1
1
1
1
1
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640105
Roewekamp
Purification and subunit struc ...
Rattus norvegicus
FEBS Lett.
73
225-228
1977
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2
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2
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2
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1
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2
1
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1
1
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2
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2
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1
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2
1
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1
1
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640113
Ohisalo
Partial purification and prope ...
Rana temporaria
Biochem. J.
163
411-417
1977
4
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3
1
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1
1
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2
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1
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2
1
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3
-
1
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4
-
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3
-
1
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1
1
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1
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2
1
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3
-
1
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640117
Ohisalo
Heterogeneity of hepatic tyros ...
Rana temporaria, Rattus norvegicus
Acta Chem. Scand. B
30
491-500
1976
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2
3
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3
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2
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4
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2
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2
3
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2
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4
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2
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640103
Iwasaki
Studies on the induction and r ...
Rattus norvegicus
Eur. J. Biochem.
34
347-357
1973
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-
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1
5
-
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1
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2
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1
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4
1
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3
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1
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1
4
-
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1
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1
4
5
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1
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1
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4
1
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3
-
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1
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640107
Johnson
Regulation of tyrosine aminotr ...
Rattus norvegicus
J. Biol. Chem.
248
4521-4527
1973
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1
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2
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2
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1
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1
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2
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1
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640108
Miller
Purification of tyrosine amino ...
Rattus norvegicus
Biochim. Biophys. Acta
276
407-415
1972
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1
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1
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1
2
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1
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1
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1
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1
2
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1
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1
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640098
Miller
Purification, properties, and ...
Rattus norvegicus
J. Biol. Chem.
246
3234-3240
1971
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1
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4
-
1
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2
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1
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1
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1
1
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14
-
1
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1
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4
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1
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2
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1
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1
1
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14
-
1
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640109
Jacoby
Studies on the specificity of ...
Rattus norvegicus
J. Biol. Chem.
239
419-424
1964
-
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-
23
4
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1
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1
1
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9
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23
-
4
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1
1
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9
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