BRENDA - Enzyme Database show
show all sequences of 2.6.1.43

A rapid, high-yield purification of L-alanine:4,5-dioxovalerate transaminase from rat kidney mitochondria using an improved enzyme assay method

Tyagi, R.K.; Datta, K.; Protein Expr. Purif. 5, 527-533 (1994)

Data extracted from this reference:

General Stability
General Stability
Organism
repeated freezing and thawing causes inactivation
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
hemin
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
mitochondrial matrix
Rattus norvegicus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
210000
-
gel filtration, gradient polyacrylamide gel electrophoresis
Rattus norvegicus
225000
-
gel filtration, Sepharose 6B
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
Wistar
-
Rattus norvegicus Wistar
-
Wistar
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36.9
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, can be stored for nearly 1 year without significant loss of enzyme activity
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
Subunits
Subunits
Commentary
Organism
tetramer
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Rattus norvegicus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
3fold increase of activity from 37°C to 60°C
Rattus norvegicus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
65
retains more than 90% maximum activity through 55-65°C, beyond 65°C activity decreases sharply
Rattus norvegicus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
isoelectric focusing analysis, 2-dimensional gel electrophoresis
-
5
General Stability (protein specific)
General Stability
Organism
repeated freezing and thawing causes inactivation
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hemin
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
mitochondrial matrix
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
210000
-
gel filtration, gradient polyacrylamide gel electrophoresis
Rattus norvegicus
225000
-
gel filtration, Sepharose 6B
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
alternate route for delta-aminolevulinate formation
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
L-alanine + 4,5-dioxopentanoate
Rattus norvegicus Wistar
heme biosynthetic pathway
5-aminolevulinate + pyruvate
-
Rattus norvegicus Wistar
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36.9
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, can be stored for nearly 1 year without significant loss of enzyme activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus
ir
L-alanine + 4,5-dioxopentanoate
-
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
L-alanine + 4,5-dioxopentanoate
alternate route for delta-aminolevulinate formation
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
L-alanine + 4,5-dioxopentanoate
heme biosynthetic pathway
640062
Rattus norvegicus Wistar
5-aminolevulinate + pyruvate
-
640062
Rattus norvegicus Wistar
ir
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 50000, mitochondrial isozyme, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Rattus norvegicus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
3fold increase of activity from 37°C to 60°C
Rattus norvegicus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
65
retains more than 90% maximum activity through 55-65°C, beyond 65°C activity decreases sharply
Rattus norvegicus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
isoelectric focusing analysis, 2-dimensional gel electrophoresis
-
5
Other publictions for EC 2.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739231
Salgueiro
Effects of Bauhinia forficata ...
Mus musculus
Oxid. Med. Cell. Longev.
2016
0000
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640064
Lombardo
5-Aminolevulinic acid synthesi ...
Trypanosoma cruzi
Int. J. Biochem. Cell Biol.
35
1263-1271
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640063
Sagar
L-alanine: 4,5-dioxovalerate t ...
Leishmania donovani
Microbiol. Res.
150
419-423
1995
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
640062
Tyagi
A rapid, high-yield purificati ...
Rattus norvegicus, Rattus norvegicus Wistar
Protein Expr. Purif.
5
527-533
1994
-
-
-
-
-
1
1
-
1
-
3
4
-
166
-
-
1
-
-
2
1
1
6
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
3
4
-
-
-
1
-
2
1
1
6
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
640061
Tyagi
In vitro translocation of L-al ...
Rattus norvegicus
J. Biochem.
113
557-562
1993
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640059
McKinney
Production of delta-aminolevul ...
Mus musculus
Int. J. Biochem.
23
803-810
1991
-
-
-
-
-
-
7
2
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640060
Singh
Cytosolic L-alanine:4,5-dioxov ...
Rattus norvegicus, Rattus norvegicus Wistar
Eur. J. Biochem.
198
581-587
1991
1
-
-
-
-
1
1
-
2
-
4
-
-
166
-
-
1
-
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
4
-
-
-
-
1
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640057
Hoare
Characteristics of L-alanine:4 ...
Candida albicans, Candida albicans 3100, Saccharomyces cerevisiae, Saccharomyces cerevisiae 3059 S288C
Arch. Biochem. Biophys.
277
122-129
1990
-
-
-
-
-
1
3
2
2
-
4
4
-
8
-
-
2
-
-
-
1
1
16
2
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
1
-
3
-
2
2
-
4
4
-
-
-
2
-
-
1
1
16
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
640058
McKinney
Production of delta-aminolevul ...
Mus musculus, Mus musculus C57/BL6J
Int. J. Biochem.
22
347-357
1990
-
-
-
-
-
-
-
-
2
-
1
2
-
63
-
-
1
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640055
Ades
Synthetic substrates for throm ...
Gallus gallus
Int. J. Biochem.
21
579-587
1989
-
-
-
-
-
-
-
2
2
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640056
Prasad
-
Characterization of L-alanine: ...
Cenchrus americanus
Biochem. Int.
18
149-161
1989
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640054
Rhee
L-alanine: 4,5-dioxovalerate a ...
Devosia riboflavina
J. Biochem.
103
1045-1049
1988
-
-
-
1
-
-
3
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
7
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
2
-
-
2
1
-
-
-
1
-
-
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640053
Shanker
Affinity purification and prop ...
Rattus norvegicus, Rattus norvegicus Wistar
Arch. Biochem. Biophys.
248
652-657
1986
-
-
-
-
-
1
7
2
1
-
2
2
-
166
-
-
1
-
-
1
1
3
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
7
-
2
1
-
2
2
-
-
-
1
-
1
1
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640051
Shanker
Evidence of hemin as an end pr ...
Rattus norvegicus, Rattus norvegicus Wistar
FEBS Lett.
189
129-132
1985
-
-
-
-
-
-
1
-
1
-
-
2
-
166
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640052
Singh
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
827
305-309
1985
-
-
-
-
-
-
2
2
-
-
2
1
-
1
-
-
1
-
-
1
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
1
-
-
-
1
-
1
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640049
Shioi
-
Purification and characterizat ...
Raphanus sativus
Plant Cell Physiol.
25
1487-1493
1984
-
-
-
-
-
-
1
2
-
-
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1
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1
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1
2
12
1
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1
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1
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1
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6
1
4
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2
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1
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1
2
12
1
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1
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1
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1
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1
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5
1
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9
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1
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5
1
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3
1
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1
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1
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1
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3
1
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1
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1
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11
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1
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1
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1
1
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1
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6
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2
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1
1
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1
1
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11
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1
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25
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