BRENDA - Enzyme Database show
show all sequences of 2.6.1.43

L-alanine: 4,5-dioxovalerate aminotransferase from Pseudomonas riboflavina: purification and inactivation by methylglyoxal

Rhee, H.i.; Murata, K.; Kimura, A.; J. Biochem. 103, 1045-1049 (1988)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
crystallized as bright yellow needles
Devosia riboflavina
Inhibitors
Inhibitors
Commentary
Organism
Structure
hemin
-
Devosia riboflavina
methylglyoxal
-
Devosia riboflavina
pyruvate
-
Devosia riboflavina
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.75
-
4,5-Dioxopentanoate
pH 8.0, 37°C
Devosia riboflavina
1.7
-
L-alanine
pH 8.0, 37°C
Devosia riboflavina
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
4 * 48000, SDS-PAGE
Devosia riboflavina
190000
-
gel filtration
Devosia riboflavina
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Devosia riboflavina
delta-aminolevulinate pathway
5-aminolevulinate + pyruvate
-
Devosia riboflavina
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Devosia riboflavina
-
IFO 3140
-
Purification (Commentary)
Commentary
Organism
-
Devosia riboflavina
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.02
-
-
Devosia riboflavina
Storage Stability
Storage Stability
Organism
-15°C, stable for 3 months
Devosia riboflavina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + 4,5-dioxopentanoate
39% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate
34% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
55% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
L-alanine + 4,5-dioxopentanoate
delta-aminolevulinate pathway
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
L-ornithine + 4,5-dioxopentanoate
14% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
additional information
L-lysine, L-arginine, L-glutamate, L-methionine, L-histidine, glycine, L-phenylalanine, L-threonine, L-asparagine, L-serine, L-proline, L-valine, L-isoleucine, L-leucine, L-tyrosine, L-cysteine and L-tryptophan are all inert as amino donors, methylglyoxal is a poor substrate for the enzyme
640054
Devosia riboflavina
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 48000, SDS-PAGE
Devosia riboflavina
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Devosia riboflavina
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Devosia riboflavina
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystallized as bright yellow needles
Devosia riboflavina
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hemin
-
Devosia riboflavina
methylglyoxal
-
Devosia riboflavina
pyruvate
-
Devosia riboflavina
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.75
-
4,5-Dioxopentanoate
pH 8.0, 37°C
Devosia riboflavina
1.7
-
L-alanine
pH 8.0, 37°C
Devosia riboflavina
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
4 * 48000, SDS-PAGE
Devosia riboflavina
190000
-
gel filtration
Devosia riboflavina
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Devosia riboflavina
delta-aminolevulinate pathway
5-aminolevulinate + pyruvate
-
Devosia riboflavina
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Devosia riboflavina
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.02
-
-
Devosia riboflavina
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, stable for 3 months
Devosia riboflavina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + 4,5-dioxopentanoate
39% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate
34% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
55% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
L-alanine + 4,5-dioxopentanoate
delta-aminolevulinate pathway
640054
Devosia riboflavina
5-aminolevulinate + pyruvate
-
640054
Devosia riboflavina
ir
L-ornithine + 4,5-dioxopentanoate
14% of the activity with L-alanine
640054
Devosia riboflavina
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
additional information
L-lysine, L-arginine, L-glutamate, L-methionine, L-histidine, glycine, L-phenylalanine, L-threonine, L-asparagine, L-serine, L-proline, L-valine, L-isoleucine, L-leucine, L-tyrosine, L-cysteine and L-tryptophan are all inert as amino donors, methylglyoxal is a poor substrate for the enzyme
640054
Devosia riboflavina
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 48000, SDS-PAGE
Devosia riboflavina
Other publictions for EC 2.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739231
Salgueiro
Effects of Bauhinia forficata ...
Mus musculus
Oxid. Med. Cell. Longev.
2016
0000
2016
-
1
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
640064
Lombardo
5-Aminolevulinic acid synthesi ...
Trypanosoma cruzi
Int. J. Biochem. Cell Biol.
35
1263-1271
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
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-
-
-
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1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640063
Sagar
L-alanine: 4,5-dioxovalerate t ...
Leishmania donovani
Microbiol. Res.
150
419-423
1995
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
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7
-
1
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1
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-
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2
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1
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1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
640062
Tyagi
A rapid, high-yield purificati ...
Rattus norvegicus, Rattus norvegicus Wistar
Protein Expr. Purif.
5
527-533
1994
-
-
-
-
-
1
1
-
1
-
3
4
-
166
-
-
1
-
-
2
1
1
6
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
3
4
-
-
-
1
-
2
1
1
6
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
640061
Tyagi
In vitro translocation of L-al ...
Rattus norvegicus
J. Biochem.
113
557-562
1993
-
-
-
-
-
-
1
-
1
-
-
1
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1
-
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1
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2
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1
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1
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1
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640059
McKinney
Production of delta-aminolevul ...
Mus musculus
Int. J. Biochem.
23
803-810
1991
-
-
-
-
-
-
7
2
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640060
Singh
Cytosolic L-alanine:4,5-dioxov ...
Rattus norvegicus, Rattus norvegicus Wistar
Eur. J. Biochem.
198
581-587
1991
1
-
-
-
-
1
1
-
2
-
4
-
-
166
-
-
1
-
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
4
-
-
-
-
1
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640057
Hoare
Characteristics of L-alanine:4 ...
Candida albicans, Candida albicans 3100, Saccharomyces cerevisiae, Saccharomyces cerevisiae 3059 S288C
Arch. Biochem. Biophys.
277
122-129
1990
-
-
-
-
-
1
3
2
2
-
4
4
-
8
-
-
2
-
-
-
1
1
16
2
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
1
-
3
-
2
2
-
4
4
-
-
-
2
-
-
1
1
16
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
640058
McKinney
Production of delta-aminolevul ...
Mus musculus, Mus musculus C57/BL6J
Int. J. Biochem.
22
347-357
1990
-
-
-
-
-
-
-
-
2
-
1
2
-
63
-
-
1
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
2
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1
2
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-
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1
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640055
Ades
Synthetic substrates for throm ...
Gallus gallus
Int. J. Biochem.
21
579-587
1989
-
-
-
-
-
-
-
2
2
-
1
1
-
1
-
-
1
-
-
2
-
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1
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-
-
-
-
-
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2
2
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1
1
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1
-
2
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640056
Prasad
-
Characterization of L-alanine: ...
Cenchrus americanus
Biochem. Int.
18
149-161
1989
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
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1
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1
1
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3
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2
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2
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1
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1
1
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-
-
-
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-
-
-
-
-
-
-
-
-
640054
Rhee
L-alanine: 4,5-dioxovalerate a ...
Devosia riboflavina
J. Biochem.
103
1045-1049
1988
-
-
-
1
-
-
3
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
7
1
-
-
-
-
-
-
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1
-
-
-
-
-
-
1
1
-
-
-
3
-
2
-
-
2
1
-
-
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1
-
-
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640053
Shanker
Affinity purification and prop ...
Rattus norvegicus, Rattus norvegicus Wistar
Arch. Biochem. Biophys.
248
652-657
1986
-
-
-
-
-
1
7
2
1
-
2
2
-
166
-
-
1
-
-
1
1
3
4
1
-
-
-
-
-
-
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1
-
-
-
-
-
-
1
-
-
1
-
7
-
2
1
-
2
2
-
-
-
1
-
1
1
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640051
Shanker
Evidence of hemin as an end pr ...
Rattus norvegicus, Rattus norvegicus Wistar
FEBS Lett.
189
129-132
1985
-
-
-
-
-
-
1
-
1
-
-
2
-
166
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
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2
-
-
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-
-
-
-
-
-
-
1
2
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1
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640052
Singh
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
827
305-309
1985
-
-
-
-
-
-
2
2
-
-
2
1
-
2
-
-
1
-
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
1
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1
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640049
Shioi
-
Purification and characterizat ...
Raphanus sativus
Plant Cell Physiol.
25
1487-1493
1984
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640050
Shioi
Purification and properties of ...
Chlorella regularis, Chlorella regularis S-50
Arch. Biochem. Biophys.
234
117-124
1984
-
-
-
-
-
1
6
4
-
-
2
2
-
7
-
-
1
-
-
-
1
2
12
1
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
6
1
4
-
-
2
2
-
-
-
1
-
-
1
2
12
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
640048
Bajkowski
delta-Aminolevulinic acid form ...
Clostridium tetanomorphum
J. Biol. Chem.
257
2207-2211
1982
-
-
-
-
-
-
9
2
-
-
5
1
-
3
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
2
-
-
5
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640047
Noguchi
Biosynthesis of porphyrin prec ...
Bos taurus
J. Biol. Chem.
256
10335-10339
1981
-
-
-
-
-
-
5
4
1
-
4
1
-
1
-
-
1
-
-
1
1
2
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
4
1
-
4
1
-
-
-
1
-
1
1
2
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
640046
Varticovski
Biosynthesis of porphyrin prec ...
Bos taurus
J. Biol. Chem.
255
3742-3747
1980
-
-
-
1
-
1
6
2
2
-
1
1
-
1
-
-
1
-
-
1
1
-
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
1
-
6
-
2
2
-
1
1
-
-
-
1
-
1
1
-
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640045
Neuberger
gamma, delta-Dioxovalerate ami ...
Rhodobacter sphaeroides
Biochim. Biophys. Acta
67
342-345
1963
-
-
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1
25
2
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1
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2
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1
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1
7
-
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-
-
1
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-
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1
-
25
-
2
-
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1
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1
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1
7
-
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1
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