Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Volvariella volvacea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-2-deoxy-D-glucitol-6-phosphate | - |
Volvariella volvacea | |
N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid | - |
Volvariella volvacea | |
uridine 5'-diphospho-N-acetylglucosamine | - |
Volvariella volvacea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
D-fructose 6-phosphate | recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol | Volvariella volvacea | |
0.75 | - |
L-glutamine | recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol | Volvariella volvacea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
4 * 77000, SDS-PAGE | Volvariella volvacea |
306000 | - |
estimated from amino acid sequence | Volvariella volvacea |
310000 | - |
gel filtration | Volvariella volvacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Volvariella volvacea | Q6DLZ8 | - |
- |
Volvariella volvacea V1-1 | Q6DLZ8 | - |
- |
Purification (Comment) | Organism |
---|---|
partial purification by immobilized Ni2+ affinity column chromatography | Volvariella volvacea |
Storage Stability | Organism |
---|---|
4°C, 50 mM MOPS (pH 6.5), 1 mM dithiothreitol, 1 mM EDTA, and 10 mM KCl, overnight storage, recombinant enzyme activity is low and no longer evident with preparations losing 60% of their activity | Volvariella volvacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Volvariella volvacea | L-glutamate + D-glucosamine 6-phosphate | - |
? | |
L-glutamine + D-fructose 6-phosphate | - |
Volvariella volvacea V1-1 | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 77000, SDS-PAGE | Volvariella volvacea |
Synonyms | Comment | Organism |
---|---|---|
GFAT | - |
Volvariella volvacea |
GlcN-6-P synthase | - |
Volvariella volvacea |
glucosamine-6-phosphate synthase | - |
Volvariella volvacea |
L-glutamine D-fructose 6-phosphate amidotransferase | - |
Volvariella volvacea |
L-glutamine:D-fructose-6-phosphate amidotransferase (hexose-isomerizing) | - |
Volvariella volvacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Volvariella volvacea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life of 1 h at 45°C | Volvariella volvacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Volvariella volvacea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | - |
Volvariella volvacea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.085 | - |
recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol | Volvariella volvacea | 2-amino-2-deoxy-D-glucitol-6-phosphate | |
0.12 | - |
recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol | Volvariella volvacea | N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid | |
0.16 | - |
recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol | Volvariella volvacea | uridine 5'-diphospho-N-acetylglucosamine |