BRENDA - Enzyme Database
show all sequences of 2.6.1.15

Glutamine transaminase L from rat liver

Cooper, A.J.L.; Meister, A.; Methods Enzymol. 113, 338-343 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Aminoxyacetate
soluble fraction
Rattus norvegicus
cycloserine
soluble fraction
Rattus norvegicus
hydroxylamine
soluble fraction
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
-
Rattus norvegicus
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
male Sprague-Dawley
-
Purification (Commentary)
Purification (Commentary)
Organism
DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.113
-
mitochondrion
Rattus norvegicus
5
-
soluble fraction
Rattus norvegicus
Storage Stability
Storage Stability
Organism
4ºC, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glyoxylate + L-glutamine
best substrate
639897
Rattus norvegicus
glycine + 2-oxoglutaramate
-
639897
Rattus norvegicus
?
glyoxylate + L-methionine
-
639897
Rattus norvegicus
glycine + 4-methylsulfanyl-2-oxobutanoate
-
639897
Rattus norvegicus
?
L-glutamine + 2-oxoglutaramate
-
639897
Rattus norvegicus
2-oxoglutaramate + L-glutamine
-
639897
Rattus norvegicus
?
L-glutamine + 3-mercaptopyruvate
-
639897
Rattus norvegicus
2-oxoglutaramate + L-cysteine
-
639897
Rattus norvegicus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Aminoxyacetate
soluble fraction
Rattus norvegicus
cycloserine
soluble fraction
Rattus norvegicus
hydroxylamine
soluble fraction
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
-
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.113
-
mitochondrion
Rattus norvegicus
5
-
soluble fraction
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
4ºC, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glyoxylate + L-glutamine
best substrate
639897
Rattus norvegicus
glycine + 2-oxoglutaramate
-
639897
Rattus norvegicus
?
glyoxylate + L-methionine
-
639897
Rattus norvegicus
glycine + 4-methylsulfanyl-2-oxobutanoate
-
639897
Rattus norvegicus
?
L-glutamine + 2-oxoglutaramate
-
639897
Rattus norvegicus
2-oxoglutaramate + L-glutamine
-
639897
Rattus norvegicus
?
L-glutamine + 3-mercaptopyruvate
-
639897
Rattus norvegicus
2-oxoglutaramate + L-cysteine
-
639897
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Other publictions for EC 2.6.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636642
Mosca
Tissue expression and translat ...
Rattus norvegicus
Biochim. Biophys. Acta
1628
1-10
2003
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-
1
-
-
-
-
-
2
-
1
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-
2
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-
-
-
-
2
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639903
Duran
-
Role of the glutamine transami ...
Rhizobium etli
Microbiology
141
589-595
1995
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-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
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-
1
-
1
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-
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-
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-
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-
-
-
-
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-
-
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-
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-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639902
Coccia
Transamination of some sulphur ...
Bos taurus
Physiol. Chem. Phys. Med. NMR
24
313-321
1992
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-
-
-
-
-
-
-
-
-
-
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2
-
-
-
-
-
2
-
-
2
-
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-
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2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639897
Cooper
Glutamine transaminase L from ...
Rattus norvegicus
Methods Enzymol.
113
338-343
1985
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-
-
-
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3
-
2
-
1
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-
2
-
-
1
-
-
2
2
1
4
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
1
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-
-
-
1
-
2
2
1
4
-
1
-
-
-
-
-
-
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-
-
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-
-
-
639901
Cooper
Isolation and properties of a ...
Rattus norvegicus
J. Biol. Chem.
249
2554-2561
1974
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
1
-
-
2
1
-
21
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
2
1
-
21
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
391804
Cooper
Action of liver glutamine tran ...
Rattus norvegicus
J. Biol. Chem.
248
8499-8505
1973
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-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
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-
1
1
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3
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-
1
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-
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-
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-
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-
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-
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-
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1
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
639899
Cooper
Activity of liver glutamine tr ...
Rattus norvegicus
J. Biol. Chem.
248
8489-8498
1973
-
-
-
-
-
-
4
2
-
-
-
-
-
1
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
1
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
639898
Cooper
Isolation and properties of hi ...
Rattus norvegicus
Biochemistry
11
661-671
1972
-
-
-
-
-
1
6
10
-
-
1
2
-
1
-
-
1
-
-
1
1
4
40
-
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
10
-
-
1
2
-
-
-
1
-
1
1
4
40
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-