Cloned (Comment) | Organism |
---|---|
hexahistidine-tagged octaprenyl diphosphate synthase | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
dimethylallyl diphosphate | at pH 7.5 and 30°C | Escherichia coli | |
0.34 | - |
isopentenyl diphosphate | with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Escherichia coli | - |
5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Escherichia coli K12 | - |
5 diphosphate + all-trans-octaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AD57 | - |
- |
Escherichia coli | P0AD57 | K12 strain SF5 | - |
Escherichia coli K12 | P0AD57 | K12 strain SF5 | - |
Purification (Comment) | Organism |
---|---|
hexahistidine-tagged octaprenyl diphosphate synthase | Escherichia coli |
Ni-NTA agarose column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
430 | - |
with dimethylallyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
3100 | - |
with geranyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
31000 | - |
with geranylgeranyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
58000 | - |
with (2E,6E)-farnesyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | (2E,6E)-farnesyl diphosphate is the most effective substrate | Escherichia coli | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli K12 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | (2E,6E)-farnesyl diphosphate is the most effective substrate | Escherichia coli K12 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
dimethylallyl diphosphate + isopentenyl diphosphate | 0.7% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli | diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate | - |
? | |
dimethylallyl diphosphate + isopentenyl diphosphate | 0.7% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli K12 | diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | 5.3% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli | ? | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | 5.3% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli K12 | ? | - |
? | |
geranylgeranyl diphosphate + isopentenyl diphosphate | 53.4% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli | ? | - |
? | |
geranylgeranyl diphosphate + isopentenyl diphosphate | 53.4% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli K12 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
IspB | - |
Escherichia coli |
octaprenyl diphosphate synthase | - |
Escherichia coli |