Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Thermotoga maritima | |
0.002 | - |
isopentenyl diphosphate | pH 7.5, 25°C | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Thermotoga maritima | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Thermotoga maritima DSM 3109 | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X1M1 | - |
- |
Thermotoga maritima DSM 3109 | Q9X1M1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | Thermotoga maritima | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the product of chain elongation catalyzed by the Thermotoga maritima enzyme is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM octaprenyl pyrophosphate synthase/farnesyl pyrophosphate complex and 0.001 mM isopentenyl pyrophosphate, only the C20 is formed | Thermotoga maritima | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the polymer all-trans C40-octaprenyl pyrophosphate forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | Thermotoga maritima DSM 3109 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the product of chain elongation catalyzed by the Thermotoga maritima enzyme is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM octaprenyl pyrophosphate synthase/farnesyl pyrophosphate complex and 0.001 mM isopentenyl pyrophosphate, only the C20 is formed | Thermotoga maritima DSM 3109 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
octaprenyl pyrophosphate synthase | - |
Thermotoga maritima |
OPPs | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 85 | the enzyme activity at temperatures ranging from 25°C to 85°C exponentially increases with elevated temperature | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
Tm-value is above 80°C | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Thermotoga maritima | |
0.005 | - |
isopentenyl diphosphate | pH 7.5, 25°C | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
metabolism | octaprenyl pyrophosphate synthase catalyzes the chain elongation of farnesyl pyrophosphate via consecutive condensation reactions with five molecules of isopentenyl pyrophosphate to generate all-trans C40-octaprenyl pyrophosphate. The polymer forms the side chain of ubiquinone that is involved in electron transport system to produce ATP | Thermotoga maritima |