Literature summary for 2.5.1.85 extracted from

Ferella, M.; Montalvetti, A.; Rohloff, P.; Miranda, K.; Fang, J.; Reina, S.; Kawamukai, M.; Bua, J.; Nilsson, D.; Pravia, C.; Katzin, A.; Cassera, M.B.; Aslund, L.; Andersson, B.; Docampo, R.; Bontempi, E.J.
A solanesyl-diphosphate synthase localizes in glycosomes of Trypanosoma cruzi (2006), J. Biol. Chem., 281, 39339-39348.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of TcSPPS is able to complement an Escherichia coli ispB mutant	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00459	-	farnesyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi
0.00707	-	geranylgeranyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi
0.0192	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: geranyl diphosphate	Trypanosoma cruzi
0.0243	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate	Trypanosoma cruzi
0.0307	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: farnesyl diphosphate	Trypanosoma cruzi
0.0548	-	geranyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
glycosome	-	Trypanosoma cruzi	20015	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	optimal level of activity obtained at 0.5-1 mM. No activation by Mn2+	Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	x * 39000, calculated from sequence	Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranylgeranyl diphosphate + 5 isopentenyl diphosphate	Trypanosoma cruzi	the enzyme is involved in synthesis of ubiquinone-9	5 diphosphate + all-trans-nonaprenyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q964Q8	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Trypanosoma cruzi

Source Tissue

Source Tissue	Comment	Organism	Textmining
epimastigote	-	Trypanosoma cruzi	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate	-	Trypanosoma cruzi	6 diphosphate + all-trans-nonaprenyl diphosphate	-	?
dimethylallyl diphosphate + 8 isopentenyl diphosphate	low activity	Trypanosoma cruzi	8 diphosphate + all-trans-nonaprenyl diphosphate	-	?
geranyl diphosphate + 7 isopentenyl diphosphate	-	Trypanosoma cruzi	7 diphosphate + all-trans-nonaprenyl diphosphate	-	?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate	geranylgeranyl diphosphate is the preferred allylic substrate	Trypanosoma cruzi	5 diphosphate + all-trans-nonaprenyl diphosphate	-	?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate	the enzyme is involved in synthesis of ubiquinone-9	Trypanosoma cruzi	5 diphosphate + all-trans-nonaprenyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 39000, calculated from sequence	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
solanesyl-diphosphate synthase	-	Trypanosoma cruzi
SPPS	-	Trypanosoma cruzi
TCSPPS	-	Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.032	-	(2E,6E)-farnesyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi
0.043	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: farnesyl diphosphate	Trypanosoma cruzi
0.065	-	geranyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi
0.087	-	geranylgeranyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi
0.131	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: geranyl diphosphate	Trypanosoma cruzi
0.217	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate	Trypanosoma cruzi

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trypanosoma cruzi	calculated from sequence	-	6.01

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00119	-	geranyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi
0.0014	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: farnesyl diphosphate	Trypanosoma cruzi
0.00685	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: geranyl diphosphate	Trypanosoma cruzi
0.00701	-	(2E,6E)-farnesyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi
0.00893	-	isopentenyl diphosphate	pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate	Trypanosoma cruzi
0.012	-	geranylgeranyl diphosphate	pH 7.4, 37°C	Trypanosoma cruzi

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Release 2023.1 (January 2023)