Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermococcus kodakarensis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 22% (w/v) polyethylene glycol 3350 and 0.2 M sodium citrate | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
L-methionine | at pH 8.0 and 37°C | Thermococcus kodakarensis | |
6.54 | - |
ATP | at pH 8.0 and 37°C | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44300 | - |
- |
Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Thermococcus kodakarensis | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Thermococcus kodakarensis | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000-55000, SDS-PAGE | Thermococcus kodakarensis |
homodimer | 2 * 44300, calculated from amino acid sequence | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
MAT | - |
Thermococcus kodakarensis |
methionine adenosyltransferase | - |
Thermococcus kodakarensis |
MetK | - |
Thermococcus kodakarensis |
S-adenosylmethionine synthase | - |
Thermococcus kodakarensis |