BRENDA - Enzyme Database
show all sequences of 2.5.1.49

Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product

Shimizu, H.; Yamagata, S.; Masui, R.; Inoue,Y.; Shibata, T.; Yokoyama, S.; Kuramitsu, S.; Iwama, T.; Biochim. Biophys. Acta 1549, 61-72 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
oah1 gene cloned, sequenced and overexpressed in Escherichia coli BL21(DE3)
Thermus thermophilus
Inhibitors
Inhibitors
Commentary
Organism
Structure
DL-C-propagylglycine
-
Thermus thermophilus
Hydroxylamine hydrochloride
-
Thermus thermophilus
L-methionine
-
Thermus thermophilus
phenylhydrazine
-
Thermus thermophilus
Semicarbazide
-
Thermus thermophilus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3
-
H2S
-
Thermus thermophilus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
4 * 46000, calculated from OAH1 sequence
Thermus thermophilus
46059
-
4 * 46059, calculated from amino acid sequence
Thermus thermophilus
47000
-
4 * 47000, SDS-PAGE
Thermus thermophilus
163000
-
gel filtration
Thermus thermophilus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermus thermophilus
Q93I77
DDBJ: AB049221, EMBL:AB049221; HB8, HB27; nucleotide sequence accession no.
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q93I77
DDBJ: AB049221, EMBL:AB049221
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Thermus thermophilus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.06
-
cysthathione beta-lyase reaction
Thermus thermophilus
0.58
-
L-homoserine as substrate
Thermus thermophilus
0.72
-
O-succinyl-L-homoserine as substrate
Thermus thermophilus
1.33
-
L-serine as substrate
Thermus thermophilus
1.57
-
O-phospho-L-serine as substrate
Thermus thermophilus
2.12
-
O-acetyl-L-serine as substrate
Thermus thermophilus
40.7
-
recombinant enzyme
Thermus thermophilus
49.82
-
O-acetyl-L-homoserine as substrate
Thermus thermophilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-homoserine + H2S
-
637839
Thermus thermophilus
homocysteine + ?
-
-
-
?
L-homoserine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
homocysteine + ?
-
-
-
?
additional information
low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction
637839
Thermus thermophilus
?
-
-
-
-
additional information
low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
?
-
-
-
-
O-acetyl-L-serine + H2S
-
637839
Thermus thermophilus
L-cysteine + acetic acid
-
637839
Thermus thermophilus
?
O-acetyl-L-serine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
L-cysteine + acetic acid
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
?
O-phospho-L-serine + H2S
-
637839
Thermus thermophilus
?
-
-
-
?
O-phospho-L-serine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
?
-
-
-
?
O-succinyl-L-homoserine + H2S
-
637839
Thermus thermophilus
homocysteine + ?
-
-
-
?
O-succinyl-L-homoserine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
homocysteine + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 46000, calculated from OAH1 sequence; 4 * 46059, calculated from amino acid sequence; 4 * 47000, SDS-PAGE
Thermus thermophilus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Thermus thermophilus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
60
85
-
Thermus thermophilus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
90
-
very stable at high temperatures, 90% activity at 90C for 60 min at pH 7.8
Thermus thermophilus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Thermus thermophilus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
12
50% maximum activity at pH 6.3 and pH 9.3
Thermus thermophilus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Thermus thermophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
oah1 gene cloned, sequenced and overexpressed in Escherichia coli BL21(DE3)
Thermus thermophilus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Thermus thermophilus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
DL-C-propagylglycine
-
Thermus thermophilus
Hydroxylamine hydrochloride
-
Thermus thermophilus
L-methionine
-
Thermus thermophilus
phenylhydrazine
-
Thermus thermophilus
Semicarbazide
-
Thermus thermophilus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3
-
H2S
-
Thermus thermophilus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
4 * 46000, calculated from OAH1 sequence
Thermus thermophilus
46059
-
4 * 46059, calculated from amino acid sequence
Thermus thermophilus
47000
-
4 * 47000, SDS-PAGE
Thermus thermophilus
163000
-
gel filtration
Thermus thermophilus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Thermus thermophilus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.06
-
cysthathione beta-lyase reaction
Thermus thermophilus
0.58
-
L-homoserine as substrate
Thermus thermophilus
0.72
-
O-succinyl-L-homoserine as substrate
Thermus thermophilus
1.33
-
L-serine as substrate
Thermus thermophilus
1.57
-
O-phospho-L-serine as substrate
Thermus thermophilus
2.12
-
O-acetyl-L-serine as substrate
Thermus thermophilus
40.7
-
recombinant enzyme
Thermus thermophilus
49.82
-
O-acetyl-L-homoserine as substrate
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-homoserine + H2S
-
637839
Thermus thermophilus
homocysteine + ?
-
-
-
?
L-homoserine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
homocysteine + ?
-
-
-
?
additional information
low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction
637839
Thermus thermophilus
?
-
-
-
-
additional information
low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
?
-
-
-
-
O-acetyl-L-serine + H2S
-
637839
Thermus thermophilus
L-cysteine + acetic acid
-
637839
Thermus thermophilus
?
O-acetyl-L-serine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
L-cysteine + acetic acid
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
?
O-phospho-L-serine + H2S
-
637839
Thermus thermophilus
?
-
-
-
?
O-phospho-L-serine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
?
-
-
-
?
O-succinyl-L-homoserine + H2S
-
637839
Thermus thermophilus
homocysteine + ?
-
-
-
?
O-succinyl-L-homoserine + H2S
-
637839
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
homocysteine + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 46000, calculated from OAH1 sequence; 4 * 46059, calculated from amino acid sequence; 4 * 47000, SDS-PAGE
Thermus thermophilus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Thermus thermophilus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
60
85
-
Thermus thermophilus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
90
-
very stable at high temperatures, 90% activity at 90C for 60 min at pH 7.8
Thermus thermophilus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Thermus thermophilus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
12
50% maximum activity at pH 6.3 and pH 9.3
Thermus thermophilus
Other publictions for EC 2.5.1.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721161
Tran
A novel mechanism of sulfur tr ...
Wolinella succinogenes
Acta Crystallogr. Sect. D
67
831-838
2011
-
-
1
1
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4
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1
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721214
Yin
Expression, purification and p ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
67
959-963
2011
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1
1
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5
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722931
Bolten
Towards methionine overproduct ...
Corynebacterium glutamicum
J. Microbiol. Biotechnol.
20
1196-1203
2010
-
1
-
-
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-
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1
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3
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
684467
Tang
Two newly synthesized 5-methyl ...
Homo sapiens
Anticancer Drugs
19
697-704
2008
-
-
-
-
-
-
2
-
-
-
-
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-
1
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1
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2
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2
2
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687896
Ott
Polymorphisms in methionine sy ...
Homo sapiens
J. Cancer Res. Clin. Oncol.
134
405-410
2008
-
1
-
-
-
-
-
-
-
-
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-
1
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688752
Lima
Polymorphisms of methylenetetr ...
Homo sapiens
Leuk. Res.
32
401-405
2008
-
1
-
-
-
-
-
-
-
-
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1
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1
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-
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-
671277
Matityahu
Transgenic tobacco plants over ...
Saccharomyces cerevisiae
Amino Acids
30
185-194
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
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2
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1
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-
2
-
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-
-
-
-
-
-
-
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-
-
-
661358
Iwama
Comparative characterization o ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Biosci. Biotechnol. Biochem.
68
1357-1361
2004
-
-
1
-
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5
1
-
-
3
-
-
3
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1
-
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-
4
-
11
2
1
-
1
-
1
1
1
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-
1
-
-
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5
-
1
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3
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1
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4
-
11
2
1
-
1
-
1
1
1
-
-
-
-
-
-
-
661552
Qin
Cloning of the O-acetylhomoser ...
Selenomonas ruminantium, Selenomonas ruminantium HD4
CURR. MICROBIOL.
48
305-311
2004
-
-
1
-
-
-
-
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1
2
-
12
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2
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2
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1
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1
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1
2
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2
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2
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1
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662606
Yeom
-
Regulation of enzymes involved ...
Corynebacterium glutamicum
J. Microbiol. Biotechnol.
14
373-378
2004
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2
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1
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1
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637841
Hacham
In vivo analysis of various su ...
Leptospira meyeri, Saccharomyces cerevisiae
Mol. Biol. Evol.
20
1513-1520
2003
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1
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2
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7
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5
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5
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662480
Omura
Purification, characterization ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus CN3
J. BIOSCI. BIOENG.
96
53-58
2003
-
-
1
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-
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-
2
-
2
3
2
-
6
-
-
1
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-
6
-
16
2
1
-
1
-
1
-
1
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-
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-
1
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-
-
-
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2
-
2
3
2
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1
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6
-
16
2
1
-
1
-
1
-
1
-
-
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-
-
-
-
637840
Lambert
Methionine supply to growing s ...
Bos taurus
J. Nutr.
132
2004-2009
2002
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1
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11
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-
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11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637839
Shimizu
Cloning and overexpression of ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Biochim. Biophys. Acta
1549
61-72
2001
-
-
1
-
-
-
5
1
-
-
4
-
-
6
-
-
1
-
-
-
8
-
10
1
1
1
1
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
5
-
1
-
-
4
-
-
-
-
1
-
-
8
-
10
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
637838
Yamagata
Overexpression of the Saccharo ...
Saccharomyces cerevisiae
Appl. Microbiol. Biotechnol.
42
92-99
1994
-
-
1
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1
4
2
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-
1
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1
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637837
Brzywczy
Comparative studies on O-acety ...
Aspergillus nidulans, Aspergillus nidulans sG8
Acta Biochim. Pol.
40
421-427
1993
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2
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3
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5
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1
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10
1
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1
1
10
1
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637836
Yamagata
-
Purification and properties of ...
Aspergillus nidulans, Aspergillus nidulans sG8
J. Gen. Appl. Microbiol.
36
137-141
1990
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1
3
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2
2
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3
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1
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6
1
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1
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1
1
6
1
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1
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637835
Chocat
-
Synthesis of selenocystine and ...
Saccharomyces cerevisiae
Agric. Biol. Chem.
49
1143-1150
1985
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3
10
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2
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1
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1
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8
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4
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10
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8
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1
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637834
Yamagata
O-Acetylhomoserine sulfhydryla ...
Schizosaccharomyces pombe
J. Biochem.
96
1511-1523
1984
1
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1
12
4
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2
1
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2
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1
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6
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1
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1
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1
1
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1
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1
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12
1
4
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1
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1
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1
6
-
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1
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1
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1
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34702
Morinaga
-
Biosynthesis of homocysteine i ...
Pseudomonas sp., Pseudomonas sp. FM518
Agric. Biol. Chem.
47
2855-2860
1983
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2
1
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2
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2
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6
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2
1
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2
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6
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637832
Ozaki
Methionine biosynthesis in Bre ...
Brevibacterium flavum
J. Biochem.
91
1163-1171
1982
2
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4
2
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2
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1
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2
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1
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4
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1
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-
637338
Yamagata
Occurrence of low molecular we ...
Saccharomyces cerevisiae
J. Biochem.
88
1419-1423
1980
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5
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2
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637830
Murooka
O-Alkylhomoserine synthesis ca ...
Corynebacterium acetophilum, Corynebacterium acetophilum A51
J. Bacteriol.
130
62-73
1977
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1
16
4
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2
1
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4
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1
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2
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15
1
1
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1
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1
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1
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1
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16
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4
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1
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1
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2
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15
1
1
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1
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-
637331
Yamagata
O-Acetylserine and O-acetylhom ...
Saccharomyces cerevisiae
J. Biochem.
80
777-785
1976
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1
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1
2
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5
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1
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1
1
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1
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1
1
5
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637332
Yamagata
O-Acetylserine and O-acetylhom ...
Saccharomyces cerevisiae
J. Biochem.
80
787-797
1976
2
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3
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1
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637827
Yamagata
O-acetylserine and O-acetylhom ...
Saccharomyces cerevisiae
J. Biochem.
77
1029-1036
1975
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1
2
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2
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1
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637329
Yamagata
Evidence for the identity of O ...
Saccharomyces cerevisiae
J. Biochem.
75
1221-1229
1974
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2
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2
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10
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1
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10
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1
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1
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637823
Kerr
-
O-Acetylhomoserine sulfhydryla ...
Neurospora crassa
Methods Enzymol.
17
446-450
1971
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1
1
3
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1
4
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637824
Kerr
O-Acetylhomoserine sulfhydryla ...
Neurospora crassa
J. Biol. Chem.
246
95-102
1971
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1
3
3
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1
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1
6
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1
1
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1
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1
3
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1
6
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1
1
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637825
Yamagata
Homocysteine in yeast. Partial ...
Saccharomyces cerevisiae
J. Biochem.
70
1035-1045
1971
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5
1
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1
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1
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6
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1
1
1
1
4
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5
4
1
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1
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1
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6
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1
1
1
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637822
Smith
Utilization of S-methylcystein ...
Neurospora sp.
Biochim. Biophys. Acta
184
130-138
1969
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