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Information on EC 2.5.1.49 - O-acetylhomoserine aminocarboxypropyltransferase
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2.5.1.49 O-acetylhomoserine aminocarboxypropyltransferaseIUBMB Comments
Also reacts with other thiols and H2S, producing homocysteine or thioethers. The name methionine synthase is more commonly applied to EC 2.1.1.13, methionine synthase. The enzyme from baker's yeast also catalyses the reaction of EC 2.5.1.47 cysteine synthase, but more slowly.
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Word Map
- 2.5.1.49
- cystathionine
- o-acetylserine
- l-methionine
-
4.2.99.10
- o-acetyl-l-serine
-
gamma-lyase
-
gamma-synthase
- l-homocysteine
- o-succinylhomoserine
- o-acetyltransferase
- beta-synthase
-
beta-lyase
- medicine
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
o-acetylhomoserine sulfhydrylase, homocysteine synthase, o-acetyl-l-homoserine sulfhydrylase, gamma-cyano-alpha-aminobutyric acid synthase, oah sulfhydrylase, o-acetylhomoserine sulfhydrolase, o-acetylhomoserine (thiol)-lyase, oah shlase, o-acetylhomoserine aminocarboxypropyltransferase, oah-oas sulfhydrylase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AHS
EC 4.2.99.10
gamma-cyano-alpha-aminobutyric acid synthase
MetC
methionine synthase
-
-
-
-
MetY
O-acetyl-L-homoserine (thiol)-lyase
-
-
-
-
O-acetyl-L-homoserine acetate-lyase (adding methanethiol)
-
-
-
-
O-acetyl-L-homoserine sulfhydrolase
-
-
-
-
o-acetyl-L-homoserine sulfhydrylase
O-acetylhomoserine sulfhydrolase
-
-
-
-
O-acetylhomoserine sulfhydrylase
OAH
OAH SHase
OAH SHLase
oah2 gene product
OAHS
Q187D4
EC 4.2.99.10
-
-
formerly
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
O-acetyl-L-homoserine + methanethiol = L-methionine + acetate
Also reacts with other thiols and H2S, producing homocysteine or thioethers. The name methionine synthase is more commonly applied to EC 2.1.1.13 5-methyltetrahydrofolate-homocysteine S-methyltransferase. The enzyme from baker's yeast also catalyses the reaction of EC 2.5.1.47 cysteine synthase, but more slowly
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
elimination
gamma-replacement
elimination
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
O-acetyl-L-homoserine:methanethiol 3-amino-3-carboxypropyltransferase
Also reacts with other thiols and H2S, producing homocysteine or thioethers. The name methionine synthase is more commonly applied to EC 2.1.1.13, methionine synthase. The enzyme from baker's yeast also catalyses the reaction of EC 2.5.1.47 cysteine synthase, but more slowly.
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CAS REGISTRY NUMBER
COMMENTARY
37290-90-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 O-acetyl-L-homoserine + dimethyldisulfide
2 L-methionine + 2 acetate
-
dimethyldisulfide may be reductively cleaved into methanethiol prior to assimilation
-
-
?
2 O-acetyl-L-homoserine + H2S
2 L-homocysteine + 2 acetate
-
-
-
-
?
o-acetyl-L-homoserine + KCN
gamma-cyano-alpha-aminobutyric acid + acetate
-
-
-
-
?
O-acetyl-L-homoserine + methanol
O-methyl-homoserine + acetic acid
Corynebacterium acetophilum
-
-
-
?
O-acetyl-L-homoserine + n-pentanol
O-pentyl-homoserine + acetic acid
Corynebacterium acetophilum
-
-
-
?
O-acetyl-L-homoserine + S2-
L-homocysteine + acetic acid
-
physiological substrate NaHS
-
-
?
O-acetyl-L-serine + methanethiol
?
-
reaction only in phosphate buffer, relative activity compared to O-acetyl-L-homoserine 14.8%
-
-
?
O-acetyl-L-serine + methyl mercaptan
S-methylcysteine + ?
-
-
-
?
O-acetyl-L-serine + S2-
L-cysteine + acetic acid
-
physiological substrate NaHS
-
-
?
O-phosphoryl-L-serine + H2S
L-cysteine + H2O
Q76K51
lesser activity
-
-
?
L-homocysteine + KCN
gamma-cyano-alpha-aminobutyric acid + H2S
-
-
-
-
?
L-homocysteine + KCN
gamma-cyano-alpha-aminobutyric acid + H2S
-
-
-
-
?
L-homoserine + H2S
L-homocysteine + H2O
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q76K51
lesser activity
-
-
?
L-serine + H2S
L-cysteine + H2O
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q76K51
lesser activity
-
-
?
o-acetyl-L-homoserine + CN-
gamma-cyano-alpha-aminobutyric acid + acetate
-
glutamine and glutamic acid synthesis pathway
-
-
?
o-acetyl-L-homoserine + CN-
gamma-cyano-alpha-aminobutyric acid + acetate
-
glutamine and glutamic acid synthesis pathway
-
-
?
O-acetyl-L-homoserine + ethanol
O-ethyl-homoserine + acetic acid
Corynebacterium acetophilum
-
-
-
?
O-acetyl-L-homoserine + ethanol
O-ethyl-homoserine + acetic acid
Corynebacterium acetophilum A51
-
-
-
?
O-acetyl-L-homoserine + ethyl mercaptan
L-ethionine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + ethyl mercaptan
L-ethionine + acetic acid
-
-
-
?
o-acetyl-L-homoserine + H2S
L-homocysteine + acetate
AY257545
-
-
-
?
o-acetyl-L-homoserine + H2S
L-homocysteine + acetate
AY257545
-
-
-
?
o-acetyl-L-homoserine + H2S
L-homocysteine + acetate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q76K51
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
Corynebacterium acetophilum
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
direct sulfhydrylation pathway, methionine biosynthesis
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
direct sulfhydrylation pathway, biosynthesis of homocysteine
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
direct sulfhydrylation pathway, biosynthesis of homocysteine
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + hydrogen sulfide
L-homocysteine + acetate
-
-
-
?
O-acetyl-L-homoserine + hydrogen sulfide
L-homocysteine + acetate
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
methionine biosynthesis pathway
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
pathway for formation of methionine, incorporation of sulfur, possible temporary function for this enzyme may be formation of methionine from methylmercaptan at times of exhaustion of an exogenous source of sulfur
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
methionine biosynthetic pathway
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetic acid
-
relative activity 100%
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
Corynebacterium acetophilum
-
-
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
Corynebacterium acetophilum A51
-
-
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
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-
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
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-
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
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-
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + methyl mercaptan
L-methionine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + methylselenide
seleno-L-methionine + acetic acid
-
-
-
-
?
O-acetyl-L-homoserine + methylselenide
seleno-L-methionine + acetic acid
-
-
-
-
?
O-acetyl-L-homoserine + n-butanol
O-butyl-homoserine + acetic acid
Corynebacterium acetophilum
-
-
-
?
O-acetyl-L-homoserine + n-butanol
O-butyl-homoserine + acetic acid
Corynebacterium acetophilum A51
-
-
-
?
O-acetyl-L-homoserine + n-propanol
O-propyl-homoserine + acetic acid
Corynebacterium acetophilum
-
-
-
?
O-acetyl-L-homoserine + n-propanol
O-propyl-homoserine + acetic acid
Corynebacterium acetophilum A51
-
-
-
?
O-acetyl-L-serine
L-cysteine + acetate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q76K51
lesser activity
-
-
?
O-acetyl-L-serine + H2S
L-cysteine + acetic acid
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q93I77
-
-
?
O-succinyl-L-homoserine + H2S
homocysteine + ?
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q93I77
-
-
-
?
O-succinyl-L-homoserine + H2S
L-homocysteine + succinate
Q76K51
lesser activity
-
-
?
O-succinyl-L-homoserine + H2S
L-homocysteine + succinate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q76K51
lesser activity
-
-
?
additional information
?
-
Corynebacterium acetophilum
-
O-acetyl-L-serine, L-homoserine, O-succinyl-L-homoserine and O-acetyl-L-threonine are no substrates
-
-
?
additional information
?
-
Corynebacterium acetophilum A51
-
O-acetyl-L-serine, L-homoserine, O-succinyl-L-homoserine and O-acetyl-L-threonine are no substrates
-
-
?
additional information
?
-
-
no cystathione formed from cysteine, no alpha-ketobutyrate formed from O-acetylhomoserine, negligible activity with homoserine and serine, O-succinylhomoserine, O-phosphorylhomoserine, O-acetylserine and O-phosphorylserine are no substrates
-
-
?
additional information
?
-
-
L-homoserine, O-succinyl-DL-homoserine, O-phosphoryl-L-homoserine, L-serine, O-acetyl-L-serine, O-phosphoryl-L-serine react at less than 1% the rate with O-acetyl-L-homoserine
-
-
?
additional information
?
-
-
L-homoserine and O-succinyl-L-homoserine are no substrates
-
-
?
additional information
?
-
-
L-homoserine and O-succinyl-L-homoserine are no substrates
-
-
?
additional information
?
-
-
O-acetyl-L-threonine, O-acetyl-L-tyrosine, O-acetyl-L-hydroxyproline and O-succinyl-DL-homoserine are no substrates
-
-
?
additional information
?
-
-
O-acetyl-L-threonine, O-acetyl-L-tyrosine, O-acetyl-L-hydroxyproline and O-succinyl-DL-homoserine are no substrates
-
-
?
additional information
?
-
-
cystathionine is not synthesized from O-acetylhomoserine and cysteine, O-acetyl-L-serine is no substrate
-
-
?
additional information
?
-
-
the enzyme sulfhydrylates both O-acetyl-L-homoserine and O-acetylserine using sulfide to yield homocysteine and cysteine, respectively
-
-
?
additional information
?
-
-
does not synthesize cystathionine from O-acetyl-L-homoserine and L-cysteine, cannot utilize cysteine as co-substrate in place of H2S, O-acetyl-L-serine, O-acetyl-L-tyrosine, O-acetyl-L-threonine, O-phospho-DL-threonine, O-phospho-L-serine, L-serine-O-sulfate and O-acetyl-L-hydroxyproline are no substrates
-
-
?
additional information
?
-
Q93I77
low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction
-
-
?
additional information
?
-
-
low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction
-
-
?
additional information
?
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Q93I77
low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction
-
-
?
additional information
?
-
-
shows with O-acetylserine 1% of the activity with O-acetylhomoserine, no activity with O-succinyl-L-homoserine, homoserine or serine
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
O-acetyl-L-homoserine + S2-
L-homocysteine + acetic acid
-
physiological substrate NaHS
-
-
?
O-acetyl-L-serine + S2-
L-cysteine + acetic acid
-
physiological substrate NaHS
-
-
?
o-acetyl-L-homoserine + CN-
gamma-cyano-alpha-aminobutyric acid + acetate
-
glutamine and glutamic acid synthesis pathway
-
-
?
o-acetyl-L-homoserine + CN-
gamma-cyano-alpha-aminobutyric acid + acetate
-
glutamine and glutamic acid synthesis pathway
-
-
?
o-acetyl-L-homoserine + H2S
L-homocysteine + acetate
AY257545
-
-
-
?
o-acetyl-L-homoserine + H2S
L-homocysteine + acetate
AY257545
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
Corynebacterium acetophilum
-
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
direct sulfhydrylation pathway, methionine biosynthesis
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
direct sulfhydrylation pathway, biosynthesis of homocysteine
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
direct sulfhydrylation pathway, biosynthesis of homocysteine
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
-
?
O-acetyl-L-homoserine + H2S
L-homocysteine + acetic acid
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
methionine biosynthesis pathway
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
pathway for formation of methionine, incorporation of sulfur, possible temporary function for this enzyme may be formation of methionine from methylmercaptan at times of exhaustion of an exogenous source of sulfur
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
-
-
-
?
O-acetyl-L-homoserine + methanethiol
L-methionine + acetate
-
methionine biosynthetic pathway
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate