Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1S,3aS,4R,6aR)-1,4-bis(1,3-benzodioxol-5-yl)hexahydropentalen-3a(1H)-ol | i.e. paulownin, NPLC604, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 15 mg/ml | Helicobacter pylori | |
(1S,3aS,4S,6aS)-1,4-di(3,4,5-trimethoxy)-phenyloctahydropentalene | i.e. yangambin, NPLC605, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 10 mg/ml | Helicobacter pylori | |
2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione | i.e. alpha-lapachone, NPLC518, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 2.6 mg/ml | Helicobacter pylori | |
6-hydroxy-2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione | i.e. 9-hydroxy-alpha-lapachone, NPLC519, noncompetitive, significant activity in the inhibition of Helicobacter pylori strain SS1 growth with MIC of 2.5 mg/ml | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.02 | - |
O-succinyl-L-homoserine | 25°C, pH 9.0 | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | Q1M0P5 | - |
- |
Helicobacter pylori SS | Q1M0P5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Helicobacter pylori |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.403 | - |
25°C, pH 9.0 | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-succinyl-L-homoserine + H2O | - |
Helicobacter pylori | succinate + 2-oxobutyrate + NH3 | - |
? | |
O-succinyl-L-homoserine + H2O | - |
Helicobacter pylori SS | succinate + 2-oxobutyrate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.297 | - |
O-succinyl-L-homoserine | 25°C, pH 9.0 | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Helicobacter pylori |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
6-hydroxy-2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione | 25°C, pH 9.0 | Helicobacter pylori | |
0.019 | - |
2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione | 25°C, pH 9.0 | Helicobacter pylori | |
0.031 | - |
(1S,3aS,4S,6aS)-1,4-di(3,4,5-trimethoxy)-phenyloctahydropentalene | 25°C, pH 9.0 | Helicobacter pylori | |
0.06 | - |
(1S,3aS,4R,6aR)-1,4-bis(1,3-benzodioxol-5-yl)hexahydropentalen-3a(1H)-ol | 25°C, pH 9.0 | Helicobacter pylori |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.009 | - |
25°C, pH 9.0 | Helicobacter pylori | 6-hydroxy-2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione | |
0.011 | - |
25°C, pH 9.0 | Helicobacter pylori | 2,2-dimethyl-3,4-dihydro-2H-benzo[g]chromene-5,10-dione | |
0.019 | - |
25°C, pH 9.0 | Helicobacter pylori | (1S,3aS,4R,6aR)-1,4-bis(1,3-benzodioxol-5-yl)hexahydropentalen-3a(1H)-ol | |
0.027 | - |
25°C, pH 9.0 | Helicobacter pylori | (1S,3aS,4S,6aS)-1,4-di(3,4,5-trimethoxy)-phenyloctahydropentalene |